627 research outputs found

    Using Single Molecule Imaging to Explore Intracellular Heterogeneity

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    Despite more than 100 years of study, it is unclear if the movement of proteins inside the cell is best described as a mosh pit or an exquisitely choreographed dance. Recent studies suggest the latter. Local interactions induce molecular condensates such as liquid-liquid phase separations (LLPSs) or non-liquid, functionally significant molecular aggregates, including synaptic densities, nucleoli, and Amyloid fibrils. Molecular condensates trigger intracellular signaling and drive processes ranging from gene expression to cell division. However, the descriptions of condensates tend to be qualitative and correlative. Here, we indicate how single-molecule imaging and analyses can be applied to quantify condensates. We discuss the pros and cons of different techniques for measuring differences between transient molecular behaviors inside and outside condensates. Finally, we offer suggestions for how imaging and analyses from different time and space regimes can be combined to identify molecular behaviors indicative of condensates within the dynamic high-density intracellular environment.Comment: 21 pages, 2 figures, 1 tabl

    The Big Deal Is Dead! Long Live The Big Deal!

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    In many countries, the proclamation ā€œThe King is dead, long live the Kingā€ heralds the demise of the old monarch and the accession of a new one. This tradition ensures that the throne never remains empty while facilitating a smooth transition of power. When the ā€œBig Dealā€ journal subscription model debuted in 1996, few suspected the extent to which academic libraries would come to rely upon it, or that it would become the primary channel by which academic libraries procure academic journal content. As budget cuts take their toll on libraries, the demise of the Big Deal model seems inevitable as the true value of all-inclusive packages becomes less evident. But is it? Collection analysis reveals that many titles included within these ā€œBig Dealā€ packages remain unused or underutilized, significantly decreasing the overall value of serial subscription packages. SPARCā€™s Big Deal Cancellation Tracker shows an increasing number of libraries and consortia forgoing this model in favor of regaining local control over their collections and budgets. The Binghamton University Libraries is no exception. Recent curriculum changes and financial developments have prompted us to adopt an ongoing evaluation of our usersā€™ information needs and proactively negotiate and cancel deals in order to better serve our constituents. This session described our fact finding, workflow modifications, and data analysis processes as well as the outcomes of our adventures in pursuing and planning for the cancellation of Big Deal agreements based on local collection development priorities and serials budget realities

    Modeling Intermittent Running from a Single-visit Field Test

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    This study assessed whether the distance-time relationship could be modeled to predict time to exhaustion (TTE) during intermittent running. 13 male distance runners (age: 33 Ā± 14 years) completed a field test and 3 interval tests on an outdoor 400 m athletic track. Field-tests involved trials over 3600 m, 2400 m and 1200 m with a 30-min rest between each run. Interval tests consisted of: 1000 m at 107 % of CS with 200 m at 95 % CS; 600 m at 110 % of CS with 200 m at 90 % CS; 200 m at 150 % of CS with 200 m at 80 % CS. Interval sessions were separated by 24 h recovery. Field-test CS and Dā€² were applied to linear and non-linear models to estimate the point of interval session termination. Actual and predicted TTE using the linear model were not significantly different in the 1000 m and 600 m trials. Actual TTE was significantly lower (P = 0.01) than predicted TTE in the 200 m trial. Typical error was high across the trials (range 334ā€“1709 s). The mean balance of Dā€² remaining at interval session termination was significantly lower when estimated from the non-linear model (āˆ’ 21.2 vs. 13.4 m, P < 0.01), however no closer to zero than the linear model. Neither the linear or non-linear model could closely predict TTE during intermittent running

    A Single-Visit Field Test of Critical Speed

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    Purpose: To compare critical speed (CS) measured from a single-visit field test of the distanceā€“time relationship with the ā€œtraditionalā€ treadmill time-to-exhaustion multivisit protocol. Methods: Ten male distance runners completed treadmill and field tests to calculate CS and the maximum distance performed above CS (Dā€²). The field test involved 3 runs on a single visit to an outdoor athletics track over 3600, 2400, and 1200 m. Two field-test protocols were evaluated using either a 30-min recovery or a 60-min recovery between runs. The treadmill test involved runs to exhaustion at 100%, 105%, and 110% of velocity at VO2max, with 24 h recovery between runs. Results: There was no difference in CS measured with the treadmill and 30-min- and 60-minrecovery field tests (P .05). A typical error of the estimate of 0.14 m/s (95% confidence limits 0.09ā€“0.26 m/s) was seen for CS and 88 m (95% confidence limits 60ā€“169 m) for Dā€². A coefficient of variation of 0.4% (95% confidence limits: 0.3ā€“0.8%) was found for repeat tests of CS and 13% (95% confidence limits 10ā€“27%) for Dā€². Conclusion: The single-visit method provides a useful alternative for assessing CS in the field

    Taxonomic voucher specimens for study of bee communities in intensively managed Douglas-fir forests in the Oregon Coast Range

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    Understanding how pollinators respond to anthropogenic land use is key to conservation of biodiversity and ecosystem services, but few studies have addressed this topic in coniferous forests, particularly those managed intensively for wood production. This study reports on voucher material generated as part of Zitomer et al. (2023), that assessed changes in wild bee communities with time since harvest in 60 intensively managed Douglas-fir (Pseudotsuga menziesii) stands in the Oregon Coast Range across a gradient in stand age spanning a typical harvest rotation (0-37 years post-harvest). We additionally assessed relationships of bee diversity and community composition to relevant habitat features, including availability of floral resources and nest sites, understory vegetation characteristics, and composition of the surrounding landscape. Specimens were collected using a combination of passive sampling methods-blue vane traps and white, blue, and yellow bowl traps- and hand-netting and were identified to the lowest possible taxonomic level by A.R. Moldenke and L.R. Best. Four hundred and ten taxonomic voucher specimens were deposited into the Oregon State Arthropod Collection (Accession# OSAC_AC_2023_01_09-001-01) to serve as a reference for future research

    Stratus 13 thirteenth setting of the Stratus Ocean Reference Station cruise on board RV Ron Brown February 25 - March 15, 2014 Valparaiso, Chile - Arica, Chile

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    The Ocean Reference Station at 20Ā°S, 85Ā°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology, air-sea fluxes of heat, freshwater, and momentum, and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administrationā€™s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with past cruises that have come between October and January. This cruise was conducted on the NOAA vessel Ron Brown. During the 2014 cruise on the Ron Brown to the ORS Stratus site, the primary activities were the recovery of the previous (Stratus 12) WHOI surface mooring, which was adrift since January 25 2014 and drifting northwest, deployment of the new Stratus 13 WHOI surface mooring, in-situ calibration of the buoy meteorological sensors by comparison with instrumentation installed on the ship, CTD casts near the moorings. Surface drifters and subsurface floats were also launched along the track.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA09OAR4320129 and the Cooperative Institute for the North Atlantic Region (CINAR)

    Stratus 11 : Eleventh Setting of the Stratus Ocean Reference Station Cruise on board RV Moana Wave, March 31 - April 16, 2011, Arica - Arica, Chile

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    The Ocean Reference Station at 20Ā°S, 85Ā°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology, air-sea fluxes of heat, freshwater, and momentum, and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administrationā€™s (NOAA) Climate Observation Program. It is recovered and redeployed annually, with past cruises that have come between October and January. A NOAA vessel was not available, so this cruise was conducted on the chartered ship, Moana Wave, belonging to Stabbert Maritime. During the 2011 cruise on the Moana Wave to the ORS Stratus site, the primary activities were the recovery of the subsurface part of the Stratus 10 WHOI surface mooring, deployment of a new (Stratus 11) WHOI surface mooring, in-situ calibration of the buoy meteorological sensors by comparison with instrumentation installed on the ship by staff of the NOAA Earth System Research Laboratory (ESRL), and collection of underway and on station oceanographic data to continue to characterize the upper ocean in the stratus region. The Stratus 10 mooring had parted, and the surface buoy and upper part had been recovered earlier. Underway CTD (UCTD) profiles were collected along the track and during surveys dedicated to investigating eddy variability in the region. Surface drifters and subsurface floats were also launched along the track. The intent was also to visit a buoy for the Pacific tsunami warning system maintained by the Hydrographic and Oceanographic Service of the Chilean Navy (SHOA). This DART (Deep- Ocean Assessment and Reporting of Tsunami) buoy had been deployed in December 2010.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA0900AR432012

    Stratus 12 : twelfth setting of the Stratus Ocean Reference Station

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    The Ocean Reference Station at 20Ā°S, 85Ā°W under the stratus clouds west of northern Chile is being maintained to provide ongoing climate-quality records of surface meteorology, air-sea fluxes of heat, freshwater, and momentum, and of upper ocean temperature, salinity, and velocity variability. The Stratus Ocean Reference Station (ORS Stratus) is supported by the National Oceanic and Atmospheric Administrationā€™s (NOAA) Climate Observation Program. It is recovered and redeployed annually. A NOAA vessel was not available, so this cruise was conducted on the Melville, operated by the Scripps Institution of Oceanography. During the 2012 cruise on the Melville to the ORS Stratus site, the primary activities were the deployment of the Stratus 12 WHOI surface mooring, recovery of the previous (Stratus 11) WHOI surface mooring, in-situ calibration of the buoy meteorological sensors by comparison with instrumentation installed on the ship, and collection of underway and on station oceanographic data to continue to characterize the upper ocean in the stratus region. Underway CTD (UCTD) profiles were collected along the track. Surface drifters and subsurface floats were also launched along the track.Funding was provided by the National Oceanic and Atmospheric Administration under Grant No. NA09OAR4320129
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