9 research outputs found
Genetic variability of verbascum populations from metal polluted and unpolluted sites
Mullein (Verbascum) plants have extensive distribution and can grow in variable environmental conditions. Seed was collected from mullein plants grown at 4 locations, two metals contaminated and two metal uncontaminated areas. Genetic variability of progeny was examined. Populations collected from unpolluted areas were genetically more similar than those collected from polluted areas as revealed by RAPD and SSR markers and UPGMA analysis. The results indicate that there is genetic differentiation between examined populations and therefore they represent suitable material for further investigation of plant adaptation mechanisms to increased metal content
Copper tolerance of trichoderma species
Some Trichoderma strains can persist in ecosystems with high concentrations of heavy metals. The aim of this research was to examine the variability of Trichoderma strains isolated from different ecosystems, based on their morphological properties and restriction analysis of ITS fragments. The fungal growth was tested on potato dextrose agar, amended with Cu(II) concentrations ranging from 0.25 to 10 mmol/l, in order to identify copper-resistant strains. The results indicate that some isolated strains of Trichoderma sp. show tolerance to higher copper concentrations. Further research to examine the ability of copper bioaccumulation by tolerant Trichoderma strains is needed
Species composition of trichoderma communities in Hungarian soils used for vegetable cultivation
Species of the genus Trichoderma are commonly found free-living fungi in soil and rootecosystems.
It is known that the rhizosphere of agricultural soils is an ideal source of
beneficial Trichoderma strains with biocontrol potential, as some of the strains showed
excellent antagonistic abilities against plant pathogenic fungi. Others are able to improve
plant growth, root in particular, promoting drought resistance in some crops.
Biodiversity of Trichoderma isolates from the rizosphere of different vegetables (pepper,
tomato, carrot, salad, spinach, pumpkin, cabbage, kohlrabi, parsley, celery, potato and
bean) in garden soil samples collected at different locations in Hungary (Szeged-Sziksóstó,
Balástya, Hódmezővásárhely, Szentes, Veszprém, Ózd) was comparatively examined
during this study. Trichoderma strains were isolated directly from the chopped roots of the
examined vegetables on dichloran - Rose Bengal medium. DNA isolation and PCR
amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region have been
used for the identification of the isolates and for the investigation of their biodiversity.
Trichoderma isolates were identified based on their ITS sequences with the aid of the
barcoding program TrichOKEY 2.0 available online at the home page of the International
Subcommission on Trichoderma and Hypocrea Taxonomy (www.isth.info).
Among the detected isolates, species known as promising biocontrol agents (T. harzianum,
T. virens, T. atroviride, T. asperellum) could be identified. Data about the biodiversity of
the genus Trichoderma in vegetable rhizosphere and surveying the in vitro antagonistic
abilities of the isolated Trichoderma strains may reveal potential biocontrol agents against
plant pathogenic fungi.
The project is co-financed by the European Union through the Hungary-Serbia IPA Crossborder
Co-operation Programme (PHANETRI, HUSRB/1002/214/068)
Species composition of trichoderma communities in Hungarian soils used for vegetable cultivation
Species of the genus Trichoderma are commonly found free-living fungi in soil and rootecosystems. It is known that the rhizosphere of agricultural soils is an ideal source of beneficial Trichoderma strains with biocontrol potential, as some of the strains showed excellent antagonistic abilities against plant pathogenic fungi. Others are able to improve plant growth, root in particular, promoting drought resistance in some crops. Biodiversity of Trichoderma isolates from the rizosphere of different vegetables (pepper, tomato, carrot, salad, spinach, pumpkin, cabbage, kohlrabi, parsley, celery, potato and bean) in garden soil samples collected at different locations in Hungary (Szeged-Sziksóstó, Balástya, Hódmezővásárhely, Szentes, Veszprém, Ózd) was comparatively examined during this study. Trichoderma strains were isolated directly from the chopped roots of the examined vegetables on dichloran - Rose Bengal medium. DNA isolation and PCR amplification of the internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region have been used for the identification of the isolates and for the investigation of their biodiversity. Trichoderma isolates were identified based on their ITS sequences with the aid of the barcoding program TrichOKEY 2.0 available online at the home page of the International Subcommission on Trichoderma and Hypocrea Taxonomy (www.isth.info). Among the detected isolates, species known as promising biocontrol agents (T. harzianum, T. virens, T. atroviride, T. asperellum) could be identified. Data about the biodiversity of the genus Trichoderma in vegetable rhizosphere and surveying the in vitro antagonistic abilities of the isolated Trichoderma strains may reveal potential biocontrol agents against plant pathogenic fungi
Screening of trichoderma strains isolated from rhizosphere samples for laccase production
In this study we screened formerly isolated Trichoderma strains for laccase production on solid media supplemented with two different substrates, ABTS [2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)] or guaiacol. We detected outstandingly strong colour changes in the case of three Trichoderma strains in this experiment. The strains were identified based on internal transcribed spacer (ITS) sequence analysis as T. asperellum (SZMC 20786 and SZMC 20866) and T. atroviride (SZMC 20780). We also investigated the production of laccase enzymes in the case of these Trichoderma strains in two types of liquid media. The pH dependence of the secreted laccases was determined in cell free ferment broths at pH 3.5, 4, 5, 5.5, 6 and 6.5 adjusted with 25 mM succinate buffer. Laccase activities from liquid cultures were measured with ABTS as substrate. The results showed that the best laccase producer among the investigated Trichoderma strains was T. atroviride SZMC 20780 under these conditions. This strain shows the highest laccase enzyme activity on the second day of incubation in a rotary shaker at 25 °C
Optimization of Liquid Scintillation Spectrometer Quantulus 1220 for tritium determination in water samples after electrolytic enrichment
U radu je prikazana optimizacija uslova merenja na tečnom scintilacionom
spektrometru Ultra Low Level Liquid Scintillation Spectrometer Quantulus 1220 u cilju
određivanja tricijuma u uzorcima voda. Opisan je postupak određivanja efikasnosti,
optimalnog odnosa zapremine uzorka i scintilacionog koktela i optimalno vreme
merenja. Instrument je predviđen za merenje niskih aktivnosti tricijuma u uzorcima
prirodnih voda. U tu svrhu uzorci površinskih voda i padavina su elektrolitički
obogaćeni i kao takvi mereni na spektrometru. Rezultati pokazuju da su dobijene
aktivnosti tricijuma u datim vodama ispod granica predvidjenih zakonskom
regulativom.The paper presents the optimization of the measurement conditions for Ultra Low Level
Liquid Scintillation Spectrometer Quantulus 1220 in order to determine the tritium
activity in water samples after electrolytic enrichment. The procedure included
determination of efficiency, background, minimal detectable activity, the optimal ratio
of the sample volume and scintillation cocktail and the optimal measurement time. The
instrument is designed to measure low activity of tritium in natural water samples. For
this purpose, samples of surface waters and precipitation are electrolytically enriched
and as such are measured on a spectrometer.Зборник радова : XXIX симпозијум ДЗЗСЦГ : Сребрно језеро, 27-29. септембар 2017. годин
Optimization of Liquid Scintillation Spectrometer Quantulus 1220 for tritium determination in water samples after electrolytic enrichment
U radu je prikazana optimizacija uslova merenja na tečnom scintilacionom
spektrometru Ultra Low Level Liquid Scintillation Spectrometer Quantulus 1220 u cilju
određivanja tricijuma u uzorcima voda. Opisan je postupak određivanja efikasnosti,
optimalnog odnosa zapremine uzorka i scintilacionog koktela i optimalno vreme
merenja. Instrument je predviđen za merenje niskih aktivnosti tricijuma u uzorcima
prirodnih voda. U tu svrhu uzorci površinskih voda i padavina su elektrolitički
obogaćeni i kao takvi mereni na spektrometru. Rezultati pokazuju da su dobijene
aktivnosti tricijuma u datim vodama ispod granica predvidjenih zakonskom
regulativom.The paper presents the optimization of the measurement conditions for Ultra Low Level
Liquid Scintillation Spectrometer Quantulus 1220 in order to determine the tritium
activity in water samples after electrolytic enrichment. The procedure included
determination of efficiency, background, minimal detectable activity, the optimal ratio
of the sample volume and scintillation cocktail and the optimal measurement time. The
instrument is designed to measure low activity of tritium in natural water samples. For
this purpose, samples of surface waters and precipitation are electrolytically enriched
and as such are measured on a spectrometer.Зборник радова : XXIX симпозијум ДЗЗСЦГ : Сребрно језеро, 27-29. септембар 2017. годин
Lipodystrophy induced by combination antiretroviral therapy in HIV/AIDS patients: A Belgrade cohort study
Background/Aim. Highly active antiretroviral therapy (HAART) has led to
dramatic reductions in mortality and morbidity of HIV/AIDS-patients.
Lipodystrophy, a syndrome including peripheral fat wasting and central
obesity, is well-documented side effect of HAART. The aim of this study was
to evaluate the incidence of lipodystrophy, and to determine its risk ratios
in a HIV/AIDS cohort. Methods. This cross-sectional study included all
antiretroviral-naive HIV/AIDS patients commencing HAART from October 1, 2001
to October 1, 2010, in the HIV/AIDS Center, Institute of Infectious and
Tropical Diseases, Belgrade, Serbia. Univariate and stepwise multivariate
logistic regression analyses were used to determine the odds ratios (OR) with
the confidence interval (CI) of 95%, in order to establish the relative risk
for lipodystrophy. The Kaplan-Meier-method was used to determine the
probability of development lipodystrophy over time. All statistical analyses
were performed using SPSS software version using 0.05 as a p-treshold for the
significance. Results. This study included 840 HIV/AIDS patients, 608 women
and 232 men, followed for 5.6 ± 2.8 years. The prevalence of lipodystrophy
was 69.2%. Univariate and stepwise multivariate regression analysis
identified that the female gender, hepatitis C coinfection, AIDS diagnosis
prior to HAART initiation, nucleoside-reverse-transcriptase-inhibitors and
proteaseinhibitors based regimens had a high risk for developing
lipodystrophy in HIV/AIDS-patients (OR = 1.6, 95% CI = 1.1-3.49, p = 0.04; OR
= 3.31, 95% CI = 1.4 - 3.8, p < 0.01; OR = 3.7, 95% CI = 1.7 - 6.1, p < 0.01;
OR = 2.1, 95% CI = 1.7 - 3.3, p < 0.01; OR = 6.1, 95% CI = 4.1 - 9.7, p <
0.01, respectively). Conclusion. Despite much greater life expectancy of
HIV/AIDSpatients, treatment-related toxicities still remain a major concern.
Monitoring of lipodystrophy, as side effect of HAART, is particularly
important. [Projekat Ministarstva nauke Republike Srbije, br. 175024 i
“European AIDS Treatment Network” (NEAT): LSHPCT - 2006-037570 within the FP
6 program
Degradation of linuron in soil by two fungal strains
Two fungal strains were applied to soil polluted with herbicide in order to
determine their degradation potential. Three experimental setups were used.
In the first setup, the soil in pots was contaminated by linuron in final
concentration of 1 ppm. Suspensions of Phanerocheate chrysosporium and
Trichoderma asperellum were applied separately or in combination. Tomato
plantlets were transplanted and chlorophyll content in their leaves was
determined at two time points during plant growth. In the second setup in
pots, the final concentration of linuron was lower, 0.45 ppm. In the third
setup 0.1 ppm of linuron was applied in the field plot. Plantlets of lettuce
were transplanted and chlorophyll content was measured as indicator of plant
stress. The content of linuron in soil was determined by HPLC. The applied
fungal strains significantly reduced toxic effect of 0.45 ppm linuron on
plants, which was not the case for 1 ppm linuron. Both fungi, applied
separately or in combination, were effective in decreasing the linuron
content in the soil. However, in field conditions the combination of both
fungi was the most effective. [Projekat Ministarstva nauke Republike Srbije,
br. III43010