1,668 research outputs found
Stratospheric aerosol extinction profile retrievals from SCIAMACHY limb-scatter observations
This dissertation presents a method for retrieving stratospheric aerosol extinction profiles from a global satellite data set. Ten years of limb radiance measurements with the instrument SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) onboard the European environmental research satellite Envisat provides the unique opportunity to derive a stratospheric aerosol extinction data set over a long time period (2002 2012) with a good global coverage on a daily basis. Stratospheric sulfate aerosols have a significant impact on climate but the determination is still subject to large uncertainties. To improve our understanding of climate relevant processes an accurate determination of stratospheric aerosol properties is crucial. Deriving stratospheric aerosol extinction from limb radiance spectra requires complicated radiative transfer calculations. An algorithm based on a color-index approach combining normalized limb radiance spectra at 470 and 750 nm is applied to retrieve aerosol extinction profiles between 12 and 35 km altitude. A Mie phase function for typical background aerosols is implemented. The resulting SCIAMACHY stratospheric aerosol data set can serve as a foundation for climatological interpretation with respect to natural variability and anthropogenic impact
Transcriptional Regulation of the Nickel and Iron Metabolism in Helicobacter pylori
Up to 50 % of the world's population is infected with Helicobacter pylori. Colonization of
the mucus layer of the human stomach by H. pylori, is lifelong unless treated with antibiotics
(26). H. pylori, which is a neutralophilic bacterium, survives in the mucus layer of the human
stomach with the help of the enzyme urease. Urease is an enzyme that converts urea into
ammonium and carbon dioxide, thereby keeping the intracellular and periplasmic pH at
neutral. It is estimated that up to 10% of the whole cell protein consists of this nickelcofactored
enzyme (19). The nickel necessary to activate the urease is thought to come from
foodsources, such as nuts, tea and cereals, which are rich in nickel (1).
Metal ions like nickel or iron can be dangerous for bacteria, as they can react with oxygen
in order to create reactive oxygen species that in turn can destroy macromolecules like
nucleic acids, proteins and cell wall components (27). Therefore, the bacterial metal
metabolism has to be tightly regulated. In H. pylori, regulatory proteins are scarce. Only two
metal-regulatory proteins are known, the ferric uptake regulator Fur (4), and the nickel
responsive regulator NikR (31). Fur is a regulatory protein that can sense and bind
intracellular ferrous ions, and subsequently displays iron-dependent binding to conserved
promoter sequences (Fur boxes) of its target genes (17). The classical regulation is repression
of iron uptake genes in iron-replete conditions (15, 17, 33). Unlike all other Fur homologs
known so far, H. pylori Fur can also bind to Fur-boxes in an iron free form (apo-Fur), as was
shown for pfr (16) and sodB (Chapter 3).
The second metal-dependent regulatory protein is NikR, the nickel responsive regulator,
which belongs to the family of Ribbon-Helix-Helix regulatory proteins (9). NikR is directly
involved in the regulation of acid resistance via urease and nickel-uptake (Chapter 4), and
was previously demonstrated to mediate regulation of the ferric uptake regulator Fur (7, 12,
29).
The aim of this thesis was to gain further insight into the transcriptional regulation the
ferric uptake regulator Fur and the nickel responsive regulator NikR
miR-200c sensitizes breast cancer cells to doxorubicin treatment by decreasing TrkB and Bmi1 expression.
Acquired resistance to classical chemotherapeutics is a major obstacle in cancer treatment. Doxorubicin is frequently used in breast cancer therapy either as single-agent or in combination with other drugs like docetaxel and cyclophosphamide. All these chemotherapies have in common that they are administered sequentially and often result in chemoresistance. Here, we mimicked this pulse therapy of breast cancer patients in an in vitro cell culture model, where the epithelial breast cancer cell line BT474 was sequentially treated with doxorubicin for several treatment cycles. In consequence, we obtained chemoresistant cells displaying a mesenchymal-like phenotype with decreased levels of miR-200c. To investigate the involvement of miR-200c in resistance formation, we inhibited and overexpressed miR-200c in different cell lines. Thereby, the cells were rendered more resistant or susceptible to doxorubicin treatment. Moreover, the receptor tyrosine kinase TrkB and the transcriptional repressor Bmi1 were identified as miR-200c targets mediating the drug resistance. Hence, we provide a mechanism of acquired resistance to doxorubicin that is caused by the loss of miR-200c. Along with this, our study demonstrates the complex network of microRNA mediated chemoresistance highlighting the challenges in cancer therapy and the importance of novel microRNA-modulating anticancer agents
Soft Gamma Rays from Heavy WIMPs
We propose an explanation of the galactic center gamma ray excess by
supersymmetric WIMPs as heavy as 500 GeV. The lightest neutralino annihilates
into vector-like leptons or quarks which cascade decay through intermediate
Higgs bosons. Due to the long decay chains, the gamma ray spectrum is much
softer than naively expected and peaks at GeV energies. The model predicts
correlated diboson and dijet signatures to be tested at the LHC.Comment: 8 pages, 8 figures; v2: focus on gamma ray excess, matches published
versio
E- and P-Selectin : Differences, Similarities and Implications for the Design of P-Selectin Antagonists
Adhesion Properties of Hydrogen on Sb(111) Probed by Helium Atom Scattering
We have carried out a series of helium atom scattering measurements in order
to characterise the adsorption properties of hydrogen on antimony(111).
Molecular hydrogen does not adsorb at temperatures above 110 K in contrast to
pre-dissociated atomic hydrogen. Depending on the substrate temperature, two
different adlayer phases of atomic hydrogen on Sb(111) occur. At low substrate
temperatures (K), the deposited hydrogen layer does not show any ordering
while we observe a perfectly ordered H/Sb(111) structure for
deposition at room temperature. Furthermore, the amorphous hydrogen layer
deposited at low temperature forms an ordered overlayer upon heating the
crystal to room temperature. Hydrogen starts to desorb at K which
corresponds to a desorption energy of eV. Using
measurements of the helium reflectivity during hydrogen exposure at different
surface temperatures, we conclude that the initial sticking coefficient of
atomic hydrogen on Sb(111) decreases with increasing surface temperature.
Furthermore, the scattering cross section for the diffuse scattering of helium
from hydrogen on Sb(111) is determined as \Sigma = (12 \pm 1)~\mbox{\AA}^{2}.Comment: 7 pages, 5 figure
Photoinduced molecule formation of spatially separated atoms on helium nanodroplets
Besides the use as cold matrix for spectroscopic studies, superfluid helium
droplets have served as a cold environment for the synthesis of molecules and
clusters. Since vibrational frequencies of molecules in helium droplets exhibit
almost no shift compared to the free molecule values, one could assume the
solvated particles move frictionless and undergo a reaction as soon as their
paths cross. There have been a few unexplained observations that seemed to
indicate cases of two species on one droplet not forming bonds but remaining
isolated. In this work, we performed a systematic study of helium droplets
doped with one rubidium and one strontium atom showing that besides a reaction
to RbSr, there is a probability of finding separated Rb and Sr atoms on one
droplet that only react after electronic excitation. Our results further
indicate that ground-state Sr atoms can reside at the surface as well as inside
the droplet
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