Glycosylation-related gene expression in prion diseases. PrPSc accumulation in scrapie-infected GT1 cells depends on beta1,4-linked GalNAc-4-SO4 hyposulfation.

absen

Screening Slaughtered Cattle for BSE.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel

Specific determination of the proteinase K-resistant form of the prion protein using two-site immunometric assays. Application to the post-mortem diagnosis of BSE

International audienc

Development of a Bank of Standardized Blood Samples for the Evaluation of Prion Diagnostic Tests

International audienceAn accurate screening of blood donors would constitute a major improvement for transfusion safety from vCJD infection. Several companies already claim positive results with new ante mortem tests for prion in blood but no standardized samples are available to allow comparative evaluations. In the context of the NeuroPrion BloodDiag project, we have constructed a library of blood fraction samples from sheep for the evaluation of these new tests. Sheep were experimentally infected with scrapie and, in parallel with healthy controls, have been sampled regularly since 2004. Blood was fractionated by the French Transfusion Center of Lille (France) using a protocol similar to the one used for humans. With complete information about the animals (genotype, source of contamination, days post-contamination and presence of clinical signs), this library comprises now more than 8000 aliquots from over 110 different blood samples. Assessment of infectivity of the different blood fractions (plasma, platelets, red cells and buffy-coat) has been performed by inoculation of transgenic mice overexpressing ovine PrP gene (tg338). Successful transmissions were observed with the buffy coat of several animals. Now, two years post-infection, some positive results are also being observed with plasma samples inoculated by intraperitoneal route (a still ongoing study). This bank constitutes a powerful tool for the evaluation of blood tests under development for use as pre-clinical diagnostic screens for TSE infection

Heterogeneous phenotype and peripheral prion distribution in a primate model of vCJD blood transmission.

International audienc

Rapid Test for the Preclinical Postmortem Diagnosis of BSE in Central Nervous System Tissue.

Abstract not availableJRC.D-Institute for Reference Materials and Measurements (Geel