Bendings of radio jets in BL Lacertae objects I: EVN and MERLIN observations

Several blazars, and BL Lac objects in particular, show a misalignment between the jet orientation on parsec and kiloparsec scales. Some authors (i.e. Conway & Murphy, 1993) have attempted to explain this behaviour invoking helical jets for misalignment angles around 90\degr, showing how in this case there are interesting implications for the understanding of the medium into which the jet is expanding. By comparing sensitive VLA observations (Cassaro et al., 1999) with images available in the literature for the BL Lac objects from the 1-Jy Sample (Stickel et al., 1991), it is clear that there is a wide range of misalignments between the initial jet direction and the kpc-scale jet, when detected. We have carried out VLBI observations of these BL Lac objects, in order to investigate the spatial evolution of the radio jets from few tens to hundreds of mas, and to search for helical jets in this class of sources. We present here the first dataset obtained from EVN+MERLIN observations at 5 GHz for seven objects. From these observations we never have a clear detection of helical jets, we only have a possible signature of their presence in 2 objects. In only one of the sources with a misalignment angle around 90\degr the presence of helical jets can be ruled out. This implies that it is not possible to invoke helical jets to explain the morphology of all the sources showing a misalignment of about 90\degr between the parsec and the kiloparsec scale jets.Comment: 12 pages, 9 figures, latex, accepted by Astronomy & Astrophysic

Driving sandpiles to criticality and beyond

A popular theory of self-organized criticality relates driven dissipative systems to systems with conservation. This theory predicts that the stationary density of the abelian sandpile model equals the threshold density of the fixed-energy sandpile. We refute this prediction for a wide variety of underlying graphs, including the square grid. Driven dissipative sandpiles continue to evolve even after reaching criticality. This result casts doubt on the validity of using fixed-energy sandpiles to explore the critical behavior of the abelian sandpile model at stationarity.Comment: v4 adds referenc

Characterisation of Long Baseline Calibrators at 2.3 GHz

We present a detailed multi-epoch analysis of 31 potential southern hemisphere radio calibrators that were originally observed as part of a program to maintain the International Celestial Reference Frame (ICRF). At radio wavelengths, the primary calibrators are Active Galactic Nuclei (AGN), powerful radio emitters which exist at the centre of most galaxies. These are known to vary at all wavelengths at which they have been observed. By determining the amount of radio source structure and variability of these AGN, we determine their suitability as phase calibrators for long baseline radio interferometry at 2.3 GHz. For this purpose, we have used a set of complementary metrics to classify these 31 southern sources into five categories pertaining to their suitability as VLBI calibrators. We find that all of the sources in our sample would be good interferometric calibrators and almost ninety per cent would be very good calibrators.Comment: 9 pages, 7 Figures, accepted MNRA

Growth Rates and Explosions in Sandpiles

We study the abelian sandpile growth model, where n particles are added at the origin on a stable background configuration in Z^d. Any site with at least 2d particles then topples by sending one particle to each neighbor. We find that with constant background height h <= 2d-2, the diameter of the set of sites that topple has order n^{1/d}. This was previously known only for h<d. Our proof uses a strong form of the least action principle for sandpiles, and a novel method of background modification. We can extend this diameter bound to certain backgrounds in which an arbitrarily high fraction of sites have height 2d-1. On the other hand, we show that if the background height 2d-2 is augmented by 1 at an arbitrarily small fraction of sites chosen independently at random, then adding finitely many particles creates an explosion (a sandpile that never stabilizes).Comment: 19 pages, 4 figures, to appear in Journal of Statistical Physics. v2 corrects the proof of the outer bound of Theorem 4.1 of arXiv:0704.068

Proline biosynthesis regulates proline transport in Staphylococcus aureus.

Staphylococcus aureus is metabolically diverse with the ability to rapidly adapt to a vast array of nutrient sources. This allows the pathogen to colonize a variety of niches in the host. For instance, S. aureus is the leading cause of skin and soft tissue infections, a niche that has been shown to become glucose-depleted over the course of an infection. Previous studies have shown that in niches where glucose is deficient, S. aureus utilizes peptides and free amino acids as nutrient sources. Primarily, these amino acids include glutamate and amino acids that can serve as substrates for glutamate synthesis. While arginine and histidine serve as substrates in glutamate synthesis, proline is the primary source of glutamate. Indeed, S. aureus utilizes proline as a secondary carbon source only when glucose is absent, and it can be synthesized from arginine or acquired via proline transporters from its environment. Although S. aureus encodes two putative pathways for proline biosynthesis, it has been shown that pyrroline-5-carboxylate reductase (encoded by proC) is the sole proline biosynthetic pathway in S. aureus. Studies from our laboratory have revealed that despite encoding five putative proline transporters (B7H15_03660, opuC, opuD, proP, putP), only two of the transporters, PutP and B7H15_03660 are responsible for a majority of proline transport under the laboratory conditions tested. Surprisingly, when we introduced the proC mutation into the B7H15_03660 putP double mutant, we observed proline-dependent growth, even though the primary proline transporters and proline biosynthetic pathway were knocked-out. In contrast, a transporter null ΔproC strain was unable to grow. These data suggest that inhibiting proline biosynthesis alters proline transport, and therefore one or more of the additional transporters, OpuC, OpuD, and/or ProP, are activated under these conditions. After introducing opuC, opuD, and/or proP mutations into the Δ03660 ΔputP ΔproC strain, we found that both OpuC and ProP are important for proline transport. Additionally, we observed proline-dependent growth in a proline transporter null ΔproC strain when high amounts of exogenous proline are added to the media. This growth appears to be due to an acquired mutation and will be studied more in the future. Overall these studies have revealed that proline transport is tightly linked to proline biosynthesis.https://digitalcommons.unmc.edu/surp2021/1021/thumbnail.jp

A randomized crossover trial assessing patient preference for two different types of portable infusion-pump devices

Background: A variety of anticancer agents are better tolerated and more effective if given as continuous compared to bolus administration. Portable pump devices are needed to allow outpatient continuous infusion. Different types of portable pumps are available and we tested patient preference in a randomized crossover design. Patients and methods: Patients on continuous infusion fluorouracil were randomly assigned to start treatment with an elastomeric infusor (Baxter) or a mechanical, electronically controlled pump (CADD-1™, Pharmacia) and crossed over to the alternative model after three weeks. After exposure to both pump types patients were asked to indicate their preferred device. Results: After 10 patients the study was closed because all study participants preferred the elastomeric pump (P < 0.01). Reasons were pump weight (100%), smaller pump size (89%), interference with daily activities (89%), user friendliness (56%), impact on sleep (44%), and lack of technical problems (22%). Although the mechanical pump required more handling time for the first two refillings, the learning curve suggested about equal time requirement thereafter. Conclusion: In the interest of patient comfort, the disposable elastomeric infusor is an acceptable alternative to the more accurate electronically controlled pumps especially for drugs with a short half-life and a favorable toxicity profil

Contribution of the Staphylococcus aureus Atl AM and GL murein hydrolase activities in cell division, autolysis, and biofilm formation.

The most prominent murein hydrolase of Staphylococcus aureus, AtlA, is a bifunctional enzyme that undergoes proteolytic cleavage to yield two catalytically active proteins, an amidase (AM) and a glucosaminidase (GL). Although the bifunctional nature of AtlA has long been recognized, most studies have focused on the combined functions of this protein in cell wall metabolism and biofilm development. In this study, we generated mutant derivatives of the clinical S. aureus isolate, UAMS-1, in which one or both of the AM and GL domains of AtlA have been deleted. Examination of these strains revealed that each mutant exhibited growth rates comparable to the parental strain, but showed clumping phenotypes and lysis profiles that were distinct from the parental strain and each other, suggesting distinct roles in cell wall metabolism. Given the known function of autolysis in the release of genomic DNA for use as a biofilm matrix molecule, we also tested the mutants in biofilm assays and found both AM and GL necessary for biofilm development. Furthermore, the use of enzymatically inactive point mutations revealed that both AM and GL must be catalytically active for S. aureus to form a biofilm. The results of this study provide insight into the relative contributions of AM and GL in S. aureus and demonstrate the contribution of Atl-mediated lysis in biofilm development