Analisis Keberhasilan Penggunaan Perangkat Lunak Akuntansi Ditinjau Dari Persepsi Pemakai (Studi Implementasi Model Keberhasilan Sistem Informasi)

The purpose o f this study is to examine the information system (IS) success o f the accounting software based on the user perception. The model used to examine the IS success is the modified IS success model ofSeddon (1997). The model employed in this study is applied on data collected through 204 questionnaires distributed to the users o f accounting software who work at the variety o f companies in Indonesia. In examining the model, we employ the Structural Equation Model (SEM) with the use o f LISREL 8.72 sojH>are. The results o f the study show that system quality significantly affects the perceived usefulness and the user satisfaction. Furthermore, the results show that informc\tion quality significantly affects the perceived usefulness and user satisfaction. On the other hand, the study finds that user satisfaction does not affect the system use

Public space - the place, where we live?

This thesis focuses on the concept of public space as a construct which is formed at time and is a subject to the contemporary social conditions. The first part maps the historical evolution of this construct. The changes of content of terms that have been used at different times in the sense which today is understood as a public space are described. This part presents contemporary theoretical basis, their reflections in the society and also their reflection in the lived reality. It describes the key influences that shaped today's "modus vivendi" of public space. Apart from the ancient agora the work focuses on the Czech environment from the 19th century to the present. The second part, the research work describes how the current discourse of public space, represented by the Via Foundation (Nadace Via) and its program The place, where we live (Místo, kde žijeme), transcribed into the social structure of the three Czech villages. In the context of three case studies we focus on how the public space of these villages have changed and which elements from the past survived in this structure

Additional file 3: Table S2. of Novel factors of Anopheles gambiae haemocyte immune response to Plasmodium berghei infection

List of primers used in this study. (PDF 86 kb

Luciferase assays.

<p>(A) Transcriptional activation of <i>CEC1</i> promoter upon PGN challenge. The graph reports the z-scores calculated from the ratios of averaged RLU measurements after PGN and PBS treatments. (B and C) Transcriptional regulation of LRIM1 promoter (B) upon PGN challenge (z-scores calculated from the ratios of averaged RLU measurements after PGN and PBS treatments) and (C) in basal conditions (z-scores calculated from the RLU measured after PBS treatments). Positive hits are shown as open circles.</p

Schematic representation of the role of identified regulators in the <i>An. gambiae</i> hemocyte immune response.

<p>A total of 22 positive (green) and negative (red) regulators are shown. Dotted lines indicate modulations of <i>CEC1</i> and <i>LRIM1</i> gene expression upon PGN challenge, and solid lines describe the effect of genes on phagocytosis and basal expression of <i>LRIM1</i>.</p

<i>In vivo</i> phagocytosis assay.

<p>(A) Pictures of abdominal segments of adult female mosquitos injected with dsLacZ, dsAGAP004928 and dsAGAP008492 and then challenged with pHRodo <i>E. coli</i> bioparticles. Fluorescence and brightfield images were captured and hemocytes containing fluorescent bioparticles quantified. The experiment was repeated twice with 8–10 mosquitoes per treatment. Quantification of fluorescent bioparticles and statistical analysis were performed as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003145#s3" target="_blank">Materials and Methods</a>. (B) The graph shows the percentage of phagocytosis of the different KDs placing dsLacZ as reference. Mean values and standard errors are reported. Asterisks indicate statistical significance (**: 0,005LacZKD.</p

Optimization of Armored Spherical Tanks for Storage on the Lunar Surface

<div><p>Reverse genetics in the mosquito <em>Anopheles gambiae</em> by RNAi mediated gene silencing has led in recent years to an advanced understanding of the mosquito immune response against infections with bacteria and malaria parasites. We developed RNAi screens in <em>An. gambiae</em> hemocyte-like cells using a library of double-stranded RNAs targeting 109 genes expressed highly or specifically in mosquito hemocytes to identify novel regulators of the hemocyte immune response. Assays included phagocytosis of bacterial bioparticles, expression of the antimicrobial peptide CEC1, and basal and induced expression of the mosquito complement factor LRIM1. A cell viability screen was also carried out to assess dsRNA cytotoxicity and to identify genes involved in cell growth and survival. Our results identify 22 novel immune regulators, including proteins putatively involved in phagosome assembly and maturation (Ca²⁺ channel, v-ATPase and cyclin-dependent protein kinase), pattern recognition (fibrinogen-domain lectins and Nimrod), immune modulation (peptidase and serine protease homolog), immune signaling (Eiger and LPS-induced factor), cell adhesion and communication (Laminin B1 and Ninjurin) and immune homeostasis (Lipophorin receptor). The development of robust functional cell-based assays paves the way for genome-wide functional screens to study the mosquito immune response to infections with human pathogens.</p> </div

Bacterial phagocytosis screen.

<p>(A) Venn diagram showing the results of the z-score and ANOVA analyses of data obtained with the microplate reader phagocytosis assay. (B) Heat map depicting the performance in the microscopy imaging and <i>in vivo</i> phagocytosis assays of the 13 dsRNAs identified as positive hits with the microplate reader assay. Dark green, significant decrease of phagocytosis; green, decrease of phagocytosis; dark red, significant increase of phagocytosis; red, increase of phagocytosis; grey, similar to LacZ control; nd, not determined. (C) Microscopy imaging analysis: phagocytosis rates of cells at 2 and 20 h following bioparticles challenge when genes identified as modulators of phagocytosis by microplate reader assay are silenced. Data are shown as percent phagocytosis compared to ds<i>LacZ</i>-treated controls. (D) Examples of fluorescence microscopy images of Sua5.1* cells treated with dsRNAs or Cytochalasin D. Images were captured 20 h after challenge with pHRodo bioparticles. The graphs indicate the capacity of cells to uptake bioparticles following ds<i>LacZ</i>, ds<i>Cactus</i> and Cytochalasin D treatments at 2 and 20 h after challenge, as quantified by image analysis. Mean values of counted particles and standard errors are reported. Results from two experiments are shown. Asterisks indicate statistically significant differences between each KD and the dsLacZ-treated controls (*: P<0,05; **: 0,005</p

Cell growth and viability screen.

<p>(A) A fraction of the dsRNA hemocyte-specific library was screened twice by automated fluorescence microscopy. Fluorescent cells were automatically counted using protocols developed in Volocity Improvision and ImageJ software, as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003145#s3" target="_blank">Materials and Methods</a>. The ratios of dead cells (Sytox Green positive) over the total number of cells (Hoechst positive), and the standard deviation of replicates, are shown. (B) The entire dsRNA collection was screened using the Cell Titer Blue/Plate Reader method. The plot shows the z-score analysis of one representative set of experiments. A z-score threshold of at least 2 was chosen, and positive hits are shown as open circles. No dsRNA-treated samples and samples treated with 100 mM H₂O₂ are also indicated. Three biological replicates were performed.</p

Additional file 5: Figure S1. of Tissue-specific transcriptomes of Anisakis simplex (sensu stricto) and Anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity

Gene Ontology term distribution of differentially expressed genes (DEGs) in Anisakis simplex (s.s.). (JPEG 579 kb