Fungicide-resistant os mutants of Neurospora crassa

Fungicide-resistant os mutants of Neurospora crass

Fungicide resistance of smco mutants of Neurospora crassa.

Fungicide resistance of smco mutants of Neurospora crassa

Variations among extrachromosomal mutants of N. crassa

Variations among extrachromosomal mutant

The Politics of Service Delivery Reform

This article identifies the leaders, the supporters and the resisters of public service reform. It adopts a principal–agent framework, comparing reality with an ‘ideal’ situation in which citizens are the principals over political policy-makers as their agents, and policy-makers are the principals over public service officials as their agents. Reform in most developing countries is complicated by an additional set of external actors — international financial institutions and donors. In practice, international agencies and core government officials usually act as the ‘principals’ in the determination of reforms. The analysis identifies the interests involved in reform, indicating how the balance between them is affected by institutional and sectoral factors. Organizational reforms, particularly in the social sectors, present greater difficulties than first generation economic policy reforms

Game of Stones:feasibility randomised controlled trial of how to engage men with obesity in text message and incentive interventions for weight loss

Objectives To examine the acceptability and feasibility of narrative text messages with or without financial incentives to support weight loss for men. Design Individually randomised three-arm feasibility trial with 12 months’ follow-up. Setting Two sites in Scotland with high levels of disadvantage according to Scottish Index for Multiple Deprivation (SIMD). Participants Men with obesity (n=105) recruited through community outreach and general practitioner registers. Interventions Participants randomised to: (A) narrative text messages plus financial incentive for 12 months (short message service (SMS)+I), (B) narrative text messages for 12 months (SMS only), or (C) waiting list control. Outcomes Acceptability and feasibility of recruitment, retention, intervention components and trial procedures assessed by analysing quantitative and qualitative data at 3, 6 and 12 months. Results 105 men were recruited, 60% from more disadvantaged areas (SIMD quintiles 1 or 2). Retention at 12 months was 74%. Fewer SMS+I participants (64%) completed 12-month assessments compared with SMS only (79%) and control (83%). Narrative texts were acceptable to many men, but some reported negative reactions. No evidence emerged that level of disadvantage was related to acceptability of narrative texts. Eleven SMS+I participants (31%) successfully met or partially met weight loss targets. The cost of the incentive per participant was £81.94 (95% CI £34.59 to £129.30). Incentives were acceptable, but improving health was reported as the key motivator for weight loss. All groups lost weight (SMS+I: −2.51 kg (SD=4.94); SMS only: −1.29 kg (SD=5.03); control: −0.86 kg (SD=5.64) at 12 months). Conclusions This three-arm weight management feasibility trial recruited and retained men from across the socioeconomic spectrum, with the majority from areas of disadvantage, was broadly acceptable to most participants and feasible to deliver

Teaching early numeracy to students with autism using a school staff delivery model

Mathematics is one of the core school subjects in the UK and an emphasis is placed on developing pupils’ mathematical competencies throughout all key stages. Despite that, the attainment of students with disabilities in mathematics remains low. The current study explored ways in which the Teaching Early Numeracy to children with Developmental Disabilities (TEN-DD) programme could be implemented by teaching staff in a special school in the UK to improve the numeracy skills of students with autism. Adaptations to the delivery of the programme were made during the study as a result of continued collaboration with the participating school. The findings suggest that it may be feasible to implement the TEN-DD programme using a school staff delivery model and it may help learners improve their early numeracy skills. Practical aspects of TEN-DD’s implementation highlighted the need to incorporate more systematic adaptations for minimally verbal students, as well as for learners who might need additional training with prerequisite skills

Assessment of Muscle Activation of Caregivers Performing Dependent Transfers With a Novel Robotic-Assisted Transfer Device Compared With the Hoyer Advance

OBJECTIVE: The purpose of this study was to compare muscle activity in caregivers while using a novel robotic assistive transfer device (Strong Arm) to a clinical standard of care (Hoyer Advance). DESIGN: A Quasi-Experimental design was used in which twenty caregivers (33±15 years old) performed transfers with three surfaces (toilet, bench and shower chair) with the Strong Arm and Hoyer Advance. Transfer completion time (sec), peak percentage surface electromyography (EMG) and integrated EMG of the bilateral erector spinae, latissimus dorsi, pectoralis major and anterior deltoid were measured. RESULTS: Caregivers required less transfer time when transferring from wheelchair to surface using the Hoyer Advance (p=.011, f=.39). Lower back: significantly lower pEMG were found using Strong Arm in 50% and for the iEMG in 25% of the cases, with the remaining cases showing no significant differences. Shoulder: significantly lower pEMG were found using Strong Arm in 19% of transfers and lower iEMG was found in 25% of transfers when using the Hoyer Advance, with the remaining cases showing no significant differences. CONCLUSION: While back muscle activation during Strong Arm transfers is statistically, but not clinically, lower, additional features that couple with significantly lower muscle activation make it an alternative to the clinical standard for further research and possible clinical applicability

Student research

CLOSTRIDIUM OEDEMATIENSN.H.S. WIGSVASCULAR DISEASESGOT LEVELS IN TUBERCULOUS PATIENTS TREATED WITH PAS AND ISONIAZI

Do Development Economists Matter?

SUMMARY If appropriate policies, rather than initial economic conditions, have produced successful development, why are appropriate policies not more widely adopted by developing country governments — or, why has the advice of most development economists not been heeded? The ‘new (neoclassical) political economy’ offers a systematic explanation of why policy?makers behave as they do. Instead of assuming that governments are agencies for promoting the public interest, the new political economy's models endogenise the policy?maker in states characterised variously as predatory (‘the Leviathan state’), as factional, or as bureaucratic. The limitations of these models are addressed, and their relevance to developing countries is questioned. A more eclectic approach to political economy is, therefore, suggested, in which older elements are combined with the new. Such an approach is then applied to the political economy of trade policy. RESUME Les économistes spécialisés dans le domaine du développement sont?ils importants? Si des mesures appropriées, plutôt que des conditions économiques initiales ont produit un développement qui a eu du succès, pourquoi des mesures adéquates ne sont?elles pas adoptées plus largement par les gouvernements des pays en voie de développement. — ou pourquoi le besoin de l'assistance de la plupart des economistes spécialisés dans les problèmes de développement ne s'est?elle pas fait sentir? La nouvelle politique économique (néo?classique) offre une explication systématique expliquant pourquoi ceux qui prennent les décisions se comportent comme ils le font. Au lieu d'assumer que les gouvernements sont des agences pour la promotion de l'intérêt publique, les nouveaux modèles d'économie politique endogénisent ceux qui prennent les décisions, les charactêrisant de prédateurs (‘l’état du Leviathan'), de fonctionel, ou de bureaucratique. Les limitations de ces modèles sont adressés, et leur pertinence concernant les pays en voie de développement sont mis en question. Une approche plus ecclectique à l'economie politique est donc, suggérée, dans laquelle des éléments plus anciens sont associés avec les nouveaux. Une telle approche est ensuite appliquée à l'économie politique du commerce. RESUMEN ¿Importan los economistas del desarrollo? Si el desarrollo exitoso ha sido producido más bien por políticas adecuadas que por condiciones económicas iniciales ¿por qué aquellas no son más ampliamente adoptadas por los gobiernos de los países en desarrollo o por qué no ha sido escuchado el consejo de muchos economistas del desarollo? La “nueva política económica (neoclásica)” ofrece una explicación sistemática del comportamiento de los diseñadores de política. En lugar de suponer que los gobiernos son agencias para promover el interés público, los nuevos modelos de economía política endogenizan el diseño de política en estados caracterizados como predatorios (“el estado Leviathan”), faccionales o burocráticos. Se consideran las limitaciones de estos modelos y se cuestiona su relevancia para los países en desarrollo. En consecuencia, se sugiere un enfoque más ecléctico que combina elementos antiguos con los nuevos aplicándose luego tal enfoque a la política comercial

Standardization and performance evaluation of mononuclear cell cytokine secretion assays in a multicenter study

BACKGROUND: Cryopreservation of peripheral blood mononuclear cells has been used to preserve and standardize immunologic measurements for multicenter studies, however, effects of cryopreservation on cytokine responses are incompletely understood. In designing immunologic studies for a new multicenter birth cohort study of childhood asthma, we performed a series of experiments to determine the effects of two different methods of cryopreservation on the cytokine responses of cord and peripheral blood mononuclear cells. RESULTS: Paired samples of PBMC were processed freshly, or after cryopreservation in a Nalgene container (NC) or a controlled-rate freezer (CRF). Although there were some differences between the methods, cryopreservation inhibited PHA-induced IL-10 secretion and Der f 1-induced IL-2 secretion, and augmented PHA-induced IL-2 secretion and spontaneous secretion of TNF-α. In separate experiments, NC cryopreservation inhibited secretion of several cytokines (IL-13, IL-10, IFN-γ, TNF-α) by PHA-stimulated cord blood mononuclear cells. With the exception of PHA-induced IL-13, results from fresh and cryopreserved cord blood samples were not significantly correlated. Finally, in reproducibility studies involving processing of identical cell samples in up to 4 separate laboratories, variances in cytokine responses of fresh cells stimulated at separate sites did not exceed those in cryopreserved cells stimulated at a central site. CONCLUSION: Collectively, these studies indicate that cryopreservation can affect mononuclear cell cytokine response profiles, and that IL-10 secretion and antigen-induced responses may be especially vulnerable. These studies also demonstrate that mononuclear cell responses can be standardized for performance in a small number of laboratories for multicenter studies, and underscore the importance of measuring reproducibility and of testing whether cryopreservation techniques alter specific immunologic outcomes