Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: Functional and therapeutic implications

This article is made available through the Brunel Open Access Publishing Fund. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.CLU (clusterin) is a tumor suppressor gene that we have previously shown to be negatively modulated by the MYCN proto-oncogene, but the mechanism of repression was unclear. Here, we show that MYCN inhibits the expression of CLU by direct interaction with the non-canonical E box sequence CACGCG in the 5′-flanking region. Binding of MYCN to the CLU gene induces bivalent epigenetic marks and recruitment of repressive proteins such as histone deacetylases and Polycomb members. MYCN physically binds in vitro and in vivo to EZH2, a component of the Polycomb repressive complex 2, required to repress CLU. Notably, EZH2 interacts with the Myc box domain 3, a segment of MYC known to be essential for its transforming effects. The expression of CLU can be restored in MYCN-amplified cells by epigenetic drugs with therapeutic results. Importantly, the anticancer effects of the drugs are ablated if CLU expression is blunted by RNA interference. Our study implies that MYC tumorigenesis can be effectively antagonized by epigenetic drugs that interfere with the recruitment of chromatin modifiers at repressive E boxes of tumor suppressor genes such as CLU.SPARKS, The Neuroblastoma Society, a Wellcome Trust grant (to A. S.), and the Italian Association for Cancer Research

\u27Dermo\u27 fleece rot and body strike on sheep

Blowfly strike is one of the major problems confronting the sheep industry in Austrsalia, with an estimated totsalcost of control eceeding $100 million each year. Sheep are susceptible to five types of strike: body, breach, poll, pizzle and wound. Body strike, is of greater economic importance because its sporadic occurence from year to year makes it difficult to predict and cintrol. Outbreaks of body strike after rain can affect large numbers of sheep and inflict severe production losses. In eastern Australia fleece rot is generally considered to be the main predisposing factor to blowfly strike, but in Western Australia evidence suggests that dermatophilosis - or \u27dermo\u27 - is the more important factor

Pastures from Space - Application of Satellite-Derived Pasture Predictions Improve the Profitability of Australian Sheep Producers

Pastures from Space, a collaborative program between CSIRO Livestock Industries and the Western Australian state Departments of Agriculture and Land Information, has developed the capacity to measure both the biomass and growth rate of annual pasture in the winter rainfall regions of southern Australia using satellite images (Edirisinghe et al., 2002). Producer groups were set up to pilot test the delivery of satellitederived pasture growth rate (PGR, kg dry matter/hectare.day) and biomass (feed on offer or FOO, kg dry matter/hectare) predictions for paddocks on individual farms in Western Australia. This paper reports on the value to Australian sheep producers of satellite-derived PGR information on pastures

Free and Dissolved Gases in Castrocaro Spa Waters (Italy)

Free and dissolved gases in cold water samples from the Castrocaro spa, Northern Italy, were analyzed for their chemical composition. These gases were interpreted as the result of the binary mixing between a N2- and a CH4-rich component. CO2 is generally a minor constituent. N2/Ar ratios below the air typical value suggest that air saturated water (ASW) is the most likely source of atmospheric-derived components. This atmospheric end-member is predominant in low-salinity waters. Conversely, CH4-enriched gases are mainly associated with brackish to saline waters. The occurrence of minor amounts of light hydrocarbons (C2-C3) indicates a predominant biogenic origin of CH4. The He isotopic composition of the CH4-richest sample (3He/4He = 0.22 Ra) is in the range of values measured for cold seeps and mud volcanoes along the Northern Apennines foothills, and indicates a predominant crustal origin of this gas

Earthquake-Related Signals in Central Italy Detected by Hydrogeochemical and Satellite Techniques

Central Apennines are one of the highest seismic risk regions in Italy. A number of energetic events ((Formula presented.) > 5) struck the region during the period 2004–2017, killing several hundreds of people (e.g., 294 casualties associated with the August 24th, 2016, (Formula presented.) 6.0 event of Amatrice). These earthquakes impacted piezometric levels, springs discharges, and groundwater chemistry across a large area, even at distances of dozens of kilometers from the epicenters. Here we present a multidisciplinary dataset based on hydrogeochemical and satellite observations associated with the seismic events that occurred in Central Italy during the period 2004–2017, which combines information derived from the application of groundwater monitoring and satellite techniques. Groundwater monitoring techniques allowed for the detection of hydrogeochemical anomalies in spring and well waters (14 water sampling points in total, with 22 variations larger than (Formula presented.)), while satellite techniques were applied to detect time-space variations in ground thermal emissions. We detected two significant, almost synchronous, anomalies in 2009 and 2016–2017 with both techniques, and we tentatively correlated them to crustal deformation processes. Part of the observed signals were detected before mainshocks, and they appear to be related to aseismic slip or to seismic slip eventually induced by minor fluctuations in seismicity. We argue that the combination of two factors, i.e., the shallow depth of local earthquakes and the concurrent deepening of groundwater circulation paths to several km depth, allow for the recording of variations in the stress field by geofluids released at the surface

European Non-native Species in Aquaculture Risk Analysis Scheme - a summary of assessment protocols and decision support tools for use of alien species in aquaculture

The European Non-native Species in Aquaculture Risk Analysis Scheme (ENSARS) was developed in response to European 'Council Regulation No. 708/2007 of 11 June 2007 concerning use of alien and locally absent species in aquaculture' to provide protocols for identifying and evaluating the potential risks of using non-native species in aquaculture. ENSARS is modular in structure and adapted from non-native species risk assessment schemes developed by the European and Mediterranean Plant Protection Organisation and for the UK. Seven of the eight ENSARS modules contain protocols for evaluating the risks of escape, introduction to and establishment in open waters, of any non-native aquatic organism being used (or associated with those used) in aquaculture, that is, transport pathways, rearing facilities, infectious agents, and the potential organism, ecosystem and socio-economic impacts. A concluding module is designed to summarise the risks and consider management options. During the assessments, each question requires the assessor to provide a response and confidence ranking for that response based on expert opinion. Each module can also be used individually, and each requires a specific form of expertise. Therefore, a multidisciplinary assessment team is recommended for its completion