Quantitative expression patterns of genes in leaf tissues of winter Norstar (No), spring Norstar (SN), spring Manitou (Ma), and winter Manitou (WM) during long-term acclimation at 6 °C

(A) , SE of data points=5.3; (B) , SE of data points=2.8; (C) , SE of data points=4.3.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Accumulation of WCS120 polypeptide for selected acclimation duration at 6 °C

(A) Leaf, SE of data points=4.3; (B) crown, SE of data points=1.4. No, winter Norstar; SN, spring Norstar; Ma, spring Manitou; WM, winter Manitou.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Chemiluminescent western blot detection of WCS120 polypeptide acccumulation in crown and leaf tissues during LT exposure (0–70 d)

(A) Winter Norstar. (B) Spring Norstar. (C) Spring Manitou. (D) Winter Manitou. Arrowheads indicate 50 kDa WCS120 polypeptide used for densitometric quantification. L56 is the Norstar leaf sample exposed to LT for 56 d and served as a control for quantification. The asterisk in (C) indicates a 3-fold lower amount of L56 total protein loaded onto the gel. In order to detect the polypeptide in Manitou, a 3-fold higher amount of protein was loaded onto the gel. After densitometric quantification, the values were accordingly adjusted by reducing them 3-fold for Manitou.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Final leaf number (FLN) for Norstar, spring Norstar, Manitou, and winter Manitou acclimated at 6 °C for 98 d

SE of data points=0.32. No, winter Norstar; SN, spring Norstar; Ma, spring Manitou; WM, winter Manitou.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Low temperature acclimation curve for Norstar, spring Norstar, Manitou, and winter Manitou acclimated at 6 °C for 98 d

SE of data points=0.62. No, winter Norstar; SN, spring Norstar; Ma, spring Manitou; WM, winter Manitou.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Quantitative expression patterns of genes in crown tissues of winter Norstar (No), spring Norstar (SN), spring Manitou (Ma), and winter Manitou (WM) during long-term acclimation at 6 °C

(A) , SE of data points=2.4; (B) , SE of data points=4.9; (C) , SE of data points=3.6.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

Supplementary Table S1

List of identified and quantified apoplast proteins collected from the apical meristem and vascular transition zone of winter wheat cv. Norstar crowns. Plants were treated for 0, 21, or 42 d at four degrees Celsius to induce a cold acclimation response

Supplementary Table S2

Apoplastic fluid malate dehydrogenase (MDH) specific activity in ‘Norstar’ shoot apical meristem (SAM) and vascular transition zone (VTZ) following 0, 21, or 42 d of cold acclimation. Means followed by the same letter within each column are not significantly different based on Fisher’s Least Significant Difference test (P < 0.05)