Biased gene expression in early honeybee larval development

Background: Female larvae of the honeybee (Apis mellifera) develop into either queens or workers depending on nutrition. This nutritional stimulus triggers different developmental trajectories, resulting in adults that differ from each other in physiology, behaviour and life span. Results: To understand how these trajectories are established we have generated a comprehensive atlas of gene expression throughout larval development. We found substantial differences in gene expression between worker and queen-destined larvae at 6 hours after hatching. Some of these early changes in gene expression are maintained throughout larval development, indicating that caste-specific developmental trajectories are established much earlier than previously thought. Within our gene expression data we identified processes that potentially underlie caste differentiation. Queen-destined larvae have higher expression of genes involved in transcription, translation and protein folding early in development with a later switch to genes involved in energy generation. Using RNA interference, we were able to demonstrate that one of these genes, hexamerin 70b, has a role in caste differentiation. Both queen and worker developmental trajectories are associated with the expression of genes that have alternative splice variants, although only a single variant of a gene tends to be differentially expressed in a given caste. Conclusions: Our data, based on the biases in gene expression early in development together with published data, supports the idea that caste development in the honeybee consists of two phases; an initial biased phase of development, where larvae can still switch to the other caste by differential feeding, followed by commitment to a particular developmental trajectory

The magnetar emission in the IR band: the role of magnetospheric currents

There is a general consensus about the fact that the magnetar scenario provides a convincing explanation for several of the observed properties of the Anomalous X-ray Pulsars and the Soft Gamma Repeaters. However, the origin of the emission observed at low energies is still an open issue. We present a quantitative model for the emission in the optical/infrared band produced by curvature radiation from magnetospheric charges, and compare results with current magnetars observations.Comment: 6 Pages, 2 Figures. Astrophysics and Space Science, in press. Proceedings of the ICREA Workshop on The High-Energy Emission from Pulsars and their Systems, Sant Cugat, April 12-16 201

The effect of the systemic inflammatory response on plasma vitamin 25 (OH) D concentrations adjusted for albumin

<b>Aim</b><p></p> To examine the relationship between plasma 25(OH)D, CRP and albumin concentrations in two patient cohorts.<p></p> <b>Methods</b><p></p> 5327 patients referred for nutritional assessment and 117 patients with critical illness were examined. Plasma 25 (OH) D concentrations were measured using standard methods. Intra and between assay imprecision was <10%.<p></p> <b>Result</b><p></p> In the large cohort, plasma 25 (OH) D was significantly associated with CRP (rs = −0.113, p<0.001) and albumin (rs = 0.192, p<0.001). 3711 patients had CRP concentrations ≤10 mg/L; with decreasing albumin concentrations ≥35, 25–34 and <25 g/l, median concentrations of 25 (OH) D were significantly lower from 35 to 28 to 14 nmol/l (p<0.001). This decrease was significant when albumin concentrations were reduced between 25–34 g/L (p<0.001) and when albumin <25 g/L (p<0.001). 1271 patients had CRP concentrations between 11–80 mg/L; with decreasing albumin concentrations ≥35, 25–34 and <25 g/l, median concentrations of 25 (OH) D were significantly lower from 31 to 24 to 19 nmol/l (p<0.001). This decrease was significant when albumin concentration were 25–34 g/L (p<0.001) and when albumin <25 g/L (p<0.001). 345 patients had CRP concentrations >80 mg/L; with decreasing albumin concentrations ≥35, 25–34 and <25 g/l, median concentrations of 25 (OH) D were not significantly altered varying from 19 to 23 to 23 nmol/l. Similar relationships were also obtained in the cohort of patients with critical illness.<p></p> <b>Conclusion</b><p></p> Plasma concentrations of 25(OH) D were independently associated with both CRP and albumin and consistent with the systemic inflammatory response as a major confounding factor in determining vitamin D status.<p></p&gt

Young neutron stars with soft gamma ray emission and anomalous X-ray pulsar

The observational properties of Soft Gamma Repeaters and Ano\-malous X-ray Pulsars (SGR/AXP) indicate to necessity of the energy source different from a rotational energy of a neutron star. The model, where the source of the energy is connected with a magnetic field dissipation in a highly magnetized neutron star (magnetar) is analyzed. Some observational inconsistencies are indicated for this interpretation. The alternative energy source, connected with the nuclear energy of superheavy nuclei stored in the nonequilibrium layer of low mass neutron star is discussed.Comment: 29 pages, 13 figures, Springer International Publishing Switzerland 2016 A.W. Alsabti, P. Murdin (eds.), Handbook of Supernova

Magnetized Domain Walls in the Deconfined Sakai-Sugimoto Model at Finite Baryon Density

The magnetized pure pion gradient ($\mathcal{5}\phi$) phase in the deconfined Sakai-Sugimoto model is explored at zero and finite temperature. We found that the temperature has very small effects on the phase. The thermodynamical properties of the phase shows that the excitations behave like a scalar solitonic free particles. By comparing the free energy of the pion gradient phase to the competing multiquark-pion gradient (MQ-$\mathcal{5}\phi$) phase, it becomes apparent that the pure pion gradient is less thermodynamically preferred than the MQ-$\mathcal{5}\phi$ phase. However, in the parameter space where the baryonic chemical potential is smaller than the onset value of the multiquark, the dominating magnetized nuclear matter is the pion gradient phase.Comment: 20 pages, 9 figure

Nuclei in Strongly Magnetised Neutron Star Crusts

We discuss the ground state properties of matter in outer and inner crusts of neutron stars under the influence of strong magnetic fields. In particular, we demonstrate the effects of Landau quantization of electrons on compositions of neutron star crusts. First we revisit the sequence of nuclei and the equation of state of the outer crust adopting the Baym, Pethick and Sutherland (BPS) model in the presence of strong magnetic fields and most recent versions of the theoretical and experimental nuclear mass tables. Next we deal with nuclei in the inner crust. Nuclei which are arranged in a lattice, are immersed in a nucleonic gas as well as a uniform background of electrons in the inner crust. The Wigner-Seitz approximation is adopted in this calculation and each lattice volume is replaced by a spherical cell. The coexistence of two phases of nuclear matter - liquid and gas, is considered in this case. We obtain the equilibrium nucleus corresponding to each baryon density by minimizing the free energy of the cell. We perform this calculation using Skyrme nucleon-nucleon interaction with different parameter sets. We find nuclei with larger mass and charge numbers in the inner crust in the presence of strong magnetic fields than those of the zero field case for all nucleon-nucleon interactions considered here. However, SLy4 interaction has dramatic effects on the proton fraction as well as masses and charges of nuclei. This may be attributed to the behaviour of symmetry energy with density in the sub-saturation density regime. Further we discuss the implications of our results to shear mode oscillations of magnetars.Comment: presented in "Exciting Physics Symposium" held in Makutsi, South Africa in November, 2011 and to be published in a book by Springer Verla

Magnetars and pulsars: a missing link

There is growing evidence that soft gamma-ray repeaters (SGRs) and anomalous X-ray pulsars (AXPs) are isolated neutron stars with superstrong magnetic fields, i.e., magnetars, marking them a distinguished species from the conventional species of spindown-powered isolated neutron stars, i.e., radio pulsars. The current arguments in favor of the magnetar interpretation of SGR/AXP phenomenology will be outlined, and the two energy sources in magnetars, i.e. a magnetic dissipation energy and a spindown energy, will be reviewed. I will then discuss a missing link between magnetars and pulsars, i.e., lack of the observational evidence of the spindown-powered behaviors in known magnetars. Some recent theoretical efforts in studying such behaviors will be reviewed along with some predictions testable in the near future.Comment: Invited talk at the Sixth Pacific Rim Conference on Stellar Astrophysics, a tribute to Helmut A. Abt, July 11-17, 2002, Xi'an. To appear in the proceedings (eds. K. S. Cheng, K. C. Leung & T. P. Li

Participatory development of decision support systems: which features of the process lead to improved uptake and better outcomes?

Decision support systems (DSSs) are important in decision-making environments with conflicting interests. Many DSSs developed have not been used in practice. Experts argue that these tools do not respond to real user needs and that the inclusion of stakeholders in the development process is the solution. However, it is not clear which features of participatory development of DSSs result in improved uptake and better outcomes. A review of papers, reporting on case studies where DSSs and other decision tools (information systems, software and scenario tools) were developed with elements of participation, was carried out. The cases were analysed according to a framework created as part of this research; it includes criteria to evaluate the development process and the outcomes. Relevant aspects to consider in the participatory development processes include establishing clear objectives, timing and location of the process; keeping discussions on track; favouring participation and interaction of individuals and groups; and challenging creative thinking of the tool and future scenarios. The case studies that address these issues show better outcomes; however, there is a large degree of uncertainty concerning them because developers have typically neither asked participants about their perceptions of the processes and resultant tools nor have they monitored the use and legacy of the tools over the long term.The authors would like to thank COST Action FP0804-Forest Management Decision Support Systems (FORSYS) for financing a three month Short-Term Scientific Mission (STSM) in Forest Research (Roslin, UK) in 2012, making possible this research; Spanish Ministry of Economy and Competitiveness for supporting the project Multicriteria Techniques and Participatory Decision-Making for Sustainable Management (Ref. ECO2011-27369) where the leading author is involved; and the Regional Ministry of Education, Culture and Sports (Valencia, Spain) for financing a research fellowship (Ref. ACIF/2010/248).Valls Donderis, P.; Ray, D.; Peace, A.; Stewart, A.; Lawrence, A.; Galiana, F. (2013). Participatory development of decision support systems: which features of the process lead to improved uptake and better outcomes?. Scandinavian Journal of Forest Research. 29(1):71-83. https://doi.org/10.1080/02827581.2013.837950S7183291Arnstein, S. R. (1969). A Ladder Of Citizen Participation. Journal of the American Institute of Planners, 35(4), 216-224. doi:10.1080/01944366908977225Atwell, R. C., Schulte, L. A., & Westphal, L. M. (2011). Tweak, Adapt, or Transform: Policy Scenarios in Response to Emerging Bioenergy Markets in the U.S. Corn Belt. Ecology and Society, 16(1). doi:10.5751/es-03854-160110Barac, A., Kellner, K., & De Klerk, N. (2004). Land User Participation in Developing a Computerised Decision Support System for Combating Desertification. Environmental Monitoring and Assessment, 99(1-3), 223-231. doi:10.1007/s10661-004-4022-6Bennet, A., & Bennet, D. (2008). The Decision-Making Process in a Complex Situation. Handbook on Decision Support Systems 1, 3-20. doi:10.1007/978-3-540-48713-5_1Blackstock, K. L., Kelly, G. J., & Horsey, B. L. (2007). Developing and applying a framework to evaluate participatory research for sustainability. Ecological Economics, 60(4), 726-742. doi:10.1016/j.ecolecon.2006.05.014Breuer, N. E., Cabrera, V. E., Ingram, K. T., Broad, K., & Hildebrand, P. E. (2007). AgClimate: a case study in participatory decision support system development. Climatic Change, 87(3-4), 385-403. doi:10.1007/s10584-007-9323-7Bunch, M. J., & Dudycha, D. J. (2004). Linking conceptual and simulation models of the Cooum River: collaborative development of a GIS-based DSS for environmental management. Computers, Environment and Urban Systems, 28(3), 247-264. doi:10.1016/s0198-9715(03)00021-8Byrne, E., & Sahay, S. (2007). Participatory design for social development: A South African case study on community-based health information systems. Information Technology for Development, 13(1), 71-94. doi:10.1002/itdj.20052Cain, J. ., Jinapala, K., Makin, I. ., Somaratna, P. ., Ariyaratna, B. ., & Perera, L. . (2003). Participatory decision support for agricultural management. A case study from Sri Lanka. Agricultural Systems, 76(2), 457-482. doi:10.1016/s0308-521x(02)00006-9Chakraborty, A. (2011). Enhancing the role of participatory scenario planning processes: Lessons from Reality Check exercises. Futures, 43(4), 387-399. doi:10.1016/j.futures.2011.01.004Cinderby, S., Bruin, A. de, Mbilinyi, B., Kongo, V., & Barron, J. (2011). Participatory geographic information systems for agricultural water management scenario development: A Tanzanian case study. Physics and Chemistry of the Earth, Parts A/B/C, 36(14-15), 1093-1102. doi:10.1016/j.pce.2011.07.039Drew, C. H., Nyerges, T. L., & Leschine, T. M. (2004). Promoting Transparency of Long‐Term Environmental Decisions: The Hanford Decision Mapping System Pilot Project. Risk Analysis, 24(6), 1641-1664. doi:10.1111/j.0272-4332.2004.00556.xDriedger, S. M., Kothari, A., Morrison, J., Sawada, M., Crighton, E. J., & Graham, I. D. (2007). Using participatory design to develop (public) health decision support systems through GIS. International Journal of Health Geographics, 6(1), 53. doi:10.1186/1476-072x-6-53Evers, M. (2008). An analysis of the requirements for DSS on integrated river basin management. Management of Environmental Quality: An International Journal, 19(1), 37-53. doi:10.1108/14777830810840354Iivari, N. (2011). Participatory design in OSS development: interpretive case studies in company and community OSS development contexts. Behaviour & Information Technology, 30(3), 309-323. doi:10.1080/0144929x.2010.503351Innes, J. E., & Booher, D. E. (1999). Consensus Building and Complex Adaptive Systems. Journal of the American Planning Association, 65(4), 412-423. doi:10.1080/01944369908976071Jakku, E., & Thorburn, P. J. (2010). A conceptual framework for guiding the participatory development of agricultural decision support systems. Agricultural Systems, 103(9), 675-682. doi:10.1016/j.agsy.2010.08.007Jessel, B., & Jacobs, J. (2005). Land use scenario development and stakeholder involvement as tools for watershed management within the Havel River Basin. Limnologica, 35(3), 220-233. doi:10.1016/j.limno.2005.06.006Kautz, K. (2011). Investigating the design process: participatory design in agile software development. Information Technology & People, 24(3), 217-235. doi:10.1108/09593841111158356Kowalski, K., Stagl, S., Madlener, R., & Omann, I. (2009). Sustainable energy futures: Methodological challenges in combining scenarios and participatory multi-criteria analysis. European Journal of Operational Research, 197(3), 1063-1074. doi:10.1016/j.ejor.2007.12.049Lawrence, A. (2006). ‘No Personal Motive?’ Volunteers, Biodiversity, and the False Dichotomies of Participation. Ethics, Place & Environment, 9(3), 279-298. doi:10.1080/13668790600893319Mao, J., & Song, W. (2008). Empirical study of distinct features and challenges of joint development of information systems: The case of ABC bank. Tsinghua Science and Technology, 13(3), 414-419. doi:10.1016/s1007-0214(08)70066-xMenzel, S., Nordström, E.-M., Buchecker, M., Marques, A., Saarikoski, H., & Kangas, A. (2012). Decision support systems in forest management: requirements from a participatory planning perspective. European Journal of Forest Research, 131(5), 1367-1379. doi:10.1007/s10342-012-0604-yMoote, M. A., Mcclaran, M. P., & Chickering, D. K. (1997). RESEARCH: Theory in Practice: Applying Participatory Democracy Theory to Public Land Planning. Environmental Management, 21(6), 877-889. doi:10.1007/s002679900074Peleg, M., Shachak, A., Wang, D., & Karnieli, E. (2009). Using multi-perspective methodologies to study users’ interactions with the prototype front end of a guideline-based decision support system for diabetic foot care. International Journal of Medical Informatics, 78(7), 482-493. doi:10.1016/j.ijmedinf.2009.02.008Pretty, J. N. (1995). Participatory learning for sustainable agriculture. World Development, 23(8), 1247-1263. doi:10.1016/0305-750x(95)00046-fReed MS. 2008. Stakeholder participation for environmental management: a literature review. Sustainability Research Institute, School of Earth and Environment, University of Leeds.Reed, M. S., & Dougill, A. J. (2010). Linking degradation assessment to sustainable land management: A decision support system for Kalahari pastoralists. Journal of Arid Environments, 74(1), 149-155. doi:10.1016/j.jaridenv.2009.06.016Rowe, G., & Frewer, L. J. (2000). Public Participation Methods: A Framework for Evaluation. Science, Technology, & Human Values, 25(1), 3-29. doi:10.1177/016224390002500101Schielen, R. M. J., & Gijsbers, P. J. A. (2003). DSS-large rivers: developing a DSS under changing societal requirements. Physics and Chemistry of the Earth, Parts A/B/C, 28(14-15), 635-645. doi:10.1016/s1474-7065(03)00109-8Sheppard, S. R. J., & Meitner, M. (2005). Using multi-criteria analysis and visualisation for sustainable forest management planning with stakeholder groups. Forest Ecology and Management, 207(1-2), 171-187. doi:10.1016/j.foreco.2004.10.032Thursky, K. A., & Mahemoff, M. (2007). User-centered design techniques for a computerised antibiotic decision support system in an intensive care unit. International Journal of Medical Informatics, 76(10), 760-768. doi:10.1016/j.ijmedinf.2006.07.011Webler, S. T., Thomas. (1999). Voices from the Forest: What Participants Expect of a Public Participation Process. Society & Natural Resources, 12(5), 437-453. doi:10.1080/089419299279524Van Meensel, J., Lauwers, L., Kempen, I., Dessein, J., & Van Huylenbroeck, G. (2012). Effect of a participatory approach on the successful development of agricultural decision support systems: The case of Pigs2win. Decision Support Systems, 54(1), 164-172. doi:10.1016/j.dss.2012.05.002Von Geibler, J., Kristof, K., & Bienge, K. (2010). Sustainability assessment of entire forest value chains: Integrating stakeholder perspectives and indicators in decision support tools. Ecological Modelling, 221(18), 2206-2214. doi:10.1016/j.ecolmodel.2010.03.02

Pelvic mass associated with raised CA 125 for benign condition: a case report

<p>Abstract</p> <p>Background</p> <p>Raised CA 125 with associated pelvic mass is highly suggestive of ovarian malignancy, but there are various other benign conditions that can be associated with pelvic mass and a raised CA 125.</p> <p>Case presentation</p> <p>We present a case of 19 year old, Caucasian British woman who presented initially with sudden onset right sided iliac fossa pain and on imaging was found to have 9.8 × 4.5 cm complex cystic mass in right adnexa with a raised CA 125 of 657, which was initially thought to be highly suspicious of cancer but was subsequently found to be due to pelvic inflammatory disease on histology.</p> <p>Conclusion</p> <p>This case highlights the fact that though a pelvic mass with raised CA 125 is highly suggestive of malignancy, pelvic inflammatory disease should always be considered as a differential diagnosis especially in a young patient and a thorough sexual history and screening for pelvic infection should always be carried out in these patients.</p

Human mass balance study of the novel anticancer agent ixabepilone using accelerator mass spectrometry

Ixabepilone (BMS-247550) is a semi-synthetic, microtubule stabilizing epothilone B analogue which is more potent than taxanes and has displayed activity in taxane-resistant patients. The human plasma pharmacokinetics of ixabepilone have been described. However, the excretory pathways and contribution of metabolism to ixabepilone elimination have not been determined. To investigate the elimination pathways of ixabepilone we initiated a mass balance study in cancer patients. Due to autoradiolysis, ixabepilone proved to be very unstable when labeled with conventional [14C]-levels (100 μCi in a typical human radio-tracer study). This necessitated the use of much lower levels of [14C]-labeling and an ultra-sensitive detection method, Accelerator Mass Spectrometry (AMS). Eight patients with advanced cancer (3 males, 5 females; median age 54.5 y; performance status 0–2) received an intravenous dose of 70 mg, 80 nCi of [14C]ixabepilone over 3 h. Plasma, urine and faeces were collected up to 7 days after administration and total radioactivity (TRA) was determined using AMS. Ixabepilone in plasma and urine was quantitated using a validated LC-MS/MS method. Mean recovery of ixabepilone-derived radioactivity was 77.3% of dose. Fecal excretion was 52.2% and urinary excretion was 25.1%. Only a minor part of TRA is accounted for by unchanged ixabepilone in both plasma and urine, which indicates that metabolism is a major elimination mechanism for this drug. Future studies should focus on structural elucidation of ixabepilone metabolites and characterization of their activities