Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp

Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

The acute asymmetric effects of hemiovariectomy on testosterone secretion vary along the estrous cycle. The participation of the cholinergic system

The presence of asymmetry in the capacity of the left and right ovaries to secrete testosterone was analyzed by studying the effects of hemiovariectomy along the estrus cycle one hour after surgery. The effects of ether anesthesia on hormone serum levels were also analyzed. Bilateral ovariectomy and the extirpation of the left ovary performed on the day of proestrus resulted in significantly lower testosterone levels. Compared to the anesthetized group, the effects of perforating the peritoneum unilaterally varied according to the day of the estrous cycle and the side of the peritoneum surgery was performed on. Injecting atropine sulfate (ATR) to control or anesthetized rats on D1 resulted in a significant increase of testosterone serum levels. The effects of perforating the peritoneum on testosterone levels depended on the cholinergic innervation and varied along the estrous cycle. Blocking the cholinergic system before performing unilateral or bilateral ovariectomy had different effects depending on the day of the estrous cycle. Testosterone plasma levels increased significantly when surgery was performed on the day of diestrus and dropped when surgery was performed on proestrus. Similar effects were observed when the left adrenal was extirpated from animals with the cholinergic system blocked. The results presented herein support the hypothesis of asymmetry in the ovaries' abilities to secrete steroid hormones, and that the capacity to secrete testosterone varies along the estrous cycle

A new pathology in the red abalone Haliotis rufescens (Mollusca: Gastropoda) cultured in Baja California, Mexico, associated with macro-crystal inclusions in gonadal tissue

A new severe pathological alteration in the gonadic tissue of red abalone was observed, which compromises reproduction of affected organisms. The alteration is related to the presence of numerous extracellularmacro-crystal inclusions (Mci) reaching up to 300 ?m in size. These Mci may be surrounded by hemocytes, cellular debris, and deposition of fibroblasts. Moreover, hypertrophy of the nuclei of cells in the topgerminal epithelium and presence of brown cells in trabeculae were observed. The normal architecture of the tissue looks entirely altered and destroyed in some cases. No gonadal development was observed.The chemical nature of the Mci is unknown, but it is possibly related to inorganic toxic compounds. No previous records of similar alterations in abalone are known to exist. This record could help determine if this pathology has been observed in other abalone culture areas in the world, its possible origin, and if control is necessary

Acute effects of unilateral sectioning the superior ovarian nerve of rats with unilateral ovariectomy on ovarian hormones (progesterone, testosterone and estradiol) levels vary during the estrous cycle

The present study analyzed the participation of the left and right superior ovarian nerves (SON) in regulating progesterone, testosterone, and estradiol serum levels in unilaterally ovariectomized rats on each day of the estrous cycle. For this purpose, ovarian hormone concentrations in serum were measured in animals with either sham-surgery, unilateral ovariectomy (ULO), unilateral sectioning of the SON, or sectioning of the SON innervation of the in situ ovary in rats with ULO

Propuesta de actualización del programa de estudio de la asignatura Contabilidad Gubernamental: plan de estudio 1994 de la carrera Licenciatura en Contaduría Pública de la Universidad de El Salvador

Esta investigación tiene por objetivo conocer y explicar las necesidades de formación profesional en el área de Contabilidad Gubernamental que requiere el Contador Público para un ejercicio eficiente en su desempeño profesional. En base al problema planteado y a los objetivos que se persiguen con la investigación, se establecerán el método y el tipo de investigación así como las estrategias a seguir para la recolección de información, en este caso se trata de una investigación cualitativa y se utilizará el método inductivo deductivo. Se concluye: el desarrollo del Sector Público y la implementación de nuevos modelos económicos nos confirman que el Programa de Estudios de la asignatura Contabilidad Gubernamental se encuentra desactualizado. Con la actualización del Programa de Estudio de la asignatura Contabilidad Gubernamental se contribuirá a mejorar la enseñanza-aprendizaje en dicha materia. El docente encargado de impartir la cátedra presenta deficiencias en cuanto a pedagogía y didáctica se refiere, por lo que se hace necesario capacitarlo en dicha áre

Effect of consecutive matches on heart rate variability in elite volleyball players

El objetivo del estudio fue realizar observar y determinar los cambios en la variabilidad de la frecuencia cardiaca (VFC) de un equipo de voleibol masculino durante su participación en la liga profesional nacional de México. Se registró a 12 atletas (Edad 22.60 ± 3.4) por cuatro meses durante el fin de semana (sábado y domingo). En cada jornada se realizaron tres mediciones, la primera por la mañana en reposo (REST); la segunda por la tarde al finalizar los partidos del primer día (R2) y la tercera al finalizar los partido del segundo día (R4). Los datos se analizaron con el test ANOVA y el test de Bonferroni. Se encontró diferencia entre REST con respecto a la R2 y con la R4 en SDNN (98.66±62.50 ms vs. 50.35±26.33, p < .001; 59.24±22.11, p < .001 para R2 y R4 respectivamente), en la rMSSD (43.49±34.60 ms vs. 24.33±18.89, p < .05; 28.44±16.70, p < .05 para R2 y R4 respectivamente), en el pNN50 (30.37±21.47 % vs. 12.09±16.56, p < .001; 13.30±13.64, p < .001 para R2 y R4 respectivamente), en SD1 (45.84±30.17 ms vs. 22.83±17.30, p < .001; 28.01±14.94, p < .01 para R2 y R4 respectivamente) y en SD2 (123.18±53.67 ms vs. 65.58±32.42, p < .001; 79.06±28.33, p < .001 para R2 y R4 respectivamente), entre la R2 y la R4 no hay diferencias significativas. Los resultados indican que en jugadores de voleibol posterior a varios partidos permite determinar los cambios en la modulación simpática y parasimpática a través de la VFC brindando información sobre el estado de rendimiento físico de los atletas.The objective of this study was to observe and determine changes in HRV parameters in volleyball players during the Mexican national professional volleyball league. Heart rate was monitored in 12 athletes (age 22.60 ± 3.4) for four months during weekends (Saturdays and Sundays). Three measures were taken during each match-day: the first at rest (REST), the second in the afternoon after the matches of the first day (R2), and the third after the matches of the second day (R4). Data were analyzed with ANOVA and the Bonferroni method. We found a difference between REST and R2 and R4 in SDNN (98.66 ± 62.50 vs. 50.35 ± 26.33 ms, p < .001; 59.24 ± 22.11, p < .001 for R2 and R4 respectively), rMSSD (43.49 ± 34.60 vs. 24.33 ± 18.89, p < .05; 28.44 ± 16.70, p < .05 for R2 and R4, respectively), pNN50 (30.37 ± 21.47% vs. 12.09 ± 16.56, p < .001; 13.30 ± 13.64, p < .001 for R2 and R4 respectively), SD1 (45.84 ± 30.17 ms vs. 22.83 ± 17.30, p < .001; 28.01 ± 14.94, p <.01 for R2 and R4, respectively) and SD2 (123.18 ± 53.67 ms vs. 65.58 ± 32.42, p < .001; 79.06 ± 28.33, p < .001 for R2 and R4, respectively). There were no significant differences between R2 and R4. These results show that heart rate variability helps determine changes after several matches in sympathetic and parasympathetic modulation in volleyball players, and that this provides information on athletes' physical performance.O objetivo do estudo foi realizar, observar e determinar mudanças na variabilidade da frequência cardíaca (VFC) da equipe de voleibol dos homens, enquanto participavam na nacional liga profissional México. Foram registrados 12 atletas (entre 22,60 ± 3,4 anos de idade) por quatro meses durante o fim de semana (sábado e domingo). Cada dia se realizaram três medições, a primeira de manhã em repouso (REST); a segunda pela tarde no final das primeiros jogos do dia (R2) e a terceira na parte final das jogos do segundo dia (R4). Os dados foram analisados com o teste ANOVA e teste de Bonferroni. Uma diferença foi encontrada entre o REST no que diz respeito a R2 e R4 em SDNN (98,66 ± 62,50 ms vs 50,35 ± 26,33 ms, p < 0,001; 59,24 ± 22,11, p < 0,001 para R2 e R4, respectivamente), em rMSSD (43,49 ± 34,60 ms vs 24,33 ± 18,89, p < 0,05; 28,44 ± 16,70, p < 0,05, para R2 e R4, respectivamente), no pNN50 (30,37 ± 21,47% vs 12,09 ± 16,56%, p < 0,001; 13,30 ± 13,64, p < 0,001 para R2 e R4, respectivamente), SD1 (45,84 ± 30.17 ms vs. 22,83 ± 17,30, p < 0,001; 28,01 ± 14,94, p < 0,01 para R2 e R4, respectivamente) e no SD2 (123,18 ± 53,67 ms vs 65,58 ± 32,42 ms, p < 0,001; 79,06 ± 28,33, p < 0,001 para R2 e R4, respectivamente), entre R2 e R4 não há diferenças significativas. Os resultados indicam que, nos jogadores de vôlei trás várias jogos, permite determinar alterações na modulação simpático e parassimpático através do VFC fornecendo informações sobre o estado de desempenho físico dos atletas

Efecto del anión del hidróxido doble laminar ZnAl en la intercalación con estreptomicina para la inhibición de Escherichia coli

En este trabajo se estudió la preparación material híbrido de carácter orgánico/inorgánico, a base de estreptomicina con hidróxidos dobles laminares (HDL), la intercalación de la estreptomicina dentro de los HDL se llevó a cabo por intercambio iónico, estudiando la influencia del anión original en el HDL como son NO₃ y Cl-. La caracterización de los materiales se llevó a cabo por las siguientes técnicas, difracción de rayos X, espectroscopia de IR por transformada de Fourier- ATR. La evaluación de la actividad antibacterial de estos materiales se llevó a cabo en cultivos de cepa de Escherichia coli ATCC® 25922 aplicando la técnica de difusión en agar para la determinación de positiva de los materiales, por último, se realizó la cinética de inhibición bacteriana en medio líquido a diferentes concentraciones del material hibrido para determinar la concentración mínima inhibitoria.In this work, we studied the preparation of hybrid material organic/inorganic in nature, based in streptomycin using layered double hydroxide (LDH), the intercalation of streptomycin into the HDL was carried with ionic exchange, studying the influence of the original anion within LDH which are NO₃ and Cl-. The characterization of the material was studied in X-ray diffraction, IR-FT-ATR. The evaluation of the antibacterial activity was done with Escherichia coli strain ATCC 25922, applying the material in the agar, also known as agar diffusion to test and determinate if the hybrid materials became positive to it. For last a kinetic were carried in liquid media using different concentration of hybrid material to determinate the minimal inhibitory concentration