Temperature Effects on Development of Three Cereal Aphid Parasitoids (Hymenoptera: Aphidiidae)

Temperature is an important climatological variable that influences the biology and ecology of insects. Poor climatic adaptation can limit the effectiveness of parasitic insects in biological control. Two exotic parasites (Syrian Diaeretiella rapae (M\u27Intosh) and Argentinean Aphidius colemani Viereck) imported for biological control of the Russian wheat aphid, Diuraphis noxia (Mordvilko), and one native parasite (Diaeretiella rapae) were reared in growth chambers in three fluctuating temperature regimes with average daily temperatures of 12, 18, and 24°C. Estimates of temperature thresholds for immature development were 3.3, 3.5, and 2.8°C, for Oklahoman D. rapae, Syrian D. rapae, and A. colemani, respectively. Estimates of thermal require- ments for development from egg to adult were 297, 278, and 301 degree-days for the three parasitoids. Dry weights of adults reared in different fluctuating temperature regimes did not differ significantly among sexes, but adults from regimes with low average temperatures of 12 and 18°C had significantly greater weights than those reared in a regime with an average temperature of 24°C. Results suggest that developmental response to temperature will not limit the effectiveness of the exotic parasites in biological control

Achievement Goal

Achievement goals are self-regulatory commitments that provide direction to individuals as they interpret and respond to competence-relevant situations. Four types of achievement goals have been the primary focus of the literature: Masteryapproach goals (master a task; improve over time), performance-approach goals (outperform others), mastery-avoidance goals (not fall short of mastering a task; not decline over time), and performance-avoidance goals (not be outperformed by others)

Clinician and carer moral concerns when caring for children who tube-feed.

Child healthcare can be vexed by moral concerns - this extends to the care of children who tube-feed. Children who tube-feed often receive care from family members and clinicians of various disciplines. Each brings expertise, experiences, values, and views to a situation, prioritising the child's needs while attending to those they deem important in potentially disparate ways. Their understanding of a situation is shaped by beliefs, feelings, and perceptions. How then are key decisions made about the care of a child who tube-feeds? This article explores clinicians' and carers' moral concerns when caring for children who tube-feed. Interviews with clinicians (n = 9) and carers (n = 9) clarified three findings: first, there are often disparate beliefs about the need for tube-feeding; second, tube-feeding can evoke strong emotions; and third, it can be difficult to normalise tube-feeding. This article demonstrates how challenges can emerge when relationships between clinicians and carers diverge. Furthermore, it establishes how an ethic of care can bring different interests together to bolster the relationships required to optimise feeding care and promote health outcomes among children who tube-feed and their carers. This requires improved dialogue between and among clinicians and carers to create shared understandings of what is, what should be, and how to benefit children who tube-feed

High-Throughput, Whole-Genome Sequencing

Since the completion of the Human Genome Project, research focusing on the consequence of known human genetic code has advanced by leaps and bounds. The development of personalized medicine, a field focused on enumerating the effects of individual genetic variations, termed SNPs, has become a reality for those researching the molecular basis of disease. With clinical correlates between genotype and prognosis becoming ever more common, the utility of personal genetic screening has become impossible to ignore. In this report, we present PennBio: a whole-genome sequencing company utilizing a novel single-molecule, real time sequencing-by-synthesis technology. Using unique zero-mode waveguides, which have revolutionized single-molecule detection, individual enzymes polymerizing novel phospholinked fluorescence labeled nucleotides can be observed as they sequence genomic template DNA. Modern optical techniques record these fragmented sequences, which are then analyzed by highly efficient alignment algorithms. A personal genomic code will ultimately allow consumers to be aware of their genetic predispositions as the medical community continues to discover them

Identification and characterization of small compound inhibitors of human FATP2

Fatty acid transport proteins (FATPs) are bifunctional proteins, which transport long chain fatty acids into cells and activate very long chain fatty acids by esterification with coenzyme A. In an effort to understand the linkage between cellular fatty acid transport and the pathology associated with excessive accumulation of exogenous fatty acids, we targeted FATP-mediated fatty acid transport in a high throughput screen of more than 100,000 small diverse chemical compounds in yeast expressing human FATP2 (hsFATP2). Compounds were selected for their ability to depress the transport of the fluorescent long chain fatty acid analogue, C1-BODIPY-C12. Among 234 hits identified in the primary screen, 5 compounds, each representative of a structural class, were further characterized in the human Caco-2 and HepG2 cell lines, each of which normally expresses FATP2, and in 3T3-L1 adipocytes, which do not. These compounds were effective in inhibiting uptake with IC50s in the low micromolar range in both Caco-2 and HepG2 cells. Inhibition of transport was highly specific for fatty acids and there were no effects of these compounds on cell viability, trans-epithelial electrical resistance, glucose transport, or long chain acyl-CoA synthetase activity. The compounds were less effective when tested in 3T3-L1 adipocytes suggesting selectivity of inhibition. These results suggest fatty acid transport can be inhibited in a FATP-specific manner without causing cellular toxicity

Mechanisms of Manganese-Assisted Nonradiative Recombination in Cd(Mn)Se/Zn(Mn)Se Quantum Dots

Mechanisms of nonradiative recombination of electron-hole complexes in Cd(Mn)Se/Zn(Mn)Se quantum dots accompanied by interconfigurational excitations of Mn$^{2+}$ ions are analyzed within the framework of single electron model of deep {\it 3d}-levels in semiconductors. In addition to the mechanisms caused by Coulomb and exchange interactions, which are related because of the Pauli principle, another mechanism due to {\it sp-d} mixing is considered. It is shown that the Coulomb mechanism reduces to long-range dipole-dipole energy transfer from photoexcited quantum dots to Mn$^{2+}$ ions. The recombination due to the Coulomb mechanism is allowed for any states of Mn$^{2+}$ ions and {\it e-h} complexes. In contrast, short-range exchange and ${\it sp-d}$ recombinations are subject to spin selection rules, which are the result of strong {\it lh-hh} splitting of hole states in quantum dots. Estimates show that efficiency of the {\it sp-d} mechanism can considerably exceed that of the Coulomb mechanism. The phonon-assisted recombination and processes involving upper excited states of Mn$^{2+}$ ions are studied. The increase in PL intensity of an ensemble of quantum dots in a magnetic field perpendicular to the sample growth plane observed earlier is analyzed as a possible manifestation of the spin-dependent recombination.Comment: 14 pages, 2 figure

Connecting through research: A collaborative autoethnography of a positive culture in an inter-institutional research group

The LUCARA (Lincoln University, University of Canterbury, and Ara Te Pūkenga) group is a thriving research group based in Ōtautahi Christchurch, New Zealand. The group consists of nine academics in sport and exercise science, health, and nutrition at the three tertiary institutions. The majority of the group members had previously been involved in collaborative research for over 10 years. Initially, the relationships during this time were largely transactional, for example, editorial feedback, funding support, data collection, and statistical support. However, in the last two-and-half years, the group has matured, and relationships have deepened with weekly meetings, connections, and partnerships, which has produced a large increase in the number of collaborations between researchers, sharing of resources, and hence increased research outputs. This narrative explores the organic researcher-led growth in the group, and uses the theme of connections to gain an understanding of how this culture has blossomed over a relatively short time in a sport and allied health research setting

Radio Continuum and Recombination Line Study of UC HII Regions with Extended Envelopes

We have carried out 21 cm radio continuum observations of 16 UC HII regions using the VLA (D-array) in search of associated extended emission. We have also observed H76$_\alpha$ recombination line towards all the sources and He76$_\alpha$ line at the positions with strong H76$_\alpha$ line emission. The UC HII regions have simple morphologies and large (>10) ratios of single-dish to VLA fluxes. Extended emission was detected towards all the sources. The extended emission consists of one to several compact components and a diffuse extended envelope. All the UC HII regions but two are located in the compact components, where the UC HII regions always correspond to their peaks. The compact components with UC HII regions are usually smaller and denser than those without UC HII regions. Our recombination line observations indicate that the ultracompact, compact, and extended components are physically associated. The UC HII regions and their associated compact components are likely to be ionized by the same sources on the basis of the morphological relations mentioned above. This suggests that almost all of the observed UC HII regions are not `real' UC HII regions and that their actual ages are much greater than their dynamical age (<10000 yr). We find that most of simple UC HII regions previously known have large ratios of single-dish to VLA fluxes, similar to our sources. Therefore, the `age problem' of UC HII regions does not seem to be as serious as earlier studies argued. We present a simple model that explains extended emission around UC HII regions. Some individual sources are discussed.Comment: 29 pages, 28 postscript figures, Accepted for publication in Ap

Paediatric tube-feeding: An agenda for care improvement and research.

This article presents an agenda to improve the care and wellbeing of children with paediatric feeding disorder who require tube feeding (PFD-T). PFD-T requires urgent attention in practice and research. Priorities include: routine collection of PFD-T data in health-care records; addressing the tube-feeding lifecycle; and reducing the severity and duration of disruption caused by PFD-T where possible. This work should be underpinned by principles of involving, respecting and connecting families