Archaeobotanical interpretation of a Middle Bronze Age (1914-1641 cal BC) cave site in south-western Sardinia (Italy)

The Monte Meana karst cave is located in the Sulcis region, 5 km far from Santadi (SW Sardinia, Italy). The cave opens at 168 meters on the south-western side of Meana Mt. and the Murrecci river flows below. From the Middle Neolithic to the Middle Bronze age the cave has been inhabited. In the 1960s an alabaster quarry destroyed several archaeological strata. In the northern side of the cave, a small opening, here called ‘grottino’, was discovered under 1.50 m. of quarry's debris. Within it, an undamaged stratigraphy 1 m. depth and a sequence of four layers has been excavated in a 4mq area. At the top, a sandy layer, originated by quarrying activity, was also identified. Below, a dark brown deposit of 15-20 cm of thickness, characterized by several archaeological remains as pottery fragments, obsidian, copper slags, burnt bones, shells and a large amount of charcoals and carbonized seeds, has been investigated as well. In the underlying layers no archaeological remains have been found. The dark brown deposit (SU22) was protected and sealed by a recent sandy layer thus its sediment was uncontaminated. This fact has represented a lucky opportunity to identify some palaeoenvironmental features. Therefore, palynological and carpological analysis and characterization of palynofacies have been performed. The results bear out the presence of an open area around the site that included herbaceous plants and shrublands. Different agriculture practices as cereals and legumes cultivations are attested as well. The limited presence of pollen grains that are representative of arboreal plants during Middle Bronze Age is probably climate induced and linked to anthropic activities: for example, deforestation, burning for the cultivation of cereal crops, and the development of grazing areas

Predictive method for correct identification of archaeological charred grape seeds: Support for advances in knowledge of grape domestication process

The identification of archaeological charred grape seeds is a difficult task due to the alteration of the morphological seeds shape. In archaeobotanical studies, for the correct discrimination between Vitis vinifera subsp. sylvestris and Vitis vinifera subsp. vinifera grape seeds it is very important to understand the history and origin of the domesticated grapevine. In this work, different carbonisation experiments were carried out using a hearth to reproduce the same burning conditions that occurred in archaeological contexts. In addition, several carbonisation trials on modern wild and cultivated grape seeds were performed using a muffle furnace. For comparison with archaeological materials, modern grape seed samples were obtained using seven different temperatures of carbonisation ranging between 180 and 340°C for 120 min. Analysing the grape seed size and shape by computer vision techniques, and applying the stepwise linear discriminant analysis (LDA) method, discrimination of the wild from the cultivated charred grape seeds was possible. An overall correct classification of 93.3% was achieved. Applying the same statistical procedure to compare modern charred with archaeological grape seeds, found in Sardinia and dating back to the Early Bronze Age (2017-1751 2σ cal. BC), allowed 75.0% of the cases to be identified as wild grape. The proposed method proved to be a useful and effective procedure in identifying, with high accuracy, the charred grape seeds found in archaeological sites. Moreover, it may be considered valid support for advances in the knowledge and comprehension of viticulture adoption and the grape domestication process. The same methodology may also be successful when applied to other plant remains, and provide important information about the history of domesticated plant

Dental pathology in present-day and copper age samples

Dental paleopathology has become an excellent discipline to reconstruct the oral health of ancient populations and its trend from the past to the present day, especially regarding dietary habits. Our preliminary research aims to perform a comparative analysis on dental health status of two widely chronologically distant samples from Sardinia: the first one dates back to the Copper Age (III mill. B.C.) and comes from a collective hypogean burial named Scab’e Arriu (Siddi, SU), the second one is composed by extracted teeth of present-day individuals, collected during some traineeships at the Department of Surgical Science of the Dentistry School, in Cagliari

Proteasome stress sensitizes malignant pleural mesothelioma cells to bortezomib-induced apoptosis

Abstract Based on promising results in preclinical models, clinical trials have been performed to evaluate the efficacy of the first-in-class proteasome inhibitor bortezomib towards malignant pleural mesothelioma (MPM), an aggressive cancer arising from the mesothelium of the serous cavities following exposure to asbestos. Unexpectedly, only minimal therapeutic benefits were observed, thus implicating that MPM harbors inherent resistance mechanisms. Identifying the molecular bases of this primary resistance is crucial to develop novel pharmacologic strategies aimed at increasing the vulnerability of MPM to bortezomib. Therefore, we assessed a panel of four human MPM lines with different sensitivity to bortezomib, for functional proteasome activity and levels of free and polymerized ubiquitin. We found that highly sensitive MPM lines display lower proteasome activity than more bortezomib-resistant clones, suggesting that reduced proteasomal capacity might contribute to the intrinsic susceptibility of mesothelioma cells to proteasome inhibitors-induced apoptosis. Moreover, MPM equipped with fewer active proteasomes accumulated polyubiquitinated proteins, at the expense of free ubiquitin, a condition known as proteasome stress, which lowers the cellular apoptotic threshold and sensitizes mesothelioma cells to bortezomib-induced toxicity as shown herein. Taken together, our data suggest that an unfavorable load-versus-capacity balance represents a critical determinant of primary apoptotic sensitivity to bortezomib in MPM

Application of latent class analysis in assessing the awareness, attitude, practice and satisfaction of paediatricians on sleep disorder management in children in Italy.

AIM: To identify subgroups regarding paediatricians' awareness, attitude, practice and satisfaction about management of Sleep-Disordered Breathing (SDB) in Italy using Latent Class Analysis (LCA). METHODS: A cross-sectional study was conducted on a large sample of Italian paediatricians. Using a self-administered questionnaire, the study collected information on 420 Paediatric Hospital Paediatricians (PHPs) and 594 Family Care Paediatricians (FCPs). LCA was used to discover underlying response patterns, thus allowing identification of respondent groups with similar awareness, attitude, practice and satisfaction. A logistic regression model was used to investigate which independent variables influenced latent class membership. Analyses were performed using R 3.5.2 software. A p-value<0.05 was considered statistically significant. RESULTS: Two classes were identified: Class 1 (n = 368, 36.29%) "Untrained and poorly satisfied" and Class 2 (n = 646, 63.71%) "Trained and satisfied." Involving paediatric pneumologists or otorhinolaryngologists in clinical practice was associated with an increased probability of Class 2 membership (OR = 5.88, 95%CI [2.94-13.19]; OR = 15.95, 95% CI [10.92-23.81] respectively). Examining more than 20 children with SDB during the last month decreased the probability of Class 2 membership (OR = 0.29, 95% CI [0.14-0.61]). FCPs showed a higher probability of Class 2 membership than PHPs (OR = 4.64, 95% CI [3.31-6.55]). CONCLUSIONS: These findings suggest that the LCA approach can provide important information on how education and training could be tailored for different subgroups of paediatricians. In Italy standardized educational interventions improving paediatricians' screening of SDB are needed in order to guarantee efficient management of children with SDB and reduce the burden of disease

Evaluation of an automatic HPLC analyser for thalassemia and haemoglobin variants screening

In this paper the authors report the evolution of a new automatic HPLC analyser for screening haemoglobinopathies. HbA2 and F determinations are accurate and reproducible. The analysis time is short (6.5 min) and there is a good separation between the HbA2 values of β-thalassemia carriers from normals and α-thalassemia carriers, with no overlap between these groups. In addition, the system is also able to detect and quantitate most of the haemoglobin variants, particularly those (HbS, HbC, HbE and Hb Lepore) able to interact with β-thalassemia and could make haemoglobin electrophoresis unnecessary in all samples. The ease of operation and the limited technical work make this system especially suitable for laboratories with a high workload and allow the cost of screening to be reduced

Mesonephric-Like Adenocarcinomas a Rare Tumor: The Importance of Diagnosis

Mesonephric-like adenocarcinomas (MLA) are rare neoplasms that arise in the uterine body and ovary and have been added to the World Health Organisation’s recent 2020 classification of female genital cancers. The pathogenesis of MLA is unknown and it remains debated whether they represent mesonephric carcinomas (Wolffian) arising in the endometrium/ovary or endometrioid carcinomas (Müllerian) closely mimicking mesonephric carcinomas. Here we report the case of a 57-year-old woman with an initial misdiagnosis of endometrioid adenocarcinoma on diagnostic biopsy. The patient came to our clinical evaluation for the appearance of menometrorrhagia complicated by anemia for several months. Therefore, she underwent pelvic echo-flowmetry, with indication for diagnostic hysteroscopy with endometrial biopsy, which yielded a positive result for endometrioid endometrial adenocarcinoma. Following staging CT scan and targeted examinations on pulmonary findings, the patient underwent surgery with surprise of definitive diagnosis deponent for endometrial MLA. Our intention is to establish a brief review of the scientific evidence in the literature and the tools available for a correct histological diagnosis, in the light of the scant anatomopathological evidence. Our question gives rise to the motive for the publication: is immunohistochemistry the right way to resolve the diagnostic error at histology, which is usually the only source of diagnostic certainty? This case is intended to alert of diagnostic error that risked having the patient treated as a neoplasm with a favorable prognosis and low degree of aggressiveness instead of for a very aggressive and poor prognosis tumor such as MLA

Le rotonde con bacile d'età nuragica

Roundhouses whit basin of Nuragic Age - Some Nuragic settlements which date back to the period of time comprised between the Last Bronze Age and the First Iron Age have some rooms at their inside, which are called ”roundhouses.” They are small circular rooms with seats and additional elements, such as round and rectangular basins and fireplaces. The present work offers a survey of known cases through the comparison of their peculiar context, dimensional data, and workmanship, which suggest the existence of a model which is still used today

Da Barumini a Lipari. Due contesti del Bronzo finale a confronto

SuMMaRy - From Barumini to Lipari - Two Late Bronze age contexts in comparison - In 1980 L. Bernabò Brea and M. Cavalier published the results of excavation of the prehistoric settlement in Lipari’s Castle. In particular, in the excavation of hut alpha II the archaeologists found nuragic pottery in the Late Bronze age level of occupation. Two important papers were recently published that offered a new critical analysis of this nuragic pottery found in the ausonio II layers of the Lipari’s Castle. These papers are very important from a technological, chronological and stratigraphic position point of view and have led to a better understanding of manufacturing process of that period. This has resulted in new research on the pottery found in the huts of the nuragic complex of Su Nuraxi in Sardinia. In this context there are vessels decorated in a protogeometric style similar to those found in Lipari. These similarities show that the pottery originates from the protogeometrical phase of the Late Bronze age