The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis

BACKGROUND: As only a minor portion of the information present in histological sections is accessible by eye, recognition and quantification of complex patterns and relationships among constituents relies on digital image analysis. In this study, our working hypothesis was that, with the application of digital image analysis technology, visually unquantifiable breast cancer microarchitectural features can be rigorously assessed and tested as prognostic parameters for invasive breast carcinoma of no special type. METHODS: Digital image analysis was performed using public domain software (ImageJ) on tissue microarrays from a cohort of 696 patients, and validated with a commercial platform (Visiopharm). Quantified features included elements defining tumour microarchitecture, with emphasis on the extent of tumour-stroma interface. The differential prognostic impact of tumour nest microarchitecture in the four immunohistochemical surrogates for molecular classification was analysed. Prognostic parameters included axillary lymph node status, breast cancer-specific survival, and time to distant metastasis. Associations of each feature with prognostic parameters were assessed using logistic regression and Cox proportional models adjusting for age at diagnosis, grade, and tumour size. RESULTS: An arrangement in numerous small nests was associated with axillary lymph node involvement. The association was stronger in luminal tumours (odds ratio (OR) = 1.39, p = 0.003 for a 1-SD increase in nest number, OR = 0.75, p = 0.006 for mean nest area). Nest number was also associated with survival (hazard ratio (HR) = 1.15, p = 0.027), but total nest perimeter was the parameter most significantly associated with survival in luminal tumours (HR = 1.26, p = 0.005). In the relatively small cohort of triple-negative tumours, mean circularity showed association with time to distant metastasis (HR = 1.71, p = 0.027) and survival (HR = 1.8, p = 0.02). CONCLUSIONS: We propose that tumour arrangement in few large nests indicates a decreased metastatic potential. By contrast, organisation in numerous small nests provides the tumour with increased metastatic potential to regional lymph nodes. An outstretched pattern in small nests bestows tumours with a tendency for decreased breast cancer-specific survival. Although further validation studies are required before the argument for routine quantification of microarchitectural features is established, our approach is consistent with the demand for cost-effective methods for triaging breast cancer patients that are more likely to benefit from chemotherapy

Diagnostic yield of next-generation sequencing in very early-onset inflammatory bowel diseases: a multicenter study (vol 12, pg 1104, 2021)

Transplantation and immunomodulatio

Prevalence and Intensity of Sarcocystis spp. Microscopic Cysts in Sheep Muscles...In: Israel Journal of Veterinary Medicine - Vol. 69 - N°1 - Mar. 2014

Percoll gradient centrifugation can purify different parasitic stages from tissues or faeces of hosts. Sarcocystis tenella and Sarcocystis arieticanis develop microscopically visible cysts in sheep muscles. In this study, the distribution of microscopic cysts were determined in different muscles groups of sheep naturally infected with Sarcocystis spp. by using percoll gradient centrifugation. Sarcocystis spp. microscopic cysts were detected as 91% of sheep. S.tenella and mixed infections with S.tenella and S.arieticanis were observed in the tissue samples at a prevalence of 91% and 18.7%, respectively. Sarcocystis species cysts were most prevalent in the tongue muscle tissue at a rate of 80%. The cysts were observed a rate of 73%, 69% and 61% in the masseter, intercostal muscles and diaphragm, respectively. The relationship between the cysts present and the different muscles groups was significantly different (p<0.001). The number of microscopic cysts ranged from 4-476 (mean 235) per 5 grams muscle samples. Percoll gradient centrifugation should be considered as an alternative method for detection of Sarcocystis spp. microscopic cysts in host muscles

Prevalence and Concentration of Sarcocystis spp. Microscopic Cysts in Sheep Muscles Using Percoll Gradient Centrifugation

YILDIZ, Kader/0000-0001-5802-6156WOS: 000332720200003Percoll gradient centrifugation can purify different parasitic stages from tissues or faeces of hosts. Sarcocystis tenella and Sarcocystis arieticanis develop microscopically visible cysts in sheep muscles. In this study, the distribution of microscopic cysts were determined in different muscles groups of sheep naturally infected with Sarcocystis spp. by using percoll gradient centrifugation. Sarcocystis spp. microscopic cysts were detected as 91% of sheep. S. tenella and mixed infections with S. tenella and S. arieticanis were observed in the tissue samples at a prevalence of 91% and 18.7%, respectively. Sarcocystis species cysts were most prevalent in the tongue muscle tissue at a rate of 80%. The cysts were observed a rate of 73%, 69% and 61% in the masseter, intercostal muscles and diaphragm, respectively. The relationship between the cysts present and the different muscles groups was significantly different (p<0.001). The number of microscopic cysts ranged from 4-476 (mean 235) per 5 grams muscle samples. Percoll gradient centrifugation should be considered as an alternative method for detection of Sarcocystis spp. microscopic cysts in host muscles.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 110O497]This work was supported by a grant from TUBITAK (project no: TOVAG 110O497)

Body weight and body measurements of male and female Pekin ducks obtained from breeder flocks of different age

Onbasilar, Ebru/0000-0002-1321-0280;WOS: 000298510800009In total, 144 Pekin ducklings (72 male and 72 female) obtained from breeder flocks of different ages (37 and 135 wks) were used to evaluate body weight and body measurements until 42 days of life. Ducklings were leg-banded for individual identification after hatching and sexing. Body weight and body measurements were taken at hatch and during the 6 weeks experimental period. Time - breeder age and. time - sex interactions were found to be significant (p < 0.001). Growth model of female ducks was modeled with a three parameters logistic regression, whereas, male ducks were modeled with Gompertz model. At hatch, age of the breeder flock and sex affected all of the examined parameters however at slaughter age only body weight was affected. Male ducks were significantly heavier than female ducks at slaughter age

The Comparison of Growth Curve with Different Models in Anatolian Buffalo

WOS: 000453168100007The aim of this study is determining the relationship between body weight and age on growth curve models and selecting the best fitted model. The study was conducted in live weight records maintained a total of 54 male and female buffalo heads for a year. Richards, Gompertz, Logistic and Von Bertalanffy models are used as growth model in recent study. The model parameters were calculated and the comparisons among the models are materialized according to goodness of fit criteria (R-2, R-adj(2), MSE, AIC and BIC). As a result, all models were indicated that high and similar goodness of fit criteria. Richards and Von Bertalanffy models are the most appropriate (R-2=0,996; R-adj(2)=0,993; MSE=62,71; AIC=42,47; BIC=27,29) for female animal and (R-2=0,998; R-d(2)=0,998; HKO=18,51; AIC=30,51; BIC=12,65) male animal, respectively