High osmolar contrast medium causes mild oxidation in liver, bladder, and ovary tissues from rats: vitamin C has protective role

The purpose of this study was to investigate effects of ionic highosmolar contrast medium on oxidative metabolism in liver, urinary bladder, and ovary tissues and to obtain information about possible protective effects of vitamin C. Twenty-one female rats, 14 weeks old, were used in this study. They were divided into three groups of seven rats: Sham (group I), contrast (group II), contrast ? vitamin C (group III). Vitamin C was given orally to the animals in group III during the study period. On the fifth day, contrast medium was given via intravenous infusion as a single dose to the animals in groups II and III. On the sixth day of the study, the animals were killed with anesthesia by ketamine hydrochloride. Then, their liver, bladder, and ovary tissues were removed to measure analyses parameters. Our results suggested that contrast medium led to some increases in malondialdehyde levels in the liver, bladder, and ovary tissues and that vitamin C prevented these increases in the tissues. Nitric oxide level also was found to increase in the contrast-treated animals and vitamin C prevented this increase in the liver tissue. Ionic high-osmolar contrast medium leads to weak oxidant stress in rat liver, bladder, and ovary tissues, and vitamin C prevents this oxidant stress

The effect of diosmin on pancreatic injury induced by hepatic ischemia reperfusion in rats

Abstract: Background: Hepatic ischemia-reperfusion causes histological injury to the pancreatic cells during transplantation, trauma and emergency surgery. We investigated the effects of diosmin, a phlebotrophic drug with antioxidant and antiinfl ammatory effects, on pancreatic injury in the experimental liver ischemia-reperfusion model. Materials and methods: Forty rats were divided into the four groups: sham (Group 1), control (Group 2), peroperative diosmin (50 mg/kg) treatment (Group 3) and preoperative 10-day diosmin (50 mg/kg) treatment group (Group 4). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 60 min and then reperfusing the liver for 90 min. At the end of the procedures, blood and pancreas tissue samples were obtained for biochemical and histopathological assessment. Results: According to the results of liver function tests, amylase and the plasma and pancreatic tissue oxidative stress parameters, there was a signifi cant difference between the control and other groups. Histopathologically; the specimens of the Group 2 showed specifi c morphological abnormalities. The groups 3 and 4 showed the pancreas histomorphology similar to the sham group. Pathological scores were signifi cantly different between the Group 2 and other groups. Conclusions: Diosmin can be administered for a protection of destructive effects of hepatic ischemia–reperfusion injury on pancreas both emergent and elective hepatic surgical operations in which possible ischemic periods were expecte

<b>Effect of Garlic and Red Clover extracts on adenosine deaminase enzyme activities in cancerous and non-cancerous human liver tissues</b>

97-101Possible effects of aqueous garlic (Allium sativum Linn.) and red clover (Trifolium pratense Linn.) extracts on adenosine deaminase (ADA) activities were investigated in cancerous and non-cancerous human liver tissues and results were compared with those of Fludarabine, a known ADA inhibitor. Ten cancerous and 10 non-cancerous adjacent liver tissues were obtained from patients with metastatic type liver cancer by surgical operations. Kinetic analyses were carried out to establish Vmax and Km values of the reaction catalyzed by ADA in the presence or absence of inhibitor. ADA activity was found to be lower in the cancerous tissues compared with non-cancerous tissues. Aqueous garlic and red clover extracts induced significant inhibition ADA activity in both the tissues. Inhibition percentage was relatively higher in the cancerous tissues as compared with non-cancerous tissues. Furthermore, we observed that inhibition percentage induced by garlic and red clover extracts were higher than those of Fludarabine at the concentrations studied

Simultaneous uroflowmetry and urinalysis with single specimen – A prospective evaluation of automatic urine strip analyzer of ORUBA INALYS: Uroflowmetry-urinalysis combined device

Objective: To evaluate the consistency of the urinalysis results performed with the ORUBA INALYS device, (Oruba, Ankara, Turkey) which can perform urinalysis and uroflowmetry simultaneously, with the analysis results performed with the SYSMEX UC3500 automated urine chemistry analyzer (Sysmex, Kobe, Japan). Material and methods: In this prospective study, urinalysis of 50 male patients with lower urinary tract symptoms were evaluated. The parameters of glucose, pH, urobilinogen, bilirubin and ketone, leukocyte, protein, and blood were measured with ORUBA INALYS, and the same urine specimens collected from ORUBA INALYS by a special setup were sent to the laboratory for urinalysis with Sysmex UC-3500 to assess the concordance of the results between two devices. Results: Urinalysis results in ORUBA INALYS device in terms of glucose, pH, urobilinogen, bilirubin, and ketone parameters were shown to achieve 100% agreement within ±1 category with SYSMEX UC3500 whereas these values were slightly decreased to 88%, 96%, and 98% for leukocyte, protein, and blood, respectively. Among the calculable weighted kappa values for the test parameters, the highest value was found for glucose and followed by blood, pH, leukocyte, and specific gravity respectively. Conclusion: Significant consistency of the urinalysis results obtained from ORUBA INALYS with those obtained from device SYSMEX UC3500 shows the reliability of the urinalysis performed with ORUBA INALYS. ORUBA INALYS could minimize costs and workload, provide time save and reduce plastic waste

The effect of 2100 MHz radiofrequency radiation of a 3G mobile phone on the parotid gland of rats

11th International Congress of Otorhinolaryngology and Head and Neck Surgery -- APR 17-19, 2014 -- Ankara, TURKEYDevrim, Erdinc/0000-0001-7326-5104; Tomruk, Arin/0000-0002-7600-0811; Devrim, Erdinc/0000-0001-7326-5104; Devrim, Erdinc/0000-0001-7326-5104; UNSAL, VELID/0000-0003-1415-0563; Ozgur-Buyukatalay, Elcin/0000-0001-6428-918XWOS: 000346756300007PubMed: 25456509Purpose: We aimed to evaluate the effect of 2100 MHz radiofrequency radiation on the parotid gland of rats in short and relatively long terms. Material and methods: Thirty Wistar albino rats were divided into four groups. Groups A and B served as the control groups (for 10 days and 40 days, respectively), and each group included six rats. Groups C and D were composed of nine rats each, and they were the exposure groups. The rats were exposed to 2100 MHz radiofrequency radiation emitted by a generator, simulating a third generation mobile phone for 6 hours/day, 5 days/week, for 10 or 40 days. Following exposure, the rats were sacrificed and parotid glands were removed. Histopathological and biochemical examinations were performed. Results: Although there were no histopathological changes in the control groups except for two animals in group A and three animals in group B, the exposure groups C (10 days) and D (40 days) showed numerous histopathological changes regarding salivary gland damage including acinar epithelial cells, interstitial space, ductal system, vascular system, nucleus, amount of cytoplasm and variations in cell size. The histopathological changes were more prominent in group D compared to group C. There was statistically significant different parameter regarding variation in cell size between the groups B and D (p = 0.036). Conclusion: The parotid gland of rats showed numerous histopathological changes after exposure to 2100 MHz radiofrequency radiation, both in the short and relatively long terms. Increased exposure duration led to an increase in the histopathological changes. (C) 2015 Elsevier Inc. All rights reserved

Effects of crt monitor-emitted radiation in rat tissues: preventive effect of vitamin C

Objective: To investigate the effects of CRT (cathode ray tube)-monitor-emitted radiation on the oxidant/antioxidant status in kidney, liver, heart, brain tissues of rats and to observe the histo pathological findings of these tissues, and to examine any protective role of vitamin C supplementation. Material and Method: The study carried out on 40 Wistar albino adult female rats. There were 10 animals in each four group (control, vitamin C, computer; and computer plus vitamin C). The computer and computer plus vitamin C groups were exposed to computer monitors while the other groups were not. Vitamin C was administered 250 mg/kg/day orally. In the kidney, liver, heart, and brain tissues, malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities were measured spectrophotometrically. In addition, histopathological examination is carried out. Results: In the kidney tissues, MDA levels significantly increased in the computer group compared with the computer plus vitamin C group and the control group (p<0.05). Histomorphologic changes were observed in the kidney and liver tissues of the computer group while there were no alterations in other tissues. Conclusion: The results of this study suggest that CRT-monitor-emitted radiation leads to oxidative stress and cellular changes in kidney and liver tissues and the antioxidant supplementation like vitamin C could prevent these possible oxidative effects

Diosmin ameliorates intestinal injury induced by hepatic ischemia reperfusion in rats

Background: Hepatic ischemia-reperfusion causes histologic injury to the intestinal mucosa. We investigated the effects of diosmin, a phelobotrophic drug with antioxidant and antiinflammatory effects, on intestinal injury in the experimental liver ischemia-reperfusion model. Materials and methods: Fourty rats were divided into four groups: sham group (Group 1), control group (Group 2), perop diosmin group (50 mg/kg) treatment group (Group 3) and preop 10-day diosmin (50 mg/kg) treatment group (Group 4). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 60 min and then reperfusing the liver for 90 min. At the end of procedures, blood and ileum tissue samples were obtained for biochemical and histopathological assessments. Results: According to the results of liver function tests (AST, ALT and LDH) there was a significant difference between the control and other groups (p<0.001 for all). According to the plasma and ileum oxidative stress parameters (MDA, GSH-Px and XO), there was a significant difference between the control and other groups (p<0.05 for all). Histopathologically; the specimens in Group 2 showed specific morphological abnormalities (the epithelial lining of the apical surface of villi was degenerated and desquamated to the lumen). Group 3 and 4 showed Heal histomorphology similar to the sham group. Pathological scores were significantly different between Group 2 and other groups. Conclusions: Diosmin can be administered for protection from destructive effects of hepatic ischemia-reperfusion injury on intestine in both emergent and elective hepatic surgical operations in which the possible ischemic periods are expected (Tab. 4, Fig. 1, Ref. 39). Full Text in free PDF www.bmj.sk

Effect of propolis on oxidative stress and histomorphology of liver tissue in experimental obstructive jaundice

Background: Propolis is a natural product collected by honey bees from various plant sources. We aimed to determine the possible effects of propolis on oxidative stress and hepatocyte apoptosis in experimental obstructive jaundice. Methods: Thirty rats were divided into three groups: group I, sham-operated; group II, ligation and division of the common bile duct (BDL); group III, BDL followed by oral supplementation of propolis in a daily dose of 100 mg/kg. Liver samples were examined under the light microscope and transmission electron microscope. Hepatocyte apoptosis was quantitated using the transferase-mediated uridine nick end labeling (TUNEL) assay. Plasma and liver malondialdehyde (MDA) levels and glutathione peroxidase (GSH-Px) activities were measured. Results: The plasma and liver levels of MDA were significantly lower in the propolis group than in the BDL group (p 0.05). In the propolis group, the enlargement of hepatocytes, dilatation of canaliculi and the edema regressed. The regenerating and normal hepatocytes were demonstrated. In the TUNEL assay, propolis administration reduced hepatocyte apoptosis. Conclusion: Propolis showed a significant hepatoprotective effect in this experimental obstructive jaundice model. Copyright (C) 2008 S. Karger AG, Basel