Multi-omic approach to evaluate the response of gilt-head sea bream (Sparus aurata) exposed to the UV filter sulisobenzone

Sulisobenzone (BP-4) is one of the benzophenone type UVfiltersmost frequently detected in aquatic ecosystems. As a suspected endocrine disrupting compound, scarce information is available yet about other molecular effects and its mechanismof action. Here, we used an integrated transcriptomic and metabolomic approach to improve the current understanding on the toxicity of BP-4 towards aquatic species. Gilt-head sea bream individualswere exposed at environmentally relevant concentrations (10 μg L-1) for 22 days. Transcriptomic analysis revealed 371 differentially expressed genes in liver while metabolomic analysis identified 123 differentially modulated features in plasma and 118 in liver. Integration of transcriptomic and metabolomic data showed disruption of the energy metabolism(>10 pathways related to the metabolismof amino acids and carbohydrateswere impacted) and lipid metabolism (5 glycerophospholipids and the expression of 3 enzymes were affected), suggesting oxidative stress.We also observed, for the first time in vivo and at environmental relevant concentrations, the disruption of several enzymes involved in the steroid and thyroid hormones biosynthesis. DNA and RNA synthesis was also impacted by changes in the purine and pyrimidine metabolisms. Overall, the multiomic workflow presented here increases the evidence on suspected effects of BP-4 exposure and identifies additional modes of action of the compounds that could have been overlooked by using single omic approaches. ©2021 The Authors. Published by Elsevier B.V.This research was supported by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) (CTM2015-70731-R) and the FPI fellowship (BES-2016-078593). The authors also would like to acknowledge the Laboratory of Aquaculture at the Faculty of Marine and Environmental Sciences (University of Cádiz), Thibaut Dumas and David Rosain (UMR HydroSciences, Université Montpellier, France) for their support

Ville et district dans la région centre aux xixe et xxe siècles : l’exemple de la chemiserie d’Argenton-Sur-Creuse

L’industrie de la confection s’est implantée au milieu du xixe siècle dans le département de l’Indre. Elle a rapidement occupé une bonne partie de la population féminine du département et a envahi les villes, les faubourgs et les campagnes. Dès 1848, une activité de confection d’articles en grandes séries est signalée autour de Chabris, près de Valençay. À Argenton, ville de 6 320 habitants en 1886 et chef-lieu de canton, le premier atelier fonctionne en 1860. Dans les années 1930, les syndic..

What role can play omics as a tool for risk assessment and environmental monitoring?

International audienceChoix des outils d'analyse le détail du design expéimental. Présentation des premiers résultats d'accumulation, de recherche de métabolite ainsi que le traitement des premiers résultats d'effet moléculaire, notamment avec le mécanisme d'action de la fluoxétine connu chez l'homme

Caractérisation de l'exposition de l'enfant prépubère aux hormones stéroïdes gonadiques

Une problématique a été soulevée ces dernières années quant aux effets néfastes potentiels pouvant résulter de l'exposition à des substances chimiques ayant la capacité d'interférer avec le système endocrinien. Notre projet consistait à étudier dans quelle mesure l'ingestion d hormones stéroïdes via l alimentation peut représenter un risque pour l enfant prépubère. Dans un premier temps, une nouvelle stratégie analytique pour la mesure d hormones stéroïdes à l état d ultra-traces (ng.L-1) dans les aliments et dans des matrices biologiques complexes tel que le sérum a été développée. Dans un second temps, l'application de la méthode développée à des aliments (lait, œuf, viande) a permis de caractériser l apport alimentaire en hormones stéroïdes de cette population. Enfin, la quantification des androgènes et estrogènes dans plus de 120 échantillons de sérum d enfants âgés de 6 à 16 ans a donné accès aux concentrations plasmatiques circulantes en hormones stéroïdes, et donc à la production endogène de cette population. Finalement, une première interprétation de ces données, dans un cadre général d appréciation du risque lié à ces composés est présentée, en lien avec les différentes recommandations du Codex Alimentarius et de la FDA.Global concerns have been raised in recent years over the potential adverse effects that may result from exposure to chemicals that have the capacity to interfere with the endocrine system. Our main purpose was to investigate in what extent food intake of steroid hormones can represent a risk for prepubertal children. At first, a new analytical strategy for the measurement of steroid hormones at ultra-trace level in food and complex biological matrices was developed. Subsequently, steroid measurements in various food products (milk, egg and meat) were performed in order to evaluate the food intake in steroid hormones for this population. Then, the quantification of the main androgens and estrogens in more than 120 serum samples of children aged from 6 to 16 years gave access to the steroid plasmatic hormonal rates and consequently to the daily endogenous production of this population. A first interpretation of these data in term of risk assessment is also provided, in connection with existing CODEX, JECFA and FDA recommendations regarding maximal acceptable daily intake for estradiol.NANTES-BU Sciences (441092104) / SudocSudocFranceF

Exposition de moules méditerranéennes à un anti-dépresseur : recherche des métabolites de la fluoxétine formés suite à l’exposition.

International audienceCe poster présente le développement d'une méthode d'analyse de la fluoxétine, un antidépresseur, dans les tissus de la moule méditerranéenne (Mytilus galloprovincialis), ainsi qu'une stratégie novatrice pour la recherche des métabolites de la fluoxétine par une analyse non-ciblée

Environmental Metabolomics Promises and Achievements in the Field of Aquatic Ecotoxicology: Viewed through the Pharmaceutical Lens

International audienceScientists often set ambitious targets using environmental metabolomics to address challenging ecotoxicological issues. This promising approach has a high potential to elucidate the mechanisms of action (MeOAs) of contaminants (in hazard assessments) and to develop biomarkers (in environmental biomonitoring). However, metabolomics fingerprints often involve a complex mixture of molecular effects that are hard to link to a specific MeOA (if detected in the analytical conditions used). Given these promises and limitations, here we propose an updated review on the achievements of this approach. Metabolomics-based studies conducted on the effects of pharmaceutical active compounds in aquatic organisms provide a relevant means to review the achievements of this approach, as prior knowledge about the MeOA of these molecules could help overcome some shortcomings. This review highlighted that current metabolomics advances have enabled more accurate MeOA assessment, especially when combined with other omics approaches. The combination of metabolomics with other measured biological endpoints has also turned out to be an efficient way to link molecular effects to (sub)-individual adverse outcomes, thereby paving the way to the construction of adverse outcome pathways (AOPs). Here, we also discuss the importance of determining MeOA as a key strategy in the identification of MeOA-specific biomarkers for biomonitoring. We have put forward some recommendations to take full advantage of environmental metabolomics and thus help fulfil these promises

Non-targeted approaches to assess environmental pollution

International audienc

Occurrence et bioaccumulation des filtres UV chez Mytilus galloprovincialis

International audienc

Eco-exposome aquatique : les questions et leur intégration, un défi à relever

International audienc

Urinary excretion of sex steroid hormone metabolites after consumption of cow milk: a randomized crossover intervention trial

International audienc