Aspectos epidemiológicos da hepatite B a partir do Sistema de Informação de Agravos de Notificação (SINAM) do Estado da Bahia

Introdução: Na estrutura da Organização Mundial de Saúde (OMS) existem 50 indicadores de saúde que avaliam os seus 192 países-membro. Entre os parâmetros de análise, as doenças de notificação compulsória caracterizam a situação de saúde de cada um desses países. Sabe-se que cerca de 35 milhões de pessoas morreram no ano de 2005 por doenças crônicas. No Brasil, estimava-se 2 milhões de portadores crônicos do vírus da hepatite B (VHB) em 2008, apesar das inúmeras estratégias de incentivo a imunoprofilaxia. Objetivo: Analisar dados de hepatite B na Bahia, registrados no  Sistema de Informação de Agravos de Notificação (SINAM) /DATASUS/MS. Material e Métodos: Busca e análise de dados registrados no  Sistema de Informação de Agravos de Notificação (SINAM) do Ministério da Saúde, no período de 2007 a 2012. Os parâmetros da pesquisa no SINAM foram: número de casos, gênero, idade; a positividade do AgHBs; além de Salvador, os vários municípios do estado com população acima de 100 mil habitantes. Resultados: Na Bahia, dos 1070 casos de hepatite viral notificados na cidade de Salvador, nos últimos cinco anos, 201 pacientes apresentavam confirmação diagnóstica de AgHBs positivo. Houve associação significativa no gênero masculino e a faixa etária de 20 a 39 anos. Conclusão: A maior prevalência de hepatite B notificada foi de homens adultos jovens e maduros, na cidade de Salvador, Não  houve diminuição de casos com o tempo e não existiu proporcionalidade de número de casos com cidade mais populosa.   Abstract Introduction: In the World Health Organization (WHO) framework there are fifty health indicators which evaluate their 192 member countries. Among the analysis parameters, diseases which require notification characterize the health situation in these countries.  It is known that about 35 million people died from chronic diseases in 2005. In Brazil, it was estimated that there were 2 million chronic carriers of hepatitis B virus (HBV) in 2008, despite the countless strategies to encourage immunoprophylaxis. Objective: To analyze hepatitis B data in Bahia registered in Brazilian Case Registry Database (SINAM) /DATASUS/MS. Material and Methods: Search and analysis of data recorded in the Brazilian Case Registry Database (SINAM) of the Ministry of Health from 2007 to 2012. The research parameters in SINAM were: number of cases, gender, age, the HBsAg positivity not only in Salvador, but in various cities with population over 100,000 in the state. Results: In Bahia, in the last five years, 1070 cases of viral hepatitis reported in the city of Salvador, 201 patients were diagnosed with HBsAg positive. There was a significant association in males and aged 40 to 59 years. Conclusion: The highest prevalence of hepatitis B notified was among men and mature young adults in the city of Salvador in the period. There was no reduction in the number of cases throughout time as well as there was no proportionality with the number of cases in more populous citie

Aspectos epidemiológicos da hepatite B a partir do Sistema de Informação de Agravos de Notificação (SINAM) do Estado da Bahia

Introdução: Na estrutura da Organização Mundial de Saúde (OMS) existem 50 indicadores de saúde que avaliam os seus 192 países-membro. Entre os parâmetros de análise, as doenças de notificação compulsória caracterizam a situação de saúde de cada um desses países. Sabe-se que cerca de 35 milhões de pessoas morreram no ano de 2005 por doenças crônicas. No Brasil, estimava-se 2 milhões de portadores crônicos do vírus da hepatite B (VHB) em 2008, apesar das inúmeras estratégias de incentivo a imunoprofilaxia. Objetivo: Analisar dados de hepatite B na Bahia, registrados no  Sistema de Informação de Agravos de Notificação (SINAM) /DATASUS/MS. Material e Métodos: Busca e análise de dados registrados no  Sistema de Informação de Agravos de Notificação (SINAM) do Ministério da Saúde, no período de 2007 a 2012. Os parâmetros da pesquisa no SINAM foram: número de casos, gênero, idade; a positividade do AgHBs; além de Salvador, os vários municípios do estado com população acima de 100 mil habitantes. Resultados: Na Bahia, dos 1070 casos de hepatite viral notificados na cidade de Salvador, nos últimos cinco anos, 201 pacientes apresentavam confirmação diagnóstica de AgHBs positivo. Houve associação significativa no gênero masculino e a faixa etária de 20 a 39 anos. Conclusão: A maior prevalência de hepatite B notificada foi de homens adultos jovens e maduros, na cidade de Salvador, Não  houve diminuição de casos com o tempo e não existiu proporcionalidade de número de casos com cidade mais populosa.   Abstract Introduction: In the World Health Organization (WHO) framework there are fifty health indicators which evaluate their 192 member countries. Among the analysis parameters, diseases which require notification characterize the health situation in these countries.  It is known that about 35 million people died from chronic diseases in 2005. In Brazil, it was estimated that there were 2 million chronic carriers of hepatitis B virus (HBV) in 2008, despite the countless strategies to encourage immunoprophylaxis. Objective: To analyze hepatitis B data in Bahia registered in Brazilian Case Registry Database (SINAM) /DATASUS/MS. Material and Methods: Search and analysis of data recorded in the Brazilian Case Registry Database (SINAM) of the Ministry of Health from 2007 to 2012. The research parameters in SINAM were: number of cases, gender, age, the HBsAg positivity not only in Salvador, but in various cities with population over 100,000 in the state. Results: In Bahia, in the last five years, 1070 cases of viral hepatitis reported in the city of Salvador, 201 patients were diagnosed with HBsAg positive. There was a significant association in males and aged 40 to 59 years. Conclusion: The highest prevalence of hepatitis B notified was among men and mature young adults in the city of Salvador in the period. There was no reduction in the number of cases throughout time as well as there was no proportionality with the number of cases in more populous citie

Quantification and Comprehensive Analysis of Mesenchymal Stromal Cells in Bone Marrow Samples from Sickle Cell Disease Patients with Osteonecrosis

The potential use of bone marrow mesenchymal stromal cells (BM-MSCs) for the treatment of osteonecrosis in sickle cell disease (SCD) patients is increasing. However, convenient BM-MSC quantification and functional property assays are critical factors for cell-based therapies yet to be optimized. This study was designed to quantify the MSC population in bone marrow (BM) samples from SCD patients with osteonecrosis (SCD group) and patients with osteoarticular complications not related to SCD (NS group), using flow cytometry for CD271+CD45-/low cell phenotype and CFU-F assay. We also compared expanded BM-MSC osteogenic differentiation, migration, and cytokine secretion potential between these groups. The mean total cell number, CFU-F count, and CD271+CD45-/low cells in BM mononuclear concentrate were significantly higher in SCD than in NS patients. A significant correlation between CD271+CD45-/low cell number and CFU-F counts was found in SCD (r=0.7483; p=0.0070) and NS (r=0.7167; p=0.0370) BM concentrates. An age-related quantitative reduction of CFU-F counts and CD271+CD45-/low cell number was noted. Furthermore, no significant differences in the morphology, replicative capacity, expression of surface markers, multidifferentiation potential, and secretion of cytokines were found in expanded BM-MSCs from SCD and NS groups after in vitro culturing. Collectively, this work provides important data for the suitable measurement and expansion of BM-MSC in support to advanced cell-based therapies for SCD patients with osteonecrosis

HCV/HTLV coinfection: Does HTLV-1 interfere in the natural history of HCV-related diseases?

Edgard Marcelino de Carvalho Filho. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. "Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento".Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-04-07T12:51:56Z No. of bitstreams: 1 Silva MC HCV HTLV coinfection....pdf: 116203 bytes, checksum: 24e464dc01689486d0e327d8242ac287 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-04-07T13:05:39Z (GMT) No. of bitstreams: 1 Silva MC HCV HTLV coinfection....pdf: 116203 bytes, checksum: 24e464dc01689486d0e327d8242ac287 (MD5)Made available in DSpace on 2017-04-07T13:05:39Z (GMT). No. of bitstreams: 1 Silva MC HCV HTLV coinfection....pdf: 116203 bytes, checksum: 24e464dc01689486d0e327d8242ac287 (MD5) Previous issue date: 2016CNPq (Universal); Grant sponsor: LIA ProjectFederal University of Bahia. Department of Hepatology. Salvador, BA, BrazilFederal University of Bahia. Department of Hepatology. Salvador, BA, BrazilFederal University of Bahia. Department of Hepatology. Salvador, BA, BrazilFederal University of Bahia. Clinical and Toxicological Analysis. Salvador, BA, BrazilFederal University of Bahia. Immunology Laboratory of the Health Science Institute. Salvador, BA, BrazilFederal University of Bahia. Immunology Service of the University Hospital Professor Edgar Santos. Salvador, BA, BrazilFederal University of Bahia. Hepatology Center of the University Hospital Professor Edgar Santos. Salvador, BA, BrazilFederal University of Bahia. Hepatology Center of the University Hospital Professor Edgar Santos. Salvador, BA, BrazilHepatitis C virus (HCV) and human T-lymphotropic virus type 1 (HTLV-1) coinfection occurs in many regions. However, few studies have focused on the natural history of HCV-induced liver disease in coinfected patients. To describe the clinical, epidemiological, and histopathological aspects of HTLV-1/HCV coinfection in Brazil. A cross-sectional study with 23 patients coinfected with HCV/HTLV. The control groups consisted of 21 patients monoinfected with HCV and 20 patients monoinfected with HTLV-1. The cytokine profiles (Th1 and Th2 cell responses), clinical, laboratory features, and histopathological aspects were examined. The control group for cytokine analysis validation consisted of patients monoinfected with HTLV, and a fourth group consisted of healthy blood donors. No anthropometric differences present between the three infected groups. We observed higher serum concentrations of IFN-γ in patients coinfected with HCV/HTLV-1 than those in HCV monoinfected patients. The HCV/HTLV-1 coinfected group also exhibited a higher degree of liver steatosis than the HCV monoinfected patients. Results suggest that HCV/HTLV-1 coinfection may result in a different pattern of HCV infection due to the immunologic disorders likely associated with HTLV-1, but there is no clear evidence of the HTLV role in the natural history of HCV infection

Ancestry informative markers and complete blood count parameters in Brazilian blood donors Marcadores informativos de ancestralidade e parâmetros no hemograma de doadores de sangue brasileiros

A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs) were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observed between gender and neutrophils, monocytes, eosinophils, erythrocytes, hemoglobin, hematocrit, MCV, MCHC and platelet counts. No significant differences were found for age, alcohol consumption, educational status, ethnicity, smoking in respect to the complete blood count values. In general, men had higher red blood cell values, while women had higher values for white blood cells and platelets. The study of the population was highly heterogeneous with mean proportions (± SE) of African, European and Amerindian ancestry being 49.0 ± 3.0%, 44.0 ± 9.0% and 7.0 ± 9.0%, respectively. Amerindian ancestry showed limited contribution to the makeup of the population, but estimated ancestral proportions were statistically significant (r = 0.9838; P<0.001). These hematologic values are similar to Afro-Americans, another admixed population.<br>O hemograma é muito útil no diagnóstico quando o intervalo de referência é adequadamente estabelecido para população. Com o objetivo de verificar os valores hematológicos em população heterogênea foi analisado o hemograma e frequências alélica de marcadores informativos de ancestralidade de brasileiros. Foi observada associação positiva entre sexo e os valores de neutrófilos, monócitos, eosinófilos, eritrócitos, hemoglobina, hematócrito, MCV, MCHC e plaquetas (IC 95%; P<0,05). E não houve diferenças entre idade, consumo de álcool, nível educacional, etnia, tabagismo e os valores do hemograma (IC 95%; P>0,05). Os homens apresentaram valores maiores no eritrograma, enquanto no leucograma e plaquetograma foram as mulheres. Foi observado também que a população é altamente heterogênea e as médias proporcionais (±DP) de ancestralidade Africana, Europeia e Ameríndia estimada foram: 49,0 ± 3,0 %, 44,0 ± 9,0% e 7,0 ± 9,0%, respectivamente. A contribuição ancestral ameríndia se demonstrou pequena, mas a estimativa de proporções ancestrais foi estatisticamente significante (r = 0,9838; P<0,001). Os valores hematológicos aqui descritos são parecidos com os descritos em negros americanos, outra população heterogênea

Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG+874 A>T polymorphism

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-13T14:13:22Z No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-13T14:23:58Z (GMT) No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5)Made available in DSpace on 2018-08-13T14:23:58Z (GMT). No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5) Previous issue date: 2018Conselho Nacional de Desenvolvimento Científico e Tecnológico (INCT-DT/MCT/CNPq 573839/2008-5) and Fundação de Amparo à Pesquisa do Estado da Bahia (PET035/2013).Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Fundação José Silveira. Instituto Brasileiro para a Investigação da Tuberculose. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilLatent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG+874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG+874 A/T polymorphism on interferon-gamma test performance. Methods: Patients diagnosed with pulmonary tuberculosis (75), volunteers with positivetuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no his-tory of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype andthe IFN- levels in whole blood cultures performed using an interferon-gamma commercialkit (QuantiFERON-TB®Gold In-Tube).Results: IFN- production was not influenced by the IFNG + 874 genotype, regardless of anti-gen or mitogen-based stimulation, which suggests that other genes may influence IFN- production in response to mycobacteria. The IFNG + 874 polymorphism was found to exertno influence over QFT-IT test sensitivity in our study

Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A>T polymorphism

ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype and the IFN-γ levels in whole blood cultures performed using an interferon-gamma commercial kit (QuantiFERON-TB® Gold In-Tube). Results IFN-γ production was not influenced by the IFNG + 874 genotype, regardless of antigen or mitogen-based stimulation, which suggests that other genes may influence IFN-γ production in response to mycobacteria. The IFNG + 874 polymorphism was found to exert no influence over QFT-IT test sensitivity in our study. Conclusions The IFNG + 874 polymorphism was not shown to influence QuantiFERON-TB® Gold In-Tube test performance in an admixed population from northeastern Brazil

Ancestry informative markers and complete blood count parameters in Brazilian blood donors

A complete blood count is very useful in clinical diagnoses when reference ranges are well established for the population. Complete blood counts and allele frequencies of Ancestry Informative Markers (AIMs) were analyzed in Brazilians with the aim of characterizing the hematological values of an admixed population. Positive associations were observed between gender and neutrophils, monocytes, eosinophils, erythrocytes, hemoglobin, hematocrit, MCV, MCHC and platelet counts. No significant differences were found for age, alcohol consumption, educational status, ethnicity, smoking in respect to the complete blood count values. In general, men had higher red blood cell values, while women had higher values for white blood cells and platelets. The study of the population was highly heterogeneous with mean proportions (± SE) of African, European and Amerindian ancestry being 49.0 ± 3.0%, 44.0 ± 9.0% and 7.0 ± 9.0%, respectively. Amerindian ancestry showed limited contribution to the makeup of the population, but estimated ancestral proportions were statistically significant (r = 0.9838; P<0.001). These hematologic values are similar to Afro-Americans, another admixed population