The demography of fine roots in response to patches of water and nitrogen

Fine root demography was quantified in response to patches of increased water and nitrogen availability in a natural, second-growth, mixed hardwood forest in northern Michigan, USA. As expected, the addition of water and water plus nitrogen resulted in a significant overall increase in the production of new fine roots. New root production was much greater in response to water plus nitrogen when compared with water alone, and the duration of new root production was related to the length of resource addition in the water plus nitrogen treatments; the average difference in new root length between the 20 vs. 40 d additions of water plus nitrogen amounted to almost 600%. Roots produced in response to the additions of water and water plus nitrogen lived longer than roots in the control treatments. Thus, additions of water and water plus nitrogen influenced both the proliferation of new roots and their longevity, with both proliferation and longevity related to the type and duration of resource supply. Results suggest that root longevity and mortality may be plastic in response to changes in soil resource availability, as is well known for root proliferation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65770/1/j.1469-8137.1993.tb03905.x.pd

Diffuse X-Ray Emission in the Cygnus OB2 Association

We present a large-scale study of diffuse X-ray emission in the nearby massive stellar association Cygnus OB2 as part of the Chandra Cygnus OB2 Legacy Program. We used 40 Chandra X-ray ACIS-I observations covering ∼1.0 deg2. After removing 7924 point sources detected in our survey and applying adaptive smoothing to the background-corrected X-ray emission, the adaptive smoothing reveals large-scale diffuse X-ray emission. Diffuse emission was detected in the subbands soft (0.5−1.2 keV) and medium (1.2−2.5 keV) and marginally in the hard (2.5−7.0 keV) band. From X-ray spectral analysis of stacked spectra we compute a total (0.5-7.0 keV) diffuse X-ray luminosity of L X diff ≈ 4.2 × 1034 erg s−1, characterized by plasma temperature components at kT ≈ 0.11, 0.40, and 1.18 keV, respectively. The H i absorption column density corresponding to these temperatures has a distribution consistent with N H = (0.43, 0.80, 1.39) × 1022 cm−2. The extended medium-band energy emission likely arises from O-type stellar winds thermalized by wind−wind collisions in the most populated regions of the association, while the soft-band emission probably arises from less energetic termination shocks against the surrounding interstellar medium. Supersoft and soft diffuse emission appears more widely dispersed and intense than the medium-band emission. The diffuse X-ray emission is generally spatially coincident with low-extinction regions that we attribute to the ubiquitous influence of powerful stellar winds from massive stars and their interaction with the local interstellar medium. Diffuse X-ray emission is volume filling, rather than edge brightened, oppositely to other star-forming regions. We reveal the first observational evidence of X-ray halos around some evolved massive stars

Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5

Here we report the study of two bioengineered variants of human trace amine-associated receptor 5 (hTAAR5) that were expressed in stable tetracycline-inducible HEK293S cell lines. A systematic detergent screen showed that fos-choline-14 was the optimal detergent to solubilize and subsequently purify the receptors. Milligram quantities of both hTAAR5 variants were purified to near homogeneity using immunoaffinity chromatography followed by gel filtration. Circular dichroism showed that the purified receptors had helical secondary structures, indicating that they were properly folded. The purified receptors are not only suitable for functional analyses, but also for subsequent crystallization trials. To our knowledge, this is the first mammalian TAAR that has been heterologously expressed and purified. Our study will likely stimulate in the development of therapeutic drug targets for TAAR-associated diseases, as well as fabrication of TAAR-based sensing devices