Effects of zoledronic acid and dexamethasone on early phases of socket healing after tooth extraction in rats : a preliminary macroscopic and microscopic quantitative study

The exact pathogenesis of medication-related osteonecrosis of the jaw (MRONJ) is still unknown. The aim of this paper was to investigate the effects of zoledronic acid and dexamethasone on the early phases of socket healing in rats subjected to tooth extractions. Thirty male Sprague-Dawley rats were divided into 2 groups: pharmacologically treated group (T, n=20) and non-pharmacologically treated group (C, n=10). T group rats received 0.1 mg/Kg of zoledronic acid (ZOL) and 1 mg/Kg of dexamethasone (DEX) three times a week for 10 consecutive weeks. C group rats were infused with vehicle. After 9 weeks from the first infusion, first maxillary molars were extracted in each of the rats. Quantitative macroscopic and microscopic analysis was performed to evaluate socket healing 8 days after extraction. Pharmacologically treated rats showed significant inhibition of bone remodeling. Connective tissue/alveolar bone ratio, osteoclast number and woven bone deposition were significantly reduced in group T compared to group C. Conversely, the proportion of necrotic bone was higher in group T compared to group C (0.8% and 0.3%, respectively. P=0.031). ZOL plus DEX do not cause gross effects on socket healing at a macroscopic level. Our findings confirmed that exposure to ZOL plus DEX impairs alveolar wound repair. Inhibition of osteoclastic resorption of socket walls after tooth extraction and the inability to dispose of the necrotic bone may be considered the initial steps of MRONJ onset

Hyaluronic acid enhance polynucleotides effect on cultured dermal fibroblasts

Polynucleotides (PNs) and Hyaluronic Acid (HA) are compounds widely used to promote tissue regeneration, mainly in cutis and cartilage. PNs efficacy has been demonstrated in vitro on cultured dermal fibroblasts and osteoblasts, and in animal models where it has been demonstrated to increase dermal regeneration. PNs effects are mediated mainly through the activation of P2P purinergic receptors, which are expressed on fibroblasts and on mesenchymal-derived cells. HA, the most abundant and non solphorate glycosaminoglycan (GAG) produced by fibroblasts, is envolved in several biological effects which are different in response of the sizes of HA molecules. When used as non fragmented, (about 2 Kd) HA has both an anti-inflammatory and antiapoptotic effects and it stimulates cell migration. In this study we have analyzed the effect of a mixture of PNs and HA, in order to verify a possible synergic effect, on human dermal fibroblasts. Effects on cell proliferation were evaluated with MTT assay and cell culture protein content. Dose-response curves showed higher effects on cell proliferation when PNs were used in the presence of HA. In particular we observed that, the addiction of HA determined a peak of activity with a reduction of of about a third of PNs dose. These preliminary data are suggesting for a joint use of HA and PN in tissue regeneration, mainly in clinical situation, like for example cutaneous burn in with the presence of PN induce a more rapid regeneration by means of more rapid cell ingrowth, collagen and VEGF production. At the same time HA support cell migration and contribute to reduce inflammatory processes

A New Technique for Deep in situ Measurements of the Soil Water Retention Behaviour

In situ measurements of soil suction and water content in deep soil layers still represent an experimental challenge. Mostly developed within agriculture-related disciplines, field techniques for the identification of soil retention behaviour have been so far employed in the geotechnical context to monitor shallow landslides and seasonal volume changes beneath shallow foundations, within the most superficial ground strata. In this paper, a novel installation technique is presented, discussed and assessed, which allows extension of the use of commercially available low-cost and low-maintenance instruments to characterise deep soil layers. Multi-depth installations have been successfully carried out using two different sensor types to measure the soil suction and water content up to 7\u2009m from the soil surface. Preliminary laboratory investigations were also shown to provide a reasonable benchmark to the field data. The results of this study offer a convenient starting point to accommodate important geotechnical works such as river and road embankments in the traditional monitoring of unsaturated soil variables

Eosinophil as a cellular target of the ocular anti-allergic action of mapracorat, a novel selective glucocorticoid receptor agonist

Abstract: Purpose: Glucocorticoids can either suppress gene transcription (transrepression) or activate it (transactivation). This latter process may contribute to certain side effects caused by these agents. Mapracorat (also known as BOL-303242-X or ZK 245186) is a novel selective glucocorticoid receptor agonist that maintains a beneficial anti-inflammatory activity but seems to be less effective in transactivation, resulting in a lower potential for side effects; it has been proposed for the topical treatment of inflammatory skin disorders. This study assessed the anti-allergic activity of mapracorat at the ocular level and whether eosinophils and mast cells are targets of its action

Patient specific ankle-foot orthoses using rapid prototyping

Background Prefabricated orthotic devices are currently designed to fit a range of patients and therefore they do not provide individualized comfort and function. Custom-fit orthoses are superior to prefabricated orthotic devices from both of the above-mentioned standpoints. However, creating a custom-fit orthosis is a laborious and time-intensive manual process performed by skilled orthotists. Besides, adjustments made to both prefabricated and custom-fit orthoses are carried out in a qualitative manner. So both comfort and function can potentially suffer considerably. A computerized technique for fabricating patient-specific orthotic devices has the potential to provide excellent comfort and allow for changes in the standard design to meet the specific needs of each patient. Methods In this paper, 3D laser scanning is combined with rapid prototyping to create patient-specific orthoses. A novel process was engineered to utilize patient-specific surface data of the patient anatomy as a digital input, manipulate the surface data to an optimal form using Computer Aided Design (CAD) software, and then download the digital output from the CAD software to a rapid prototyping machine for fabrication. Results Two AFOs were rapidly prototyped to demonstrate the proposed process. Gait analysis data of a subject wearing the AFOs indicated that the rapid prototyped AFOs performed comparably to the prefabricated polypropylene design. Conclusions The rapidly prototyped orthoses fabricated in this study provided good fit of the subject's anatomy compared to a prefabricated AFO while delivering comparable function (i.e. mechanical effect on the biomechanics of gait). The rapid fabrication capability is of interest because it has potential for decreasing fabrication time and cost especially when a replacement of the orthosis is required

Puzzling large-scale polarization in the galaxy cluster Abell 523

Large-scale magnetic fields reveal themselves through diffuse synchrotron sources observed in galaxy clusters such as radio halos. Total intensity filaments of these sources have been observed in polarization as well, but only in three radio halos out of about one hundred currently known. In this paper we analyze new polarimetric Very Large Array data of the diffuse emission in the galaxy cluster Abell 523 in the frequency range 1-2 GHz. We find for the first time evidence of polarized emission on scales of ~ 2.5 Mpc. Total intensity emission is observed only in the central part of the source, likely due to observational limitations. To look for total intensity emission beyond the central region, we combine these data with single-dish observations from the Sardinia Radio Telescope and we compare them with multi-frequency total intensity observations obtained with different instruments, including the LOw Frequency ARray and the Murchison Widefield Array. By analysing the rotation measure properties of the system and utilizing numerical simulations, we infer that this polarized emission is associated with filaments of the radio halo located in the outskirts of the system, in the peripheral region closest to the observer.Comment: 14 pages, 12 figures, accepted for publication on MNRA