14 research outputs found
Partial purification of a serum factor that causes necrosis of tumors.
Full text linkTumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial endotoxin. Sera from normal mice, endotoxin-treated mice, and mice infected with bacillus Calmette-Guérin do not contain this factor. A 20- to 30-fold purification of the serum factor has been achieved by (NH4)2SO4 fractionation, Sephadex G-100 and G-200 gel filtration, and preparative polyacrylamide electrophoresis. Tumor necrosis factor is not bacterial endotoxin. It migrates with alpha-globulins, is made up of at least four subunits, and has a molecular weight of about 150,000. The active factor is a glycoprotein that contains sialic acid and galactosamine
Nicotinamide adenine dinucleotide glycohydrolases from ehrlich ascites tumor cell nuclei, isolation, partial purification, and properties.
No full textA relation between pyridine nucleotide-dependent dehydrogenase activity and nicotinamide adenine dinucleotide glycohydrolase in ehrlich ascites tumor cells.
No full textRelationship of the nicotinamide adenine dinucleotide glycohydrolase activity to nicotinamide adenine dinucleotide content and rate of proliferation of ehrlich ascites tumor cells.
No full textConsequences of increased nadase activity, nad-specific dehydrogenase activity in ehrlich ascites cells. Abstr.
No full text
Murine tumor necrosis-inducing factor: purification and effects on myelomonocytic leukemia cells.
Get PDFAction of tumor necrosis factor (tnf) on mouse myelomonocytic leukemia (wehi/3) in vitro and in vivo. Abstr.
No full textMurine tumor necrosis-inducing factor: purification and effects on myelomonocytic leukemia cells.
No full textPartial purification of a serum factor that causes necrosis of tumors.
No full textTumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial endotoxin. Sera from normal mice, endotoxin-treated mice, and mice infected with bacillus Calmette-Guérin do not contain this factor. A 20- to 30-fold purification of the serum factor has been achieved by (NH4)2SO4 fractionation, Sephadex G-100 and G-200 gel filtration, and preparative polyacrylamide electrophoresis. Tumor necrosis factor is not bacterial endotoxin. It migrates with alpha-globulins, is made up of at least four subunits, and has a molecular weight of about 150,000. The active factor is a glycoprotein that contains sialic acid and galactosamine
