Barbara Johnston of Sarnia, Ontario: The First Canadian Pentecostal Missionary to India

In Canada, three early geographic centers of Pentecostal revival emerged within the first decade of the twentieth century, along with its leaders: R. E. McAlister in Ottawa, Ellen Hebden in Toronto, and A. H. Argue and his family in Winnipeg. Ellen Hebden is the first person known to have received Pentecostal baptism in Canada.  We know some detail about these personalities and their work in these cities largely because of the earliest Pentecostal newsletters that have survived until today: The Promise, published in Toronto between 1907 and 1910 by Ellen and James Hebden, The Good Report, published in Ottawa between 1911 and 1912 by Herbert Randall, H. L. Lawler, and R. E. McAlister, and The Apostolic Messenger, published in Winnipeg between 1908 and 1910 by A. H. Argue.  These Canadian newsletters, along with some international newsletters, reported information about the experiences of connected missionaries and evangelistic efforts. Several small Canadian towns and cities are mentioned by name in these newsletters.  The acknowledgement of these little-known communities hints at the establishment of Pentecostalism in less urban centres, each locus having its own story and distinct personalities. As yet, most of these stories remain untold, lost, or forgotten. This biographical portrait of Barbara Johnston of Sarnia, Ontario, is an attempt to reclaim one such story, with a hope that readers might be inspired to recover and retell similar accounts of other early Canadian Pentecostals

Pentecostal Identity, Beliefs, and Praxis: A Review Essay

Review by Caleb Courtney, Independent Scholar, Sarnia, ON

The TESS-Keck Survey. XI. Mass Measurements for Four Transiting sub-Neptunes orbiting K dwarf TOI-1246

Multi-planet systems are valuable arenas for investigating exoplanet architectures and comparing planetary siblings. TOI-1246 is one such system, with a moderately bright K dwarf (V=11.6, K=9.9) and four transiting sub-Neptunes identified by TESS with orbital periods of 4.31 d, 5.90 d, 18.66 d, and 37.92 d. We collected 130 radial velocity observations with Keck/HIRES and TNG/HARPS-N to measure planet masses. We refit the 14 sectors of TESS photometry to refine planet radii (2.97±0.06 R⊕,2.47±0.08 R⊕,3.46±0.09 R⊕, 3.72±0.16 R⊕), and confirm the four planets. We find that TOI-1246 e is substantially more massive than the three inner planets (8.1±1.1M⊕, 8.8±1.2M⊕, 5.3±1.7M⊕, 14.8±2.3M⊕). The two outer planets, TOI-1246 d and TOI-1246 e, lie near to the 2:1 resonance (Pe/Pd=2.03) and exhibit transit timing variations. TOI-1246 is one of the brightest four-planet systems, making it amenable for continued observations. It is one of only six systems with measured masses and radii for all four transiting planets. The planet densities range from 0.70±0.24 to 3.21±0.44g/cm3, implying a range of bulk and atmospheric compositions. We also report a fifth planet candidate found in the RV data with a minimum mass of 25.6 ± 3.6 M⊕. This planet candidate is exterior to TOI-1246 e with a candidate period of 93.8 d, and we discuss the implications if it is confirmed to be planetary in nature

The TESS-Keck Survey. XI. Mass Measurements for Four Transiting Sub-Neptunes Orbiting K Dwarf TOI-1246

Multiplanet systems are valuable arenas for investigating exoplanet architectures and comparing planetary siblings. TOI-1246 is one such system, with a moderately bright K dwarf (V = 11.6, K = 9.9) and four transiting sub-Neptunes identified by TESS with orbital periods of 4.31, 5.90, 18.66, and 37.92 days. We collected 130 radial velocity observations with Keck/HIRES and TNG/HARPS-N to measure planet masses. We refit the 14 sectors of TESS photometry to refine planet radii (2.97 +/- 0.06 R (circle plus), 2.47 +/- 0.08 R (circle plus), 3.46 +/- 0.09 R (circle plus), and 3.72 +/- 0.16 R (circle plus)) and confirm the four planets. We find that TOI-1246 e is substantially more massive than the three inner planets (8.1 +/- 1.1 M (circle plus), 8.8 +/- 1.2 M (circle plus), 5.3 +/- 1.7 M (circle plus), and 14.8 +/- 2.3 M (circle plus)). The two outer planets, TOI-1246 d and TOI-1246 e, lie near to the 2:1 resonance (P (e)/P ( d ) = 2.03) and exhibit transit-timing variations. TOI-1246 is one of the brightest four-planet systems, making it amenable for continued observations. It is one of only five systems with measured masses and radii for all four transiting planets. The planet densities range from 0.70 +/- 0.24 to 3.21 +/- 0.44 g cm(-3), implying a range of bulk and atmospheric compositions. We also report a fifth planet candidate found in the RV data with a minimum mass of 25.6 +/- 3.6 M (circle plus). This planet candidate is exterior to TOI-1246 e, with a candidate period of 93.8 days, and we discuss the implications if it is confirmed to be planetary in nature

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

CSF1R inhibitor JNJ-40346527 attenuates microglial proliferation and neurodegeneration in P301S mice

Neuroinflammation and microglial activation are significant processes in Alzheimer’s disease pathology. Recent genome-wide association studies have highlighted multiple immune-related genes in association with Alzheimer’s disease, and experimental data have demonstrated microglial proliferation as a significant component of the neuropathology. In this study, we tested the efficacy of the selective CSF1R inhibitor JNJ-40346527 (JNJ-527) in the P301S mouse tauopathy model. We first demonstrated the anti-proliferative effects of JNJ-527 on microglia in the ME7 prion model, and its impact on the inflammatory profile, and provided potential CNS biomarkers for clinical investigation with the compound, including pharmacokinetic/pharmacodynamics and efficacy assessment by TSPO autoradiography and CSF proteomics. Then, we showed for the first time that blockade of microglial proliferation and modification of microglial phenotype leads to an attenuation of tau-induced neurodegeneration and results in functional improvement in P301S mice. Overall, this work strongly supports the potential for inhibition of CSF1R as a target for the treatment of Alzheimer’s disease and other tau-mediated neurodegenerative diseases

Characterization of Osteoblastic Properties of 7F2 and UMR-106 Cultures after Acclimation to Reduced Levels of Fetal Bovine Serum

Estrogen plays an important role in skeletal physiology by maintaining a remodeling balance between the activity of osteoblasts and osteoclasts. In an attempt to decipher the mechanism through which estrogen elicits its action on osteoblasts, experimentation necessitated the development of a culturing environment reduced in estrogenic compounds. The selected media (OPTI-MEM) is enriched to sustain cultures under reduced fetal bovine serum (FBS) conditions and is devoid of the pH indicator phenol red. This protocol reduced the concentration of FBS supplementation to 0% through successive, 24-hour incubations with diminishing amounts of total FBS (1%, 0.1%, and 0%) and incorporated the use of charcoal filtered FBS. Although the rate of mitotic divisions declined, the protocol does not appear to alter the viability, cell morphology or osteoblast-like phenotype of 7F2 and UMR-106 cultures when compared to control cells grown in various concentrations of FBS. Utilizing a variety of techniques, cultures exposed to media devoid of FBS maintained the ability to express numerous osteoblast specific markers and some markers exhibited β-estradiol responsiveness. Hence, the cell culture protocol developed allowed cultures to proliferate while maintaining their osteoblastic phenotype and provides an alternative avenue to study the anti-resorptive role of estrogen on skeletal turnover. The use of calcium channel antagonists (diltiazem and nifedipine), to block voltage-regulated L-type calcium channels, were also investigated. This avenue of study was initially designed to observe the viability of osteoblast-like cells exposed to the antagonists and ultimately the flow of calcium across their membranes in a significantly reduced estrogen environment

The TESS-Keck Survey. XVIII. A Sub-Neptune and Spurious Long-period Signal in the TOI-1751 System

We present and confirm TOI-1751 b, a transiting sub-Neptune orbiting a slightly evolved, solar-type, metal-poor star (T eff = 5996 ± 110 K, log ( g ) = 4.2 ± 0.1 , V = 9.3 mag, [Fe/H] = −0.40 ± 0.06 dex) every 37.47 days. We use TESS photometry to measure a planet radius of 2.77 − 0.07 + 0.15 R ⊕ . We also use both Keck/HIRES and APF/Levy radial velocities (RV) to derive a planet mass of 14.5 − 3.14 + 3.15 M ⊕ , and thus a planet density of 3.6 ± 0.9 g cm−3. There is also a long-period (∼400 days) signal that is observed in only the Keck/HIRES data. We conclude that this long-period signal is not planetary in nature and is likely due to the window function of the Keck/HIRES observations. This highlights the role of complementary observations from multiple observatories to identify and exclude aliases in RV data. Finally, we investigate the potential compositions of this planet, including rocky and water-rich solutions, as well as theoretical irradiated ocean models. TOI-1751 b is a warm sub-Neptune with an equilibrium temperature of ∼820 K. As TOI-1751 is a metal-poor star, TOI-1751 b may have formed in a water-enriched formation environment. We thus favor a volatile-rich interior composition for this planet.</p

The KELT Follow-up Network and Transit False-positive Catalog: Pre-vetted False Positives for TESS

The Kilodegree Extremely Little Telescope (KELT) project has been conducting a photometric survey for transiting planets orbiting bright stars for over ten years. The KELT images have a pixel scale of ~23"/pixel---very similar to that of NASA's Transiting Exoplanet Survey Satellite (TESS)---as well as a large point spread function, and the KELT reduction pipeline uses a weighted photometric aperture with radius 3'. At this angular scale, multiple stars are typically blended in the photometric apertures. In order to identify false positives and confirm transiting exoplanets, we have assembled a follow-up network (KELT-FUN) to conduct imaging with higher spatial resolution, cadence, and photometric precision than the KELT telescopes, as well as spectroscopic observations of the candidate host stars. The KELT-FUN team has followed-up over 1,600 planet candidates since 2011, resulting in more than 20 planet discoveries. Excluding ~450 false alarms of non-astrophysical origin (i.e., instrumental noise or systematics), we present an all-sky catalog of the 1,128 bright stars (6<V<10) that show transit-like features in the KELT light curves, but which were subsequently determined to be astrophysical false positives (FPs) after photometric and/or spectroscopic follow-up observations. The KELT-FUN team continues to pursue KELT and other planet candidates and will eventually follow up certain classes of TESS candidates. The KELT FP catalog will help minimize the duplication of follow-up observations by current and future transit surveys such as TESS.Comment: Accepted for publication in AJ, 21 pages, 12 figures, 7 table