46 research outputs found

    Nucleosome conformation dictates the histone code

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    Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized 'codes' that are read by specialized regions (reader domains) in chromatin-associated proteins (CAPs) to regulate downstream function. Substantial effort has been made to define [CAP: histone PTM] specificities, and thus decipher the histone code and guide epigenetic therapies. However, this has largely been done using the reductive approach of isolated reader domains and histone peptides, which cannot account for any higher-order factors. Here, we show that the [BPTF PHD finger and bromodomain: histone PTM] interaction is dependent on nucleosome context. The tandem reader selectively associates with nucleosomal H3K4me3 and H3K14ac or H3K18ac, a combinatorial engagement that despite being in cis is not predicted by peptides. This in vitro specificity of the BPTF tandem reader for PTM-defined nucleosomes is recapitulated in a cellular context. We propose that regulatable histone tail accessibility and its impact on the binding potential of reader domains necessitates we refine the 'histone code' concept and interrogate it at the nucleosome level

    State of the world’s plants and fungi 2020

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    Kew’s State of the World’s Plants and Fungi project provides assessments of our current knowledge of the diversity of plants and fungi on Earth, the global threats that they face, and the policies to safeguard them. Produced in conjunction with an international scientific symposium, Kew’s State of the World’s Plants and Fungi sets an important international standard from which we can annually track trends in the global status of plant and fungal diversity

    Gaia early data release 3: summary of the contents and survey properties (Corrigendum)

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    ERRATUMThis article is an erratum for:[https://doi.org/10.1051/0004-6361/202039657]​​​​​​​Instrumentatio

    Gaia Early Data Release 3: acceleration of the solar system from Gaia astrometry

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    Stars and planetary system

    Gaia Data Release 3: reflectance spectra of Solar System small bodies

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    Stars and planetary system

    Gaia Data Release 3: the extragalactic content

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    Galaxie

    Gaia Early Data Release 3: the Gaia catalogue of nearby stars

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    Stars and planetary system

    Gaia focused product release: radial velocity time series of long-period variables

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    Stars and planetary system

    Gaia Data Release 3: mapping the asymmetric disc of the Milky Way

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    Galaxie
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