Features of the immune profile and microbiota in persons whose immune status is susceptible or resistant to chronic stress

Background. The presence of influence both sympathetic and vagus links of the autonomic nervous system to the immune system is generally recognized, but the data on their specific immunotropic effects are ambiguous. This includes, in particular, immune responses to the stress-induced shift of sympatho-vagal balance. The purpose of this study is to identify the characteristics of the immune profile, as well as the microbiota associated with it, in persons whose immune status is susceptible or resistant to chronic stress. Materials and Methods. The object of observation were 32 men and 8 women with chronic pyelonephritis in remission. The criterion for inclusion was the magnitude of the sympatho-vagal balance index LF/HF (recorded by "CardioLab+HRV"), which exceeded the age norm by 0,5 σ. Immune status evaluated on a set of I and II levels recommended by the WHO. The observed contingent by pair matching of persons with the same values of LF/HF ratio was retrospectively divided into two groups, almost identical to the average value of LF/HF and its dispersion, but with opposite deviations from the norm of the immune status index. Results. Immune profiles constructed on Z-scores can be divided into three networks. The first set contains 8 parameters (Killing Index vs E. coli and Staph. aur., IL-6, Entropy of LCG, Popovych’s Strain Index of LCG, Igg G and M as well as Microbial Count E. coli) that are not significantly different from persons who are stress-sensitive and stress-resistant. 18 parameters of the second set (Leukocytes, Phagocytose Index vs E. coli and Staph. aur.(PhIA), B-Lymphocytes, Segmented Neutrophils, Microbial Count Staph. aur., T-active (Ta), T-cytolytic and Natural Killers Lymphocytes (NK), IgA, Bactericidity vs E. coli and Staph. aur. (BCA), Bifidobacterium and Lactobacillus feces, Stub Neutrophils, T-helpers, Popovych’s Adaptation Index of LCG (PAI) as well as CIC) to a greater or lesser degree higher in stress-resistant persons. Instead, the 8 parameters of the third set (Lymphocytes, Monocytes and Eosinophils (E), TNF-α, IL-1 as well as Popovych’s Strain Index of LCG, 0-Lymphocytes and Hemolytica E. coli HEC) are higher in stress-sensitive persons. The method of discriminant analysis revealed 9 parameters (ranked by criterion Λ: 0-Lym, BCA, HEC, Ta, CIC, E, PhIA, PAI, NK) that characterize the features of immune profile of stress-susceptible and stress-resistant persons

Features of neuro-endocrine and immune reactions to various water-salt loads in female rats

Background. Previously, we have shown that the weekly load of rats with water-salt solutions of different chemical compositions causes both general and specific reactions of the parameters of metabolism. The purpose of this study is to identify under these conditions specific neuroendocrine and immune responses. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats some neuroendocrine and immune parameters were registered. Results. The method of discriminant analysis revealed 29 parameters of the neuroendocrine-immune complex (10 of them reflect the neuroendocrine regulation, 4 thymus mass and thymocytogram elements, 5 elements of splenocytogram, 10 elements of immunocytogram and leukocytogram of blood and parameters of phagocytosis), according to which the reaction on various water-salt loads are identified with an accuracy of 98.3%. Conclusion. The peculiarities of the reactions of the parameters of the neuroendocrine-immune complex are due to the content of water in sulfate, bicarbonate and magnesium, as well as organic carbon and nitrogen

Mineral waters, metabolism, neuro-endocrine-immune complex, s. 252.

Dedicated to the 75th anniversary of Volodymyr Illich Kozyavkin with gratitude for the support of the Truskavetsian Scientific School of BalneologyThe monograph systematizes these writers and highlights the results of their own priority experimental and clinical-physiological studies of the impact of drinking mineral waters of Ukraine on neuroendocrine regulation, metabolism and immunity of healthy rats and patients in the process of rehabilitation of chronic pyelonephritis and cholecystitis in remission. In line with the concepts of functional-metabolic continuum and neuroendocrine-immune complex using the methods of factor, discriminant and canonical correlation analysis, it is demonstrated that mineral waters have both similar and specific physiologically favorable modulating effects on the parameters of the studied body systems. For specialists in medical rehabilitation, endocrinologists, immunologists, biochemists, pathophysiologists