734 research outputs found
Dihydropteroate synthase gene mutations in Pneumocystis and sulfa resistance
Pneumocystis pneumonia (PCP) remains a major cause of illness and death in HIV-infected persons. Sulfa drugs, trimethoprim-sulfamethoxazole (TMP-SMX) and dapsone are mainstays of PCP treatment and prophylaxis. While prophylaxis has reduced the incidence of PCP, its use has raised concerns about development of resistant organisms. The inability to culture human Pneumocystis, Pneumocystis jirovecii, in a standardized culture system prevents routine susceptibility testing and detection of drug resistance. In other microorganisms, sulfa drug resistance has resulted from specific point mutations in the dihydropteroate synthase (DHPS) gene. Similar mutations have been observed in P. jirovecii. Studies have consistently demonstrated a significant association between the use of sulfa drugs for PCP prophylaxis and DHPS gene mutations. Whether these mutations confer resistance to TMP-SMX or dapsone plus trimethoprim for PCP treatment remains unclear. We review studies of DHPS mutations in P. jirovecii and summarize the evidence for resistance to sulfamethoxazole and dapsone
Use of Chemical Modification To Elucidate RNA Folding Pathways
As discussed in this overview, chemical modification is sensitive to the accessibility of a nucleotide to the solvent, and many nucleotides become less accessible as an RNA folds into its structured form. Chemical modification reagents are therefore suitable for following RNA folding, and can be used to study the kinetics of structure formation on time scales ranging from minutes to hours.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143596/1/cpnc1109.pd
Adiabatic following criterion, estimation of the nonadiabatic excitation fraction and quantum jumps
An accurate theory describing adiabatic following of the dark, nonabsorbing
state in the three-level system is developed. An analytical solution for the
wave function of the particle experiencing Raman excitation is found as an
expansion in terms of the time varying nonadiabatic perturbation parameter. The
solution can be presented as a sum of adiabatic and nonadiabatic parts. Both
are estimated quantitatively. It is shown that the limiting value to which the
amplitude of the nonadiabatic part tends is equal to the Fourier component of
the nonadiabatic perturbation parameter taken at the Rabi frequency of the
Raman excitation. The time scale of the variation of both parts is found. While
the adiabatic part of the solution varies slowly and follows the change of the
nonadiabatic perturbation parameter, the nonadiabatic part appears almost
instantly, revealing a jumpwise transition between the dark and bright states.
This jump happens when the nonadiabatic perturbation parameter takes its
maximum value.Comment: 33 pages, 8 figures, submitted to PRA on 28 Oct. 200
Statistical Mechanics for Unstable States in Gel'fand Triplets and Investigations of Parabolic Potential Barriers
Free energies and other thermodynamical quantities are investigated in
canonical and grand canonical ensembles of statistical mechanics involving
unstable states which are described by the generalized eigenstates with complex
energy eigenvalues in the conjugate space of Gel'fand triplet. The theory is
applied to the systems containing parabolic potential barriers (PPB's). The
entropy and energy productions from PPB systems are studied. An equilibrium for
a chemical process described by reactions is also
discussed.Comment: 14 pages, AmS-LaTeX, no figur
Thermally activated escape rates of uniaxial spin systems with transverse field
Classical escape rates of uniaxial spin systems are characterized by a
prefactor differing from and much smaller than that of the particle problem,
since the maximum of the spin energy is attained everywhere on the line of
constant latitude: theta=const, 0 =< phi =< 2*pi. If a transverse field is
applied, a saddle point of the energy is formed, and high, moderate, and low
damping regimes (similar to those for particles) appear. Here we present the
first analytical and numerical study of crossovers between the uniaxial and
other regimes for spin systems. It is shown that there is one HD-Uniaxial
crossover, whereas at low damping the uniaxial and LD regimes are separated by
two crossovers.Comment: 4 PR pages, 3 figures, final published versio
Field dependence of the temperature at the peak of the ZFC magnetization
The effect of an applied magnetic field on the temperature at the maximum of
the ZFC magnetization, , is studied using the recently obtained
analytic results of Coffey et al. (Phys. Rev. Lett. {\bf 80}(1998) 5655) for
the prefactor of the N\'{e}el relaxation time which allow one to precisely
calculate the prefactor in the N\'{e}el-Brown model and thus the blocking
temperature as a function of the coefficients of the Taylor series expansion of
the magnetocrystalline anisotropy. The present calculations indicate that even
a precise determination of the prefactor in the N\'{e}el-Brown theory, which
always predicts a monotonic decrease of the relaxation time with increasing
field, is insufficient to explain the effect of an applied magnetic field on
the temperature at the maximum of the ZFC magnetization. On the other hand, we
find that the non linear field-dependence of the magnetization along with the
magnetocrystalline anisotropy appears to be of crucial importance to the
existence of this maximum.Comment: 14 LaTex209 pages, 6 EPS figures. To appear in J. Phys.: Condensed
Matte
Can the Existence of Dark Energy Be Directly Detected?
The majority of astronomers and physicists accept the reality of dark energy
and also believe that it can only be studied indirectly through observation of
the motions of stars and galaxies. In this paper I open the experimental
question of whether it is possible to directly detect dark energy through the
presence of dark energy density. Two thirds of this paper outlines the major
aspects of dark energy density as now comprehended by the astronomical and
physics community. The final third summarizes various proposals for direct
detection of dark energy density or its possible effects. At this time I do not
have a fruitful answer to the question: Can the Existence of Dark Energy Be
Directly Detected?Comment: 4 page
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