Deamidation at Asparagine and Glutamine As a Major Modification upon Deterioration/Aging of Proteinaceous Binders in MuralPaintings

Proteomic strategies are herein proved to be a complementary approach to the well established amino acid composition analysis for the characterization of the aging and deterioration phenomena occurring to proteinaceous materials in works-of-art. Amino acid analyses on several samples demonstrated that proteins in the frescoes from the Camposanto Monumentale in Pisa are deteriorated as revealed by the decrease in Met, Lys, and Tyr content and by the presence in all the samples of amino malonic acid as a result of Ser, Phe, and Cys oxidation. Proteomic analysis identified deamidation at Asn and Gln as a further major event occurred. This work paves the way to the exploitation of proteomic strategies for the investigation of the molecular effects of aging and deterioration in historical objects. Results show that proteomic searches for deamidation by liquid chromatography-tandem mass spectrometry (LC-MS/MS) could constitute a routine analysis for paintings or any artistic and historic objects where proteins are present. Peptides that can be used as molecular markers when casein is present were identified

Rate-Control or Rhythm-Contol: Where do we stand?

Atrial fibrillation is the most common sustained rhythm disturbance and its prevalence is increasing worldwide due to the progressive aging of the population. Current guidelines clearly depict the gold standard management of acute symptomatic atrial fibrillation but the best-long term approach for first or recurrent atrial fibrillation is still debated with regard to quality of life, risk of new hospitalizations, and possible disabling complications, such as thromboembolic stroke, major bleeds and death. Some authors propose that regaining sinus rhythm in all cases, thus re-establishing a physiologic cardiac function not requiring a prolonged antithrombotic therapy, avoids the threat of intracranial or extracranial haemorrhages due to Vitamin K antagonists or aspirin. On the contrary, advocates of a rate control approach with an accurate antithrombotic prophylaxis propose that such a strategy may avoid the risk of cardiovascular and non cardiovascular side effects related to antiarrhythmic drugs. This review aims to explore the state of our knowledge in order to summarize evidences and issues that need to be furthermore clarified

Search for α\alpha decay of 151^{151}Eu to the first excited level of 147^{147}Pm using underground γ\gamma-ray spectrometry

The alpha decay of $^{151}$Eu to the first excited level of $^{147}$Pm ($J^\pi = 5/2^+$, $E_{exc}=91.1$ keV) was searched for at the HADES underground laboratory ($\approx 500$ m w.e.). A sample of high purity europium oxide with mass of 303 g and a natural isotopic composition has been measured over 2232.8 h with a high energy resolution ultra-low background n-type semi-planar HPGe detector (40 cm$^3$) with sub-micron deadlayer. The new improved half-life limit has been set as $T_{1/2} \geq 3.7\times 10^{18}$ yr at 68% C.L. Possibilities to improve the sensitivity of the experiment, which is already near the theoretical predictions, are discussed. New half-life limit for $\alpha$ decay of $^{153}$Eu is also set as $T_{1/2} \geq 5.5\times 10^{17}$ yr.Comment: 11 pages, 5 figures, 2 tables, 18 reference

Flow rule, self-channelization and levees in unconfined granular flows

Unconfined granular flows along an inclined plane are investigated experimentally. During a long transient, the flow gets confined by quasistatic banks but still spreads laterally towards a well-defined asymptotic state following a nontrivial process. Far enough from the banks a scaling for the depth averaged velocity is obtained, which extends the one obtained for homogeneous steady flows. Close to jamming it exhibits a crossover towards a nonlocal rheology. We show that the levees, commonly observed along the sides of the deposit upon interruption of the flow, disappear for long flow durations. We demonstrate that the morphology of the deposit builds up during the flow, in the form of an underlying static layer, which can be deduced from surface velocity profiles, by imposing the same flow rule everywhere in the flow.Comment: 4 pages, 5 figure

Erosion waves: transverse instabilities and fingering

Two laboratory scale experiments of dry and under-water avalanches of non-cohesive granular materials are investigated. We trigger solitary waves and study the conditions under which the front is transversally stable. We show the existence of a linear instability followed by a coarsening dynamics and finally the onset of a fingering pattern. Due to the different operating conditions, both experiments strongly differ by the spatial and time scales involved. Nevertheless, the quantitative agreement between the stability diagram, the wavelengths selected and the avalanche morphology reveals a common scenario for an erosion/deposition process.Comment: 4 pages, 6 figures, submitted to PR

Elastic deformation due to tangential capillary forces \ud

A sessile liquid drop can deform the substrate on which it rests if the solid is sufficiently “soft.” In this paper we compute the detailed spatial structure of the capillary forces exerted by the drop on the solid substrate using a model based on Density Functional Theory. We show that, in addition to the normal forces, the drop exerts a previously unaccounted tangential force. The resultant effect on the solid is a pulling force near the contact line directed towards the interior of the drop, i.e., not along the interface. The resulting elastic deformations of the solid are worked out and illustrate the importance of the tangential force

Contact angles on a soft solid: from Young's law to Neumann's law

The contact angle that a liquid drop makes on a soft substrate does not obey the classical Young's relation, since the solid is deformed elastically by the action of the capillary forces. The finite elasticity of the solid also renders the contact angles different from that predicted by Neumann's law, which applies when the drop is floating on another liquid. Here we derive an elasto-capillary model for contact angles on a soft solid, by coupling a mean-field model for the molecular interactions to elasticity. We demonstrate that the limit of vanishing elastic modulus yields Neumann's law or a slight variation thereof, depending on the force transmission in the solid surface layer. The change in contact angle from the rigid limit (Young) to the soft limit (Neumann) appears when the length scale defined by the ratio of surface tension to elastic modulus $\gamma/E$ reaches a few molecular sizes

Szeg\"o kernel asymptotics and Morse inequalities on CR manifolds

We consider an abstract compact orientable Cauchy-Riemann manifold endowed with a Cauchy-Riemann complex line bundle. We assume that the manifold satisfies condition Y(q) everywhere. In this paper we obtain a scaling upper-bound for the Szeg\"o kernel on (0, q)-forms with values in the high tensor powers of the line bundle. This gives after integration weak Morse inequalities, analogues of the holomorphic Morse inequalities of Demailly. By a refined spectral analysis we obtain also strong Morse inequalities which we apply to the embedding of some convex-concave manifolds.Comment: 40 pages, the constants in Theorems 1.1-1.8 have been modified by a multiplicative constant 1/2 ; v.2 is a final updat

A mutant of phosphomannomutase1 retains full enzymatic activity, but is not activated by IMP : possible implications for the disease PMM2-CDG

The most frequent disorder of glycosylation, PMM2-CDG, is caused by a deficiency of phosphomannomutase activity. In humans two paralogous enzymes exist, both of them require mannose 1,6-bis-phosphate or glucose 1,6-bis-phosphate as activators, but only phospho-mannomutase1 hydrolyzes bis-phosphate hexoses. Mutations in the gene encoding phosphomannomutase2 are responsible for PMM2-CDG. Although not directly causative of the disease, the role of the paralogous enzyme in the disease should be clarified. Phosphomannomutase1 could have a beneficial effect, contributing to mannose 6-phosphate isomerization, or a detrimental effect, hydrolyzing the bis-phosphate hexose activator. A pivotal role in regulating mannose-1phosphate production and ultimately protein glycosylation might be played by inosine monophosphate that enhances the phosphatase activity of phosphomannomutase1. In this paper we analyzed human phosphomannomutases by conventional enzymatic assays as well as by novel techniques such as 31P-NMR and thermal shift assay. We characterized a triple mutant of phospomannomutase1 that retains mutase and phosphatase activity, but is unable to bind inosine monophosphate

The Kinetic Effect of Some Wine Components on the Enzymatic Hydrolysis of β-glucan

Enzymatic preparations containing β-glucanases are utilised extensively in winemaking to facilitate the filtration ofmusts and wines coming from grapes affected by Botrytis cinerea, and to induce the release of mannoproteins andoligosaccharides from the cell walls of yeasts. The aim of the present work was to investigate the possible inhibitoryeffect of some wine components, and in particular of ethanol, on β-glucanase activities. For this purpose, the kineticactivity of a commercial enzymatic preparation containing β-glucanases was tested utilising both model solutions(buffer solution having a pH value similar to a must/wine with or without 13% v/v of ethanol added) and a redwine. When ethanol was added to the model solution, both the kinetic constant k and glucose production suffered adecrease of about 50% compared to the values detected in the absence of ethanol. A further loss of activity (about87%) was found using red wine as reaction medium, suggesting, as already reported in the literature, that phenolscould add their inhibitory effect to that of ethanol. The results obtained provide useful suggestions that can beadopted during winemaking. To promote the hydrolysis of the possible excess of β-glucan coming from grapesaffected by Botritis cinerea, it would be more convenient to add β-glucanases before a significant amount of ethanolaccumulates in the must/wine, while it is not possible to avoid the negative impact of ethanol or other possibleinhibitors (phenols) to promote yeast degradation in wine left on lees