10 research outputs found
Increased cerebrospinal fluid levels of cytokines monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-1β (MIP-1β) in patients with amyotrophic lateral sclerosis
Introduction: Neuroinflammation has recently been described in amyotrophic lateral sclerosis
(ALS). However, the precise role of such proinflammatory cytokines as monocyte chemoattractant
protein-1 (MCP-1) and macrophage inflammatory protein-1� (MIP-1�) in ALS has not yet
been determined. In this study, we determined cerebrospinal fluid (CSF) MCP-1 and MIP-1�
levels and assessed their association with the duration and severity of ALS.
Methods: Concentrations of MCP-1 and MIP-1� were determined in the CSF of 77 patients
diagnosed with ALS and 13 controls. Cytokine levels were analysed in relation to ALS duration
( 12 months) and severity ( 30 points on the ALS Functional Rating
Scale administered at hospital admission).
Results: Higher CSF MIP-1� (10.68 pg/mL vs 4.69 pg/mL, P < .0001) and MCP-1 (234.89 pg/mL
vs 160.95 pg/mL, P = .011) levels were found in the 77 patients with ALS compared to controls.
There were no differences in levels of either cytokine in relation to disease duration or severity.
However, we did observe a significant positive correlation between MIP-1� and MCP-1 in patients
with ALS.
Conclusions: The increase in MIP-1� and MCP-1 levels suggests that these cytokines may havea synergistic effect on ALS pathogenesis. However, in our cohort, no association was found witheither the duration or the clinical severity of the disease
Anormalidades clínicas y por resonancia magnética en lengua de pacientes con esclerosis lateral amiotrófica
Desarrollo de un medio de cultivo para la producción de polihidroxialcanoatos (PHA) por Brevibacterium halotolerans.
Incremento de las citoquinas proteína quimiotáctica de monocitos-1 (MCP-1) y proteína inflamatoria macrofágica-1β (MIP-1β) en líquido cefalorraquídeo de pacientes con esclerosis lateral amiotrófica
Introducción: En la esclerosis lateral amiotrófica (ELA) se ha descrito recientemente
la presencia de neuroinflamación. Sin embargo, no se ha definido el rol de citoquinas
proinflamatrorias como la proteína quimiotáctica de monocitos-1 (MCP-1) y
la proteína inflamatoria macrofágica-1� (MIP-1�) en ELA. En este estudio evaluamos
niveles de MCP-1 y MIP-1� en líquido cefalorraquídeo (LCR), analizando su participación en
la duración y gravedad de la ELA.
Métodos: En 77 pacientes con ELA definida y 13 sujetos controles, se comparó el nivel de
citoquinas MCP-1 y MIP-1� en LCR. Se analizaron estos niveles con relación a la duración de
la ELA ( 12 meses) y a la gravedad de esta determinada mediante el puntaje
obtenido al ingreso en la escala funcional estratificada de la ELA ( 30 puntos).
Resultados: En los 77 pacientes con ELA, se encontraron aumentados los niveles de MIP-1�
(4,69 pg/ml vs. 10,68 pg/ml, p < 0,0001) y MCP-1 (160,95 pg/ml vs. 234,89 pg/ml, p = 0,011) en
comparación con sujetos controles. No se observó diferencia de los niveles de estas citoquinas
con la duración o la gravedad de la enfermedad. Sin embargo, observamos una correlación
positiva significativa entre MIP-1� y MCP-1 en pacientes con ELA.
Conclusiones: El aumento de MIP-1� y MCP-1 sugiere que estas citoquinas parecen tener unefecto sinérgico en la patogénesis de la ELA. Sin embargo, en nuestra cohorte no se asociaroncon la duración o la gravedad de la ELA
Consenso Mexicano para el Tratamiento de la Hepatitis C
El objetivo del Consenso Mexicano para el Tratamiento de la Hepatitis C fue el de desarrollar un documento como guía en la práctica clínica con aplicabilidad en México. Se tomó en cuenta la opinión de expertos en el tema con especialidad en: gastroenterología, infectología y hepatología. Se realizó una revisión de la bibliografía en MEDLINE, EMBASE y CENTRAL mediante palabras claves referentes al tratamiento de la hepatitis C. Posteriormente se evaluó la calidad de la evidencia mediante el sistema GRADE y se redactaron enunciados, los cuales fueron sometidos a voto mediante un sistema modificado Delphi, y posteriormente se realizó revisión y corrección de los enunciados por un panel de 34 votantes. Finalmente se clasificó el nivel de acuerdo para cada oración. Esta guía busca dar recomendaciones con énfasis en los nuevos antivirales de acción directa y de esta manera facilitar su uso en la práctica clínica. Cada caso debe ser individualizado según sus comorbilidades y el manejo de estos pacientes siempre debe ser multidisciplinario.
Abstract
The aim of the Mexican Consensus on the Treatment of Hepatitis C was to develop clinical practice guidelines applicable to Mexico. The expert opinion of specialists in the following areas was taken into account: gastroenterology, infectious diseases, and hepatology. A search of the medical literature was carried out on the MEDLINE, EMBASE, and CENTRAL databases through keywords related to hepatitis C treatment. The quality of evidence was subsequently evaluated using the GRADE system and the consensus statements were formulated. The statements were then voted upon, using the modified Delphi system, and reviewed and corrected by a panel of 34 voting participants. Finally, the level of agreement was classified for each statement. The present guidelines provide recommendations with an emphasis on the new direct-acting antivirals, to facilitate their use in clinical practice. Each case must be individualized according to the comorbidities involved and patient management must always be multidisciplinary
TRY plant trait database – enhanced coverage and open access
Plant traits—the morphological, anatomical, physiological, biochemical and phenological characteristics of plants—determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait‐based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits—almost complete coverage for ‘plant growth form’. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait–environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives
Response surface methodology for lead biosorption on Aspergillus terreus
A central composite face-centered design was used to study and to
optimize lead biosorption from aqueous solution on Aspergillus terreus
biomass. Four factors such as stirring speed, temperature, solution pH
and biomass dose at different levels were studied.The hierarchical
quadratic model were established by adding replicates at the central
point and axial points to the initial full factorial design (24). The
percentage removal of lead was affected by biomass dose, pH, and
interactions between pH and biomass dose, pH and stirring speed, pH and
temperature. The hierarchical quadratic model described adequately the
response surface based on the adjusted determination coefficient
(R2Adj= 0.97) and the adequate precision ratio (42.21). According to
this model, the optimal conditions to remove lead completely from
aqueous solutions (at initial lead concentration of 50 mg/L and
solutions of 100 mL) with Aspergillus terreus were at pH 5.2,
50°C, stirring speed of 102/min and a biomass dose of 139 mg.The
response surface methodology can be used to determine the optimal
conditions for metal adsorption on several adsorbents. In addition,
results reported in this research demonstrated the feasibility of
employing A. terreus as biosorbent for lead removal
Biosorption of Cu(II) and Pb(II) from aqueous solutions by chemically modified spent coffee grains
In this research, spent coffee grains were modified with citric acid
solutions (0.1 and 0.6 M) to increase the quantity of carboxylic groups
improving its metal adsorption capacity. Added functional groups on
modified and non-modified spent coffee grains were identified and
quantified by attenuated total reflection Fourier transform infrared
analyses and potentiometric titrations, respectively. These adsorbents
were used for the removal of lead (II) and copper (II) from aqueous
solutions at 30 °C and different pH in batch systems. In addition,
adsorption–desorption experiments were conducted to evaluate the
possibility of re-using the modified adsorbent. Potentiometric
titrations data reveal that the quantity of carboxylic groups was
increased from 0.47 to 2.2 mmol/g when spent coffee grains were
modified with 0.1 and 0.6 M citric acid. Spent coffee grains treated
with 0.6 M citric acid, achieved a maximum adsorption capacity of 0.77
and 1.53 mmol/g for lead (II) and copper (II), respectively, whereas
non-modified spent coffee grains only reached 0.24 and 0.19 mmol/g for
lead (II) and copper (II), respectively. Desorption of lead (II) and
copper (II) achieved around 70 % using 0.1 N HCl for non-modified and
modified spent coffee grains with 0.6 M citric acid. It is suggested
that lead (II) and copper (II) species were adsorbed mainly on the
carboxylic groups of modified spent coffee grains and these metals may
be exchanged for hydrogen and calcium (II) ions during adsorption on
non-modified spent coffee grains. Finally, the adsorption equilibrium
was reached after 400 min for modified spent coffee grains with 0.6 M
citric acid. Modified spent coffee grains are a promising option for
removing metal cations from aqueous solutions due to its low cost and
high adsorption capacity (about 10 times higher than the activated
carbons)
30-day morbidity and mortality of sleeve gastrectomy, Roux-en-Y gastric bypass and one anastomosis gastric bypass: a propensity score-matched analysis of the GENEVA data
Background: There is a paucity of data comparing 30-day morbidity and mortality of sleeve gastrectomy (SG), Roux-en-Y gastric bypass (RYGB), and one anastomosis gastric bypass (OAGB). This study aimed to compare the 30-day safety of SG, RYGB, and OAGB in propensity score-matched cohorts. Materials and methods: This analysis utilised data collected from the GENEVA study which was a multicentre observational cohort study of bariatric and metabolic surgery (BMS) in 185 centres across 42 countries between 01/05/2022 and 31/10/2020 during the Coronavirus Disease-2019 (COVID-19) pandemic. 30-day complications were categorised according to the Clavien–Dindo classification. Patients receiving SG, RYGB, or OAGB were propensity-matched according to baseline characteristics and 30-day complications were compared between groups. Results: In total, 6770 patients (SG 3983; OAGB 702; RYGB 2085) were included in this analysis. Prior to matching, RYGB was associated with highest 30-day complication rate (SG 5.8%; OAGB 7.5%; RYGB 8.0% (p = 0.006)). On multivariate regression modelling, Insulin-dependent type 2 diabetes mellitus and hypercholesterolaemia were associated with increased 30-day complications. Being a non-smoker was associated with reduced complication rates. When compared to SG as a reference category, RYGB, but not OAGB, was associated with an increased rate of 30-day complications. A total of 702 pairs of SG and OAGB were propensity score-matched. The complication rate in the SG group was 7.3% (n = 51) as compared to 7.5% (n = 53) in the OAGB group (p = 0.68). Similarly, 2085 pairs of SG and RYGB were propensity score-matched. The complication rate in the SG group was 6.1% (n = 127) as compared to 7.9% (n = 166) in the RYGB group (p = 0.09). And, 702 pairs of OAGB and RYGB were matched. The complication rate in both groups was the same at 7.5 % (n = 53; p = 0.07). Conclusions: This global study found no significant difference in the 30-day morbidity and mortality of SG, RYGB, and OAGB in propensity score-matched cohorts. © 2021, The Author(s)
TRY plant trait database, enhanced coverage and open access
Plant traits-the morphological, ahawnatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives