RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses

HIV-1 broadly neutralizing antibodies (bnAbs) are difficult to induce with vaccines but are generated in ∼50% of HIV-1-infected individuals. Understanding the molecular mechanisms of host control of bnAb induction is critical to vaccine design. Here, we performed a transcriptome analysis of blood mononuclear cells from 47 HIV-1-infected individuals who made bnAbs and 46 HIV-1-infected individuals who did not and identified in bnAb individuals upregulation of RAB11FIP5, encoding a Rab effector protein associated with recycling endosomes. Natural killer (NK) cells had the highest differential expression of RAB11FIP5, which was associated with greater dysregulation of NK cell subsets in bnAb subjects. NK cells from bnAb individuals had a more adaptive/dysfunctional phenotype and exhibited impaired degranulation and cytokine production that correlated with RAB11FIP5 transcript levels. Moreover, RAB11FIP5 overexpression modulated the function of NK cells. These data suggest that NK cells and Rab11 recycling endosomal transport are involved in regulation of HIV-1 bnAb development. Generation of broadly neutralizing antibodies against HIV-1 in humans is linked to the expression of a specific recycling endosome-associated effector in natural killer cells

The Physics of Star Cluster Formation and Evolution

© 2020 Springer-Verlag. The final publication is available at Springer via https://doi.org/10.1007/s11214-020-00689-4.Star clusters form in dense, hierarchically collapsing gas clouds. Bulk kinetic energy is transformed to turbulence with stars forming from cores fed by filaments. In the most compact regions, stellar feedback is least effective in removing the gas and stars may form very efficiently. These are also the regions where, in high-mass clusters, ejecta from some kind of high-mass stars are effectively captured during the formation phase of some of the low mass stars and effectively channeled into the latter to form multiple populations. Star formation epochs in star clusters are generally set by gas flows that determine the abundance of gas in the cluster. We argue that there is likely only one star formation epoch after which clusters remain essentially clear of gas by cluster winds. Collisional dynamics is important in this phase leading to core collapse, expansion and eventual dispersion of every cluster. We review recent developments in the field with a focus on theoretical work.Peer reviewe

Cloaked websites: propaganda, cyber-racism and epistemology in the digital era

This article analyzes cloaked websites, which are sites published by individuals or groups who conceal authorship in order to disguise deliberately a hidden political agenda. Drawing on the insights of critical theory and the Frankfurt School, this article examines the way in which cloaked websites conceal a variety of political agendas from a range of perspectives. Of particular interest here are cloaked white supremacist sites that disguise cyber-racism. The use of cloaked websites to further political ends raises important questions about knowledge production and epistemology in the digital era. These cloaked sites emerge within a social and political context in which it is increasingly difficult to parse fact from propaganda, and this is a particularly pernicious feature when it comes to the cyber-racism of cloaked white supremacist sites. The article concludes by calling for the importance of critical, situated political thinking in the evaluation of cloaked websites

Vector Addition System Reachability Problem: A Short Self-contained Proof

International audienceThe reachability problem for Vector Addition Systems (VASs) is a central problem of net theory. The general problem is known to be decidable by algorithms exclusively based on the classical Kosaraju-Lambert-Mayr-Sacerdote-Tenney decomposition. This decomposition is used in this paper to prove that the Parikh images of languages recognized by VASs are semi-pseudo-linear; a class that extends the semi-linear sets, a.k.a. the sets definable in Presburger arithmetic. We provide an application of this result; we prove that a final configuration is not reachable from an initial one if and only if there exists a semi-linear inductive invariant that contains the initial configuration but not the final one. Since we can decide if a Presburger formula denotes an inductive invariant, we deduce that there exist checkable certificates of non-reachability. In particular, there exists a simple algorithm for deciding the general VAS reachability problem based on two semi-algorithms. A first one that tries to prove the reachability by enumerating finite sequences of actions and a second one that tries to prove the non-reachability by enumerating Presburger formulas

Quelques données concernant l'étude des minéraux argileux et des argiles en U.R.S.S.

Chukhrov F.V., Ginsburg I.I., Petrov V.P., Tsvetkov A.I., Gritsaenko G.S., Zvyagin B.B., Frank-Kamenetsky V.A., Popov Ivan Vasilevich. Quelques données concernant l'étude des minéraux argileux et des argiles en U.R.S.S.. In: Bulletin du Groupe français des argiles. Tome 12, nouvelle série n°7, 1960. pp. 43-61

Evaluation of family health history collection methods impact on data and risk assessment outcomes

10.1016/j.pmedr.2020.101072Preventive Medicine Reports1810107

Protocol for the "Implementation, adoption, and utility of family history in diverse care settings" study

10.1186/s13012-015-0352-8Implementation Science10116

Supplementary Material for: Impact of Delivery Models on Understanding Genomic Risk for Type 2 Diabetes

<b><i>Background:</i></b> Genetic information, typically communicated in-person by genetic counselors, can be challenging to comprehend; delivery of this information online - as is becoming more common - has the potential of increasing these challenges. <b><i>Methods:</i></b> To address the impact of the mode of delivery of genomic risk information, 300 individuals were recruited from the general public and randomized to receive genomic risk information for type 2 diabetes mellitus in-person from a board-certified genetic counselor or online through the testing company's website. <b><i>Results:</i></b> Participants were asked to indicate their genomic risk and overall lifetime risk as reported on their test report as well as to interpret their genomic risk (increased, decreased, or same as population). For each question, 59% of participants correctly indicated their risk. Participants who received their results in-person were more likely than those who reviewed their results on-line to correctly interpret their genomic risk (72 vs. 47%, p = 0.0002) and report their actual genomic risk (69 vs. 49%, p = 0.002). <b><i>Conclusions:</i></b> The delivery of personal genomic risk through a trained health professional resulted in significantly higher comprehension. Therefore, if the online delivery of genomic test results is to become more widespread, further evaluation of this method of communication may be needed to ensure the effective presentation of results to promote comprehension