7 research outputs found
Motivating knowledge sharing
Research Paper Series (National University of Singapore. Faculty of Business Administration); 2005-0161-1
Something in it for everyone : the neglected role of self-interest in organizational citizenship behavior
Research Paper Series (National University of Singapore. Faculty of Business Administration); 2005-0141-3
Relinquishing the 'secret of fire' : the impact of incentives and self-monitoring on knowledge sharing in organizations
Research Paper Series (National University of Singapore. Faculty of Business Administration); 2005-01
The impact of performance appraisal on knowledge sharing among employees
Research Paper Series (National University of Singapore. Faculty of Business Administration); 2005-01
When the going gets tough : goals and goal orientation on returns, risk-taking and persistence
Research Paper Series (National University of Singapore. Faculty of Business Administration); 2005-0181-4
Mapping the phosphoinositide-binding site on chick cofilin explains how PIP2 regulates the cofilin-actin interaction
Cofilin plays a key role in the choreography of actin dynamics via its ability to sever actin filaments and increase the rate of monomer dissociation from pointed ends. The exact manner by which phosphoinositides bind to cofilin and inhibit its interaction with actin has proven difficult to ascertain. We determined the structure of chick cofilin and used NMR chemical shift mapping and structure-directed mutagenesis to unambiguously locate its recognition site for phosphoinositides (Pis). This structurally unique recognition site requires both the acyl chain and head group of the PI for a productive interaction, and it is not inhibited by phosphorylation of cofilin. We propose that the interaction of cofilin with membrane-bound Pis abrogates its binding to both actin and actin-interacting protein 1, and facilitates spatiotemporal regulation of cofilin activity