Amplifikasi DNA Kandidat Gen Kuda Pacu Sumba

The Sumba horse is one of the local horses in Indonesia which is known as racing horse Several candidate genes are known to influence the outward characteristics of the Sumba racehorse, which play main role in the development of the horse's muscles from embryo to adulthood. This research aims to identify candidate genes for the Sumba racehorse in stallion and mares. Blood samples from 5 stallions and 5 mares were collected and analyzed. The method used in this research was by using polymerase chain reaction (PCR), electrophoresis and DNA sequencing. The results of DNA amplification fragments at a temperature of 600c showed a fragment size of 463 bp. A total of 10 samples were sequenced on the PCR machine. The forward primer was 5'-TATTCTTCTTGGGAGGGAGGACTACT-3 'and reverse primer was 5'-GCAAGTAATTAGCACAAAAATTTGAATG-3'. The obtained data was analyzed using the Basic Local Alignment Sealing Tool (BLAST). Result of this study could be used as an initial identification of candidate genes for racing activity in stallion and mares that can complement the selection of racing horses

FA-7 Practical Applications of Ultrasound for Pregnancy Diagnosis in Bali Cattle Herded Semi-Intensively in Maumere, NTT

Generally, real time, B-mode ultrasound scanner has become an essential part for veterinary reproduction applications. Diagnostic ultrasound seems to be a useful tool to study anatomical structures and to confirm echogenic pattern in reproductive organ (Holman et al, 2011). Many experiments showed that ultrasonography imaging has considerable beneficial for the evaluation of the internal structure of reproductive organ function in domestic animals (Beal et al, 1992) as it can be used as a non-invasive technique to evaluate animal reproductive health (Holman et al 2011). Pregnancy detection with ultrasonography provides more advantage compare to manual palpation because of its ability to detect early presence of embryo and its accuracy (Beal et al, 1992; Nation et al 2003). To the best of our knowledge, most of cattle farmers and veterinarians in Maumere have relied on one single method for detecting pregnancy in cows, that is, rectal palpation. However, this method has its limitation as it should be performed by a skillful technician to diagnose pregnancy as early as 40 days of gestation and it does not provide any information about the viability of the embryo or fetus. Therefore, this study aims to investigate pregnancy status of Bali cattle herded semi-intensively in Maumere, NTT by using ultrasonography.The objective of the research was to study the practical uses of ultrasound for pregnancy detection in Bali cattle on B-mode ultrasound imaging

Studi Performa Umum Tubuh dan Status Fisiologis Kuda Sumba

Penelitian mengenai studi performa umum dan status fisiologis kuda Sumba di dilaksanakan pada tanggal April-September 2018 bertempat di Peternakan kuda sumba, Pulau Sumba, NTT. Tujuan penelitian ini adalah untuk mengetahui performa umum kuda sumba dalam kaitannya dengan status fisiologisnya. Penelitian yang digunakan adalah metode deskriptif yaitu metode yang menggambarkan objek sesuai dengan apa adanya, pengambilan sampel dilakukan selama dua minggu. Dalam penelitian ini peubah yang diamati adalah frekuensi respirasi, frekuensi denyut jantung dan suhu tubuh yang dilakukan pada 30 ekor indukan kuda sumba memiliki riwayat pernah digunakan untuk olahraga kuda pacuan baik kuda dengan jenis kelamin jantan dan betina dan 5 ekor anakan. Hasil dari penelitian tersebut disimpulkan bahwa frekuensi respirasi indukan memiliki nilai rataan 33±6.7 per menit dan anak memiliki nilai rataan 44±5.7 per menit. Frekuensi denyut jantung indukan memiliki nilai rataan 44±8.0 per menit dan 62±2.8 per menit . Suhu tubuh kuda indukan memiliki rataan yaitu 37.5±0.60C dan suhu anak memiliki rataan yaitu 38.2 ± 0.30C. Selain itu, hasil penelitian menunjukkan bahwa kuda indukan memiliki rataan tinggi badan 138.8 ±8.4 cm dan anakan memiliki rataan 106.2 ± 7.1 cm; indukan memiliki rataan panjang badan 139.4 ± 9.0 cm dan anakan rataan 94.4 ± 3.0; indukan memiliki rataan lebar dada 41.3  ±57.1 cm dan anakan memiliki rataan lebar dada 21.2 ± 3.1 cm; indukan memiliki tinggi punggung 133.9 ± 8.8 cm dan anak memiliki rataan tinggi punggung 104.4 ± 8.6 cm dan indukan memiliki panjang bahu 58.5 ± 5.5 cm dan anak memiliki rataan panjang bahu 40.0 ±5.6 cm                                                                                                                                         &nbsp

Pola Perkawinan dan Estimasi Kelahiran Berdasarkan Hasil Diagnosis Kebuntingan Sapi Bali pada Sistem Pemeliharaan Semi Intensif di Daerah Lahan Kering

Pola perkawinan dan kelahiran menjadi salah satu faktor penting dari manajemen reproduksi pada sistem pemeliharan semi intensif. Penelitian ini dilakukan untuk mengetahui pola perkawinan dan estimasi kelahiran berdasarkan hasil diagnosis kebuntingan sapi bali pada sistem pemeliharaan semi intensif padang penggembalaan di daerah lahan kering. Manfaat dari penelitian ini adalah sebagai data base dalam penerapan manajemen perkawinan. Sebanyak 208 ekor induk sapi bali di kelompok ternak Tamnau Amaf, Desa Bena, Kecamatan Amanuban Selatan, Kabupaten Timor Tengah Selatan, Provinsi NTT dilakukan anamnesa dan pemeriksaan per rektal pada tanggal 28-30 Mei 2019. Angka kebuntingan yang diketahui pada penelitian ini adalah 90.86 % (189/208). Persentase usia kebuntingan tertua adalah usia kebuntingan 7 bulan 26,96 % (56/208). Berdasarkan data kebuntingan diketahui puncak pola perkawinan terjadi pada bulan Oktober 2018 dan estimasi kelahiran mencapai puncak pada bulan Juli 2019. Perkawinan sapi sebaiknya dilakukan pada kurun waktu bulan Januari - Maret sehingga kelahiran terjadi pada kurun waktu bulan Oktober - Desember. Perlu perbaikan manajemen perkawinan sapi bali pada sistem pemeliharaan semi intensif padang penggembalaan di daerah lahan kering sehingga kelahiran tidak terjadi pada puncak musim kemarau

TINJAUAN HASIL INSEMINASI BUATAN SAPI BALI DI DESA PUKDALE KECAMATAN KUPANG TIMUR KABUPATEN KUPANG

Bali Cattle is one of regional economic support for East Nusa Tenggara (NTT). Bali Cattle has a particularly important role for the that is as the supplier of animal protein, a source of income, savings and field works. Bali cattle most popular in NTT particular in Village Pukdale East kupang sub- District Kupang Regency. The purpose of this study is to find Service per conception (S/C), Pregnancy Rate, Days Open (DO) and Calving Interval (CI) of Bali cattle be inseminated in village Pukdale East Kupang Sub- District Kupang Regency. The number of cattles used in this study were 30 cows. This research used female Bali cattle at least two times calve, age 4-7 years, Body Condition Score (BCS) 3-2 from standard 5-1 and has not disorder reproduction .The research results show that average score Service per conception (S/C) 2,33, Pregnancy Rate 60 %, Days Open 102,3 days and Calving Intervals (CI) 13,019 months. The results of the study showing that the Pregnancy Rate, Days Open (DO) and Calving Intervals (CI) is in the range of normal while value Service per conception (S/C) unwell so it needs of an improvement on the implementation of the program artificial insemination in the village pukdale covering the ability farmers in detecting estrus and the availability of nitrogen (N2) liqui

PENAMPILAN REPRODUKSI SAPI BALI PADA SISTEM PEMELIHARAAN SEMI INTENSIF

Bali cattle is one of the native cattle in Indonesia. High fertility and are capabled to adapt well in tropical environment is one of the superiority of bali cattle. Bali cattle farming in East Nusa Tenggara (NTT) are generally managed by intensive, semi-intensive and extensive system traditionaly, without any recording and lacking good breeding practices. The purpose of this research is to determine the reproduction performance of bali cattle on semi-intensive management system based on pregnancy rate, days open and calving interval. The number of cattles used in this study were 40 cows on age 4-7 years. The methods used were interviews and rectal palpation to know the reproductive status of livestock. Descriptive analysis was used in this research. The results showed that the pregnancy rate was 45%, days open was 112,5 days and calving interval was 13,075 months. This study indicated a decreased reproductive efficiency of bali cattle in Kupang Regency, hence an improvement of the management reproduction is required to achieve good reproductive management

GAMBARAN HISTOPATOLOGI HEPAR TIKUS PUTIH (Rattus norvegicus) JANTAN PASCA PEMBERIAN EKSTRAK INFUSA BUAH PARE (Momordica charantia L.) LOKAL NTT

Hepatic is the largest gland in the body. Hepatic function in the metabolism and detoxification of a wide variety of compounds into the body. Infusion extract of bitter melon (M. charantia L.) fruit is a herbal preparation containing chemical compounds such as flavonoids, glycosides, triterpenoids, polyphenols, alkaloids, saponins, and tannins. Excessive drug dosing in a long period of time, can make liver damage acute, subacute or chronic. Toxic effects of drugs often seen in the tissues, especially the liver and kidneys are on histology, appearing as inflammatory cell infiltration, hidropic degeneration, fatty degeneration and necrosis. The purpose of this study is to determine the effect of variations in dose infusion extract of bitter melon fruit (M. charantia L.) locally NTT against white picture rat liver histopathology (R. norvegicus) male and determine the effective dose infusion extract of bitter melon fruit (M. charantia L.) NTT local who gave a response, or effects on fertility drugs white rat (R. norvegicus) male. The method used in this study is completely randomized design method namely by dividing the mice randomized into two treatment groups with different doses variations. Analysis of the data used is descriptive. The results obtained showed that the average infiltration in P1 was 5.8 ± 6.7, while the P2 is 3.7 ± 5.5. Hydropic degeneration in P1 was 17.3 ± 10.7 and in P2 was 63.5 ±32.7. fatty degeneration in P1 was 2.3 ± 4.4 and 10.2 ± At P2 is 9.5. Necrosis of P1 was 0.5 ± 0.8, while the P2 is 4.2 ± 2.9

GAMBARAN HISTOPATOLOGI GINJAL TIKUS PUTIH (Rattus norvegicus) JANTAN YANG DIBERI INFUSA PARE LOKAL PULAU TIMOR

Some of the chemical compounds in bitter melon fruit that acts as infertility for males, namely saponins, tannins, alkaloids, flavonoids and triterpenoids were able to reduce the number of spermatogenic cells. Renal excretory function in carrying out this gets tough task, because almost 25% of all blood flow to the two kidneys. The amount of blood flow to the kidney causes renal exposure to the material circulating in the circulation system is quite high, so that the toxic material will easily cause damage to the kidney tissue. This study aimed to determine the effect of dose delivery infusion of local bitter melon fruit on the island of Timor, East Nusa Tenggara against renal histopathology description of male white rat (Rattus norvegicus). The study was conducted in the 2 treatment groups, namely (P1) bitter melon infusion at a dose of 1250 mg/kgBB/day and (P2) bitter melon infusion at a dose of 2500 mg/kgBB/day. Each treatment group consisted of 6 rats administered for 48 days. Microscopic changes that occur in the kidneys of mice include glomerular congestion, atrophy of the glomerulus, tubular hemorrhage, necrosis of the tubules are composed of cells piknosis, Karyorrhexis cells, karyolysis cells and tubular protein deposits. The average glomerular damage and kidney tubules was higher in treatment P2 than P1 which indicates the ravages caused by high dose bitter melon infusion given to the treatment group P1 and P2 so that the chemical substances to be excessive and toxic, accumulate in the kidneys and cause damage to the kidney

KUALITAS SPERMATOZOA BABI LANDRACE YANG DIBERI EKSTRAK DAUN KELOR (Moringa oleifera Lam.) SEBAGAI ANTIOKSIDAN PADA SUHU PENYIMPANAN BERBEDA

This study aims to determine the quality of Landrace porkspermatozoa with the addition of natural thinners of lontar fruit juice and Moringa oleifera Lam. Leaf extract as antioxidants stored at different storage temperatures, namely 5°C (freezer),15°C (refrigerator) and 22°C (air-conditioned room). Semen was collected six times and evaluated both macroscopically and microscopically. The best quality of semen was semen with spermatozoa motility> 70%, concentration> 200x106 cells / ml and abnormal spermatozoa <20%. In this study, there were three treatments, in which the cement with antioxidant diluents was stored at 15°C. (treatment I), 22°C (treatment II) and 5 oC (treatment III). The results showed at the 36th hour of storage the temperature was 22°C with a motility of 55.00 ± 0.20% and a viability of 72.50 ± 0.27%, a temperature of 15°C with a motility of 55.00 ± 0.28% and a viability of 64.50 ± 0.20%, and a temperature of 5°C with a motility of 50.00 ± 0.50 % and viability 57.50 ± 0.33%. It was concluded that the water thinner of lontar fruit and the antioxidant of Moringa oleifera Lam. Leaf extract were able to maintain the quality of the liquid Landrace pig semen at different storage temperatures. A significant difference (P≤0.05) occurred between the 5°C and 22°C treatment, while the 15°C temperature was not significantly different (P≥0.05) with the 5°C and 22°C temperatures