The Making of a Boxer

Inspired by the resounding success of Abina and the Important Men (OUP, 2011), Mendoza the Jew combines a graphic history with primary documentation and contextual information to explore issues of nationalism, identity, culture, and historical methodology through the life story of Daniel Mendoza. Mendoza was a poor Sephardic Jew from East London who became the boxing champion of Britain in 1789. As a Jew with limited means and a foreign-sounding name, Mendoza was an unlikely symbol of what many Britons considered to be their very own national sport. Whereas their adversaries across the Channel reputedly settled private quarrels by dueling with swords or pistols--leaving widows and orphans in their wake--the British (according to supporters of boxing) tended to settle their disputes with their fists.Mendoza the Jew provides an exciting and lively alternative to conventional lessons on nationalism. Rather than studying learned treatises and political speeches, students can read a graphic history about an eighteenth-century British boxer that demonstrates how ideas and emotions regarding the nation permeated the practices of everyday life. Mendoza\u27s story reveals the ambivalent attitudes of British society toward its minorities, who were allowed (sometimes grudgingly) to participate in national life by braving pain and injury in athletic contests, but whose social mobility was limited and precarious.https://scholarworks.wm.edu/asbookchapters/1143/thumbnail.jp

General and specific interactions of the phospholipid bilayer with P-type ATPases

Protein structure and function are modulated via interactions with their environment, representing both the surrounding aqueous media and lipid membranes that have an active role in shaping the structural topology of membrane proteins. Compared to a decade ago, there is now an abundance of crystal structural data on membrane proteins, which together with their functional studies have enhanced our understanding of the salient features of lipid-protein interactions. It is now important to recognize that membrane proteins are regulated by both: (1) general lipid-protein interactions, where the general physicochemical properties of the lipid environment affect the conformational flexibility of a membrane protein; and (2) by specific lipid-protein interactions, where lipid molecules directly interact via chemical interactions with specific lipid-binding sites located on the protein. However, due to local differences in membrane composition, thickness and lipid packing, local membrane physical properties and hence the associated lipid-protein interactions also differ due to membrane location, even for the same protein. Such a phenomenon has been shown to be true for one family of integral membrane ion pumps, the P2-type adenosine triphosphatases (ATPases). Despite being highly homologous, individual members of this family have distinct structural and functional activity and are an excellent candidate to highlight how the local membrane physical properties and specific lipid-protein interactions play a vital role in facilitating the structural rearrangements of these proteins necessary for their activity. Hence in this review, we focus on both the general and specific lipid-protein interactions and will mostly discuss the structure-function relationships of the following P2-type ATPases, Na+,K+-ATPase (NKA), gastric H+,K+-ATPase (HKA) and sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA), in concurrence with their lipid environment.Australian Research Counci

Thermal-neutron cross section for 10(n,t)2_ via 3-4He mass spectrometry

The thermal cross section for the reaction 10He is determined to be 4.47±0.15 mb by neutron irradiation of H3BO3 followed by measurement of 3He (from decay of 3H) and 4He in a static mass spectrometer. Some samples contained boron of normal isotopic composition and some were enriched in 10B, and irradiations were carried out in a highly thermalized neutron flux and in the core of a light-water-moderated reactorPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/86120/1/PhysRevC.39.1633.pd

Labor Standards in the United States and Canada

Block, Roberts, and Clarke offer a method for comparing ten labor standards across political jurisdictions. They then apply this method to the United States and Canada, an exercise that allows them to settle the long-running dispute over whether or not Canada has higher standards than the U.S., and if so, to what degree.https://research.upjohn.org/up_press/1050/thumbnail.jp

COMPARISON OF MODIFICATION SITES FORMED ON HUMAN SERUM ALBUMIN AT VARIOUS STAGES OF GLYCATION

Background—Many of the complications encountered during diabetes can be linked to the nonenzymatic glycation of proteins, including human serum albumin (HSA). However, there is little information regarding how the glycation pattern of HSA changes as the total extent of glycation is varied. The goal of this study was to identify and conduct a semi-quantitative comparison of the glycation products on HSA that are produced in the presence of various levels of glycation. Methods—Three glycated HSA samples were prepared in vitro by incubating physiological concentrations of HSA with 15 mmol/l glucose for 2 or 5 weeks, or with 30 mmol/l glucose for 4 weeks. These samples were then digested and examined by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify the glycation products that were formed. Results—It was found that the glycation pattern of HSA changed with its overall extent of total glycation. Many modifications including previously-reported primary glycation sites (e.g., K199, K281, and the N-terminus) were consistently found in the tested samples. Lysines 199 and 281, as well as arginine 428, contained the most consistently identified and abundant glycation products. Lysines 93, 276, 286, 414, 439, and 524/525, as well as the N-terminus and arginines 98, 197, and 521, were also found to be modified at various degrees of HSA glycation. Conclusions—The glycation pattern of HSA was found to vary with different levels of total glycation and included modifications at the 2 major drug binding sites on this protein. This result suggests that different modified forms of HSA, both in terms of the total extent of glycation and glycation pattern, may be found at various stages of diabetes. The clinical implication of these results is that the binding of HSA to some drug may be altered at various stages of diabetes as the extent of glycation and types of modifications in this protein are varied

Quantitative calculation of the role of the Na+,K+-ATPase in thermogenesis

AbstractThe Na+,K+-ATPase is accepted as an important source of heat generation (thermogenesis) in animals. Based on information gained on the kinetics of the enzyme's partial reactions we consider via computer simulation whether modifications to the function of the combined Na+,K+-ATPase/plasma membrane complex system could lead to an increased body temperature, either through the course of evolution or during an individual's lifespan. The enzyme's kinetics must be considered because it is the rate of heat generation which determines body temperature, not simply the amount of heat per enzymatic cycle. The results obtained indicate that a decrease in thermodynamic efficiency of the Na+,K+-ATPase, which could come about by Na+ substituting for K+ on the enzyme's extracellular face, could not account for increased thermogenesis. The only feasible mechanisms are an increase in the enzyme's expression level or an increase in its ion pumping activity. The major source of Na+,K+-ATPase-related thermogenesis (72% of heat production) is found to derive from passive Na+ diffusion into the cell, which counterbalances outward Na+ pumping to maintain a constant Na+ concentration gradient across the membrane. A simultaneous increase in both Na+,K+-ATPase activity and the membrane's passive Na+ permeability could promote a higher body temperature

Antibacterial Activity and Iron Release of Organic-Inorganic Hybrid Biomaterials Synthesized via the Sol-Gel Route

The aim of this work was the synthesis of hybrid materials of iron (II)-based therapeutic systems via the sol-gel method. Increasing amounts of polyethylene glycol (PEG 6, 12, 24, 50 wt%) were added to SiO2/Fe20 wt% to modulate the release kinetics of the drug from the systems. Fourier-transform infrared (FTIR) spectroscopy was used to study the interactions between different components in the hybrid materials. The release kinetics in a simulated body fluid (SBF) were investigated, and the amount of Fe2+ released was detected via ultraviolet-visible spectroscopy (UV-Vis) after reaction with ortho-phenanthroline. Furthermore, biological characterization was carried out. The bioactivity of the synthesized hybrid materials was evaluated via the formation of a layer of hydroxyapatite on the surface of samples soaked in SBF using spectroscopy. Finally, the potential antibacterial properties of seven different materials against two different bacteria—E. coli and S. aureus—were investigated