2 research outputs found

    Unravelling the Links between Phage Adsorption and Successful Infection in Clostridium difficile.

    Get PDF
    Bacteriophage (phage) therapy is a promising alternative to antibiotics for the treatment of bacterial pathogens, including Clostridiumdifficile. However, as for many species, in C. difficile the physical interactions between phages and bacterial cells have not been studied in detail. The initial interaction, known as phage adsorption, is initiated by the reversible attachment of phage tail fibers to bacterial cell surface receptors followed by an irreversible binding step. Therefore binding can dictate which strains are infected by the phage. In this study, we investigated the adsorption rates and irreversible binding of three C. difficile myoviruses: CDHM1, CDHM3 and CDHM6 to ten strains that represent ten prevalent C. difficile ribotypes, regardless of their ability to infect. CDHM1 and CDHM3 phage particles adsorbed by ~75% to some strains that they infected. The infection dynamics for CDHM6 are less clear and ~30% of the phage particles bound to all strains, irrespective of whether a successful infection was established. The data highlighted adsorption is phage-host specific. However, it was consistently observed that irreversible binding had to be above 80% for successful infection, which was also noted for another two C. difficile myoviruses. Furthermore, to understand if there is a relationship between infection, adsorption and phage tail fibers, the putative tail fiber protein sequences of CDHM1, CDHM3 and CDHM6 were compared. The putative tail fiber protein sequence of CDHM1 shares 45% homology at the amino acid level to CDHM3 and CDHM6, which are identical to each other. However, CDHM3 and CDHM6 display differences in adsorption, which highlights that there is no obvious relationship between putative tail fiber sequence and adsorption. The importance of adsorption and binding to successful infection is often overlooked, and this study provides useful insights into host-pathogen interactions within this phage-pathogen system

    Theoretical, numerical, and experimental investigation of pressure rise due to deflagration in confined spaces

    No full text
    Estimating pressure rise due to deflagration in a fully or partially confined space is of practical importance in safety design of a petrochemical plant. Herein, we have developed a new theoretical model to predict the pressure rise due to deflagration in both fully and partially confined spaces. First, the theoretical model was compared and validated against experimental data from the closed-space experiments with hydrogen, methane, propane, and ethane. The theory predicted accurate pressure rises near the stoichiometric regime for all fuel types; outside the stoichiometric regime, especially, for rich mixtures of hydrocarbons with air, the theory over-predicted pressure rise since it does not account for soot formation and the associated energy losses by radiation. Experimental investigation of propane and hydrogen deflagration was conducted in a partially confined space and the theory-based predictions agreed with the data up to 5%. Parametric numerical study was performed to investigate the effect of the initial pressure and temperature of gaseous fuels on pressure rise. (C) 2017 Elsevier Masson SAS. All rights reserved
    corecore