Genetic variability of meat quality traits in Chianina beef cattle

The heritability of quality traits (chemical composition, colour, tenderness and water-holding capacity) of the meat derivingfrom three muscles (Triceps brachii, Longissimus dorsi and Semitendinosus) was evaluated in 92 young Chianinabulls. The animals were raised on two farms with different feeding programs, and were slaughtered at approximately 19months of age. Single pair correlations were considered in order to evidence the relationships between the same traitsdetected in the three muscles and between all the traits of the same muscle.The h2 values range from 0.00 to 0.24 for the chemical composition, from 0.00 to 0.19 for colour parameters, from 0.03to 0.31 for those observed after 48 h, and from 0.00 to 0.08 for tenderness and water-holding capacity. The values werenot homogeneous for the three muscles. The most heritable trait was yellowness (b*48) in all three muscles, with valuesof 0.11 in the Longissimus dorsi, 0.23 in the Semitendinosus, and 0.31 in the Triceps brachii. Conversely, tendernessappeared to have a low heritability, as did the parameters relating to the water-holding capacity. Colour traitsobserved before and after 48 h of storage generally were significantly and positively correlated, indicating that certaincolour characteristics are maintained even after 48 h of storage. There was not always a close relationship between thetraits of each muscles: significant correlations existed between the Triceps brachii and the Longissimus dorsi muscles,confirming the similar quality traits of their meat, while there were considerable differences between these muscles andthe Semitendinosus

The genetic variability of the Podolica cattle breed from the Gargano area. Preliminary results

The Podolica cattle breed is autochthonous of Southern Italy and denoted by its particular rusticity. This study presents the preliminary results of the genetic characterization of the Podolica breed using DNA STR markers. A total of 20 microsatellite loci were analysed in 79 individuals reared in the Gargano area. Number of polymorphisms, allele fre- quencies, deviations from Hardy-Weinberg proportions, linkage disequilibrium between loci and genetic similarities between animals were calculated. The results showed a high deficiency of heterozygotes, the observed mean of het- erozygosis being 0.449, whereas the expected mean was 0.766. Many markers showed also deviations from the Hardy- Weinberg proportions and significant linkage disequilibrium between loci. However the genetic similarity within the pop- ulation was low (0.281) and the average number of alleles per locus was high (10), representing a high genetic vari- ability. In order to explain these results, a stratification of the breed in sub-populations with a high interior genetic homo- geneity but markedly differentiated one from each other could be hypothesized; this situation probably derived from non- random mating within each herd (consanguinity) and from the lack of exchange of genetic material between the herds. A further study is needed on a wider sample and extending the analysis to FAO-ISAG microsatellite panel in order to con- firm this hypothesis. This could eventually provide the information necessary for the correct management of the repro- ductive schemes and for genomic traceability of meat production

Analisi della variabilità genetica in una popolazione ovina di razza massese e studio di associazione con parametri di qualità del latte

RIASSUNTO Il presente lavoro ha come obiettivo la ricerca di associazioni tra marcatori microsatellite e la composizione chimica del latte. È stato condotto uno studio su 68 pecore di razza Massese provenienti da un allevamento toscano. A partire da latte fresco, sono stati valutati: composizione chimica standard, contenuto in caseina e frazioni caseiniche, proteine seriche, pH e parametri reologici. I capi sono stati sottoposti a genotipizzazione impiegando 17 marcatori microsatellite; sono, quindi, stati calcolati i valori di similarità genetica tra individui ed alcuni parametri genetici classici. Il numero medio di alleli per locus è risultato pari a 7,18 e l’eterozigosità osservata presentava variazioni tra 0,403 e 0,867 (media 0,677). La similarità genetica tra individui era 0,460. Cinque marcatori (BM8124, CSN3, BM1258, BMS468 e TGLA387) hanno mostrato una deviazione significativa dalle proporzioni di Hardy- Weinberg. Lo studio ha messo in evidenza alcuni microsatelliti con alleli significativamente associati a caratteri di composizione del latte (P<0,01). In particolare, la significatività più alta (P<0,001) è stata osservata per l’associazione dell’allele 2 del marcatore OIFNG con la a-lattoalbumina, dell’allele 10 del marcatore BL4 con il livello di immunoglobuline, dell’allele 2 del marcatore BMC1009 con il contenuto di grasso e dell’allele 9 del marcatore ILSTS42 con il parametro a30. Sono necessari ulteriori approfondimenti al fine di convalidare i risultati preliminari ottenuti nel presente lavoro e si rende necessaria l’estensione dello studio ad un numero maggiore di soggetti e la tipizzazione di un numero maggiore di loci, principalmente sui cromosomi 3 e 20 dove mappano i marcatori risultati più interessanti nella presente analisi. SUMMARY Current research aims to establish statistical associations between DNA microsatellites and milk chemical composition. Atrial was carried out on 68 Massese ewes reared in a farm of the Tuscany. The following parameters were evaluated on fresh milk: standard chemical composition, casein and its fractions, whey proteins, pH and rheological parameters. Animals were genotyped at 17 microsatellite loci. Genetic similarities among individuals and classical genetic parameters were evaluated. For each locus, average values of considered parameters were calculated in all the subjects carrying a given allele; these were compared statistically with the average values of subjects not carrying the allele, and the significance of the difference was estimated. The average number of alleles per locus resulted 7.18 and the observed heterozygosity ranged from 0.403 to 0.867 (0.677 medium value). The genetic similarity among individuals was 0.460. Five markers pointed out a significant deviation from the Hardy-Weinberg proportions (BM8124, CSN3, BM1258, BMS468 and TGLA387). The study revealed several microsatellites with alleles significantly linked to milk composition traits (P<0.01). In particular the highest significance (P<0.001) has been found for the allele 2 of OIFNG marker with a-lactoalbumin, for the allele 10 of the BL4 marker with immunoglobulins, for the allele 2 of BMC1009 with fat, and for allele 9 of ILSTS42 with a30. Further analyses are needed to validate these preliminary results, in particular increasing the number of subjects and of typed loci above all on the chromosomes 3 and 20 where the more interesting markers map

Genetic variability of three local cattle breeds (Calvana, Pontremolese, Garfagnina) by STR analysis

AbstractThe dramatic size contraction of local cattle breeds due to replacement with cosmopolite improved breeds highlights the need for native genetic resources conservation. In 1985, the Anagraphic Register of local cattle breeds and small-size ethnic groups was established by the Italian Ministry of Agriculture and Forestry. Calvana, Pontremolese and Garfagnina are among the included breeds. They are all native from Tuscany. Present breeding area covers the provinces of Firenze, Prato, Pistoia and Siena for the Calvana breed (around 280 heads), while it is restricted to the province of Lucca for both Garfagnina (around 180 heads) and Pontremolese. This latter breed consists, nowadays, of less than 40 heads, while being around 15000 in 1940s.The characterization of the genetic structure and variability via molecular markers could provide useful information for breed management and conservation. In the present study, a total of 149 animals, evenly distributed among the three breeds, were genetically char..

Development of a Nomogram Predicting the Risk of Persistence/Recurrence of Cervical Dysplasia

Background: Cervical dysplasia persistence/recurrence has a great impact on women's health and quality of life. In this study, we investigated whether a prognostic nomogram may improve risk assessment after primary conization. Methods: This is a retrospective multi-institutional study based on charts of consecutive patients undergoing conization between 1 January 2010 and 31 December 2014. A nomogram assessing the importance of different variables was built. A cohort of patients treated between 1 January 2015 and 30 June 2016 was used to validate the nomogram. Results: A total of 2966 patients undergoing primary conization were analyzed. The median (range) patient age was 40 (18-89) years. At 5-year of follow-up, 6% of patients (175/2966) had developed a persistent/recurrent cervical dysplasia. Median (range) recurrence-free survival was 18 (5-52) months. Diagnosis of CIN3, presence of HR-HPV types, positive endocervical margins, HPV persistence, and the omission of HPV vaccination after conization increased significantly and independently of the risk of developing cervical dysplasia persistence/recurrence. A nomogram weighting the impact of all variables was built with a C-Index of 0.809. A dataset of 549 patients was used to validate the nomogram, with a C-index of 0.809. Conclusions: The present nomogram represents a useful tool for counseling women about their risk of persistence/recurrence after primary conization. HPV vaccination after conization is associated with a reduced risk of CIN2+

How future surgery will benefit from SARS-COV-2-related measures: a SPIGC survey conveying the perspective of Italian surgeons

COVID-19 negatively affected surgical activity, but the potential benefits resulting from adopted measures remain unclear. The aim of this study was to evaluate the change in surgical activity and potential benefit from COVID-19 measures in perspective of Italian surgeons on behalf of SPIGC. A nationwide online survey on surgical practice before, during, and after COVID-19 pandemic was conducted in March-April 2022 (NCT:05323851). Effects of COVID-19 hospital-related measures on surgical patients' management and personal professional development across surgical specialties were explored. Data on demographics, pre-operative/peri-operative/post-operative management, and professional development were collected. Outcomes were matched with the corresponding volume. Four hundred and seventy-three respondents were included in final analysis across 14 surgical specialties. Since SARS-CoV-2 pandemic, application of telematic consultations (4.1% vs. 21.6%; p &lt; 0.0001) and diagnostic evaluations (16.4% vs. 42.2%; p &lt; 0.0001) increased. Elective surgical activities significantly reduced and surgeons opted more frequently for conservative management with a possible indication for elective (26.3% vs. 35.7%; p &lt; 0.0001) or urgent (20.4% vs. 38.5%; p &lt; 0.0001) surgery. All new COVID-related measures are perceived to be maintained in the future. Surgeons' personal education online increased from 12.6% (pre-COVID) to 86.6% (post-COVID; p &lt; 0.0001). Online educational activities are considered a beneficial effect from COVID pandemic (56.4%). COVID-19 had a great impact on surgical specialties, with significant reduction of operation volume. However, some forced changes turned out to be benefits. Isolation measures pushed the use of telemedicine and telemetric devices for outpatient practice and favored communication for educational purposes and surgeon-patient/family communication. From the Italian surgeons' perspective, COVID-related measures will continue to influence future surgical clinical practice

Infected pancreatic necrosis: outcomes and clinical predictors of mortality. A post hoc analysis of the MANCTRA-1 international study

: The identification of high-risk patients in the early stages of infected pancreatic necrosis (IPN) is critical, because it could help the clinicians to adopt more effective management strategies. We conducted a post hoc analysis of the MANCTRA-1 international study to assess the association between clinical risk factors and mortality among adult patients with IPN. Univariable and multivariable logistic regression models were used to identify prognostic factors of mortality. We identified 247 consecutive patients with IPN hospitalised between January 2019 and December 2020. History of uncontrolled arterial hypertension (p = 0.032; 95% CI 1.135-15.882; aOR 4.245), qSOFA (p = 0.005; 95% CI 1.359-5.879; aOR 2.828), renal failure (p = 0.022; 95% CI 1.138-5.442; aOR 2.489), and haemodynamic failure (p = 0.018; 95% CI 1.184-5.978; aOR 2.661), were identified as independent predictors of mortality in IPN patients. Cholangitis (p = 0.003; 95% CI 1.598-9.930; aOR 3.983), abdominal compartment syndrome (p = 0.032; 95% CI 1.090-6.967; aOR 2.735), and gastrointestinal/intra-abdominal bleeding (p = 0.009; 95% CI 1.286-5.712; aOR 2.710) were independently associated with the risk of mortality. Upfront open surgical necrosectomy was strongly associated with the risk of mortality (p &lt; 0.001; 95% CI 1.912-7.442; aOR 3.772), whereas endoscopic drainage of pancreatic necrosis (p = 0.018; 95% CI 0.138-0.834; aOR 0.339) and enteral nutrition (p = 0.003; 95% CI 0.143-0.716; aOR 0.320) were found as protective factors. Organ failure, acute cholangitis, and upfront open surgical necrosectomy were the most significant predictors of mortality. Our study confirmed that, even in a subgroup of particularly ill patients such as those with IPN, upfront open surgery should be avoided as much as possible. Study protocol registered in ClinicalTrials.Gov (I.D. Number NCT04747990)