97-101Possible effects of aqueous garlic (Allium
sativum Linn.) and red clover (Trifolium pratense Linn.)
extracts on adenosine deaminase (ADA) activities were investigated in cancerous
and non-cancerous human liver tissues and results were compared with those of
Fludarabine, a known ADA inhibitor. Ten cancerous and 10 non-cancerous adjacent
liver tissues were obtained from patients with metastatic type liver cancer by
surgical operations. Kinetic analyses were carried out to establish Vmax and Km
values of the reaction catalyzed by ADA in the presence or absence of inhibitor.
ADA activity was found to be lower in the cancerous tissues compared with
non-cancerous tissues. Aqueous garlic and red clover extracts induced
significant inhibition ADA activity in both the tissues. Inhibition percentage
was relatively higher in the cancerous tissues as compared with non-cancerous
tissues. Furthermore, we observed that inhibition percentage induced by garlic
and red clover extracts were higher than those of Fludarabine at the
concentrations studied