Mitigating odors from animal facilities using biofilters

Mitigating odors from livestock sites using biofilters was addressed in this dissertation which is organized in paper format and comprises a literature review paper and two original research papers. In the first literature review paper, both the laboratory and field research from 1997 to 2008 was reviewed to give an up-to-date perspective of studies on the mitigation of odors and volatile organic compounds (VOCs) relating to agricultural facilities using biofilters. A mobile biofiter testing system was developed which can be used at field conditions to evaluate biofilter performance at variable media types, empty bed residence times (EBRT) and media moisture contents. A field study for mitigating odors and odorous compounds from a deep-pit swine finishing facility in central Iowa was conducted to test two types of wood chip media (western cedar and two inch hardwood) using the mobile biofilter testing system. The biofilter testing system was continuously operated and evaluated at different media moisture content levels and different air flow rates resulting in a variable EBRT from 1.6 to 7.3 sec. A dynamic forced-choice olfactometer was used to evaluate odor concentrations from both the control plenum and biofilter treatments. Hydrogen sulfide and ammonia concentrations were also measured from these olfactometry samples. Solid-phase microextraction fibers were used to extract VOCs from both the control plenum and biofilter treatments. Analyses of VOCs and its corresponding individual odors were carried out using a gas chromatography-mass spectrometry-olfactometry (GC-MS-O) system. Pressure drop characteristics through the biofilters packed with western cedar and hardwood media were monitored. The reduction efficiency and pressure drop characteristics obtained with the wood chip-based biofilters indicate the feasibility of farm-level applications of wood chip-based biofilters for reducing swine building odors. The results also showed that both a proper media moisture content and a minimum EBRT were critical to a successful wood chip-based biofilter

Electronic structure and bonding properties of cobalt oxide in the spinel structure

The spinel cobalt oxide Co3O4 is a magnetic semiconductor containing cobalt ions in Co2+ and Co3+ oxidation states. We have studied the electronic, magnetic and bonding properties of Co3O4 using density functional theory (DFT) at the Generalized Gradient Approximation (GGA), GGA+U, and PBE0 hybrid functional levels. The GGA correctly predicts Co3O4 to be a semiconductor, but severely underestimates the band gap. The GGA+U band gap (1.96 eV) agrees well with the available experimental value (~ 1.6 eV), whereas the band gap obtained using the PBE0 hybrid functional (3.42 eV) is strongly overestimated. All the employed exchange-correlation functionals predict 3 unpaired d electrons on the Co2+ ions, in agreement with crystal field theory, but the values of the magnetic moments given by GGA+U and PBE0 are in closer agreement with the experiment than the GGA value, indicating a better description of the cobalt localized d states. Bonding properties are studied by means of Maximally Localized Wannier Functions (MLWFs). We find d-type MLWFs on the cobalt ions, as well as Wannier functions with the character of sp3d bonds between cobalt and oxygen ions. Such hybridized bonding states indicate the presence of a small covalent component in the primarily ionic bonding mechanism of this compound.Comment: 24 pages, 8 figure

First principles study of intrinsic point defects in hexagonal barium titanate

Density functional theory (DFT) calculations have been used to study the nature of intrinsic defects in the hexagonal polymorph of barium titanate. Defect formation energies are derived for multiple charge states and due consideration is given to finite-size effects (elastic and electrostatic) and the band gap error in defective cells. Correct treatment of the chemical potential of atomic oxygen means that it is possible to circumvent the usual errors associated with the inaccuracy of DFT calculations on the oxygen dimer. Results confirm that both mono- and di-vacancies exist in their nominal charge states over the majority of the band gap. Oxygen vacancies are found to dominate the system in metal-rich conditions with face sharing oxygen vacancies being preferred over corner sharing oxygen vacancies. In oxygen-rich conditions, the dominant vacancy found depends on the Fermi level. Binding energies also show the preference for metal-oxygen di-vacancy formation. Calculated equilibrium concentrations of vacancies in the system are presented for numerous temperatures. Comparisons are drawn with the cubic polymorph as well as with previous potential-based simulations and experimental results

Krüppel-like factor 8 promotes aerobic glycolysis in prostate cancer cells by regulating AKT/mTOR signaling pathway

Purpose: To investigate the effects of Krüppel-like factor 8 (KLF8) in prostate cancer (PCa) cell viability and glycolysis, and explore its role as a regulatory factor.Methods: Immunoblot assays were conducted to assess the expression of KLF8 and proteins in AKT/mTOR pathway in PCa cell lines PC-3 and DU145. Cell Counting Kit-8 assays were performed to assess the effect of KLF8 on PCa cell viability. The glycolysis capacity of PCa cells was determined by measuring the levels of glucose intake, lactic acid production, and cellular ATP levels.Results: Depletion of KLF8 decreased the survival of PCa cells in vitro (p < 0.05). KLF8 depletion also inhibited aerobic glucose metabolism in PCa cells (p < 0.05). Further studies confirmed that KLF8 contributed to the growth and glycolysis of PCa cells via the regulation of AKT/mTOR pathway.Conclusion: KLF8 regulates glycolysis in PCa cells by regulating AKT/mTOR signaling pathway and is thus a promising therapeutic target for PCa treatment. Keywords: Krüppel-like factor 8 (KLF8), Prostate cancer (PCa), Aerobic glucose, AKT/mTOR signaling pathway, Therapeutic targe

Assessment of a Two-Stage Wood Chip-Based Biofilter Using Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry-Olfactometry

A mobile pilot-scale biofilter testing laboratory, which consisted of one- and two-stage biofilter reactor barrels, was developed where two types of wood chips (western cedar and hard wood) were examined to treat odor emissions from a deep-pit swine finishing facility in central Iowa. The biofilters were operated continuously from July 20 to October 17, 2007 at differe nt air flow rates resulting in variable empty bed residence times. During this test period, solid-phase microextraction 85 µm Carboxen/PDMS fibers were used to extract volatile organic compounds (VOCs) from both the control plenum and biofilter treatment. Analyses of VOCs were carried out using a multidimensional gas chromatography-mass spectrometry-olfactometry system. Reductions of nine odorous compounds were reported. An overall average reduction efficiency of 98.9% and 96.4% was achieved for two-stage western cedar and hardwood biofilters, respectively. The results showed that maintaining proper moisture content is critical to the success of wood chip-based biofilter