Chemiluminescent western blot detection of WCS120 polypeptide acccumulation in crown and leaf tissues during LT exposure (0–70 d)

Abstract

(A) Winter Norstar. (B) Spring Norstar. (C) Spring Manitou. (D) Winter Manitou. Arrowheads indicate 50 kDa WCS120 polypeptide used for densitometric quantification. L56 is the Norstar leaf sample exposed to LT for 56 d and served as a control for quantification. The asterisk in (C) indicates a 3-fold lower amount of L56 total protein loaded onto the gel. In order to detect the polypeptide in Manitou, a 3-fold higher amount of protein was loaded onto the gel. After densitometric quantification, the values were accordingly adjusted by reducing them 3-fold for Manitou.<p><b>Copyright information:</b></p><p>Taken from "Quantitative expression analysis of selected genes reveals their differential expression in leaf and crown tissues of wheat ( L.) during an extended low temperature acclimation regimen"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2393-2402.</p><p>Published online 26 May 2008</p><p>PMCID:PMC2423658.</p><p></p

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