Use of five probiotic strains to determine sensitivity in vitro on pathogenic bacteria growth isolated from sick fishes

ABSTRACT Ornamental aquaculture is an activity in clear economic growth, both globally and in Mexico where the development is particularly relevant to freshwater species. Infectious diseases produced by fungus, bacteria and virus are considered one of the principal limitations during the productive process. Between implemented strategies for reduction of antibiotic use, which are "living microorganisms that confer a health benefit to the host if they are given in adequate quantities"; lactic acid bacteria and yeast are among the most common used microorganism in aquaculture. This investigation, prove the effect of isolated probiotic bacteria from the digestive tract of healthy fish, belonging to specie: Bacillus sp., Bacillus laterosporus, Bacillus subtilis, Lactobacillus sp, and Lactococcus lactis, at different dilutions (10 9 ,10 8 , 10 7 ,10 6 , 10 5 and 10 4 ) in vitro growth of pathogenic bacteria: Citrobacter freundii, Enterobacter sakasakii, Klebsiella oxytoca, Proteus vulgaris, and Vibrio fluvialis, isolated from kidney of sick fish, cultured and purified through successive inoculations and identificated by the amplification of gene 16S of rRNA (PCR) using universal primers 8 for. (5'-AGACTTTGATCATGGCTCAG-3') and 1492 rev. (5'-TACGGCTACCTTGTTACGACTT-3') and comparison with GENEBANK sequences base. Probiotic strains were previously isolated from the digestive tract of different healthy fish in the laboratory. In order to perform in vitro challenge tests, pathogenic strains were inoculated three times each in BHI agar boxes at a concentration of 1x10 7 CFU mL -1 and subsequently using the well diffusion method, 70 µL from a suspension with each of the probiotic strains were added. Agar boxes were incubated 24 h at 30ºC to observe the formation of inhibition halos. Obtained values from inhibition halos were transformed to qualitative data with the following premise: halo diameter < 2.0 mm negative effect; halo diameter > 2.0 mm positive effect. In this study, it was determined that probiotic strains B. subtilis was the one that gave better results to inhibit the growth of pathogenic bacteria P. vulgaris, E. sakazakii, V. fluvialis, K. oxytoca and C. freundii in most of used dilutions. Making it a strain with high potential in aquaculture. Key words: Growth, halos, probiotics, bacteria, sensibility. RESUMEN La acuicultura de especies ornamentales es una actividad económica en franco crecimiento, tanto a nivel mundial como en México, en donde tiene particular desarrollo lo correspondiente a especies dulceacuícolas. Las enfermedades infeccionas producidas por hongos, bacterias y virus, están consideradas una de las limitantes principales durante el proceso productivo. Entre las estrategias implementadas para disminuir el uso de antibióticos para el control de patógenos, se encuentra el control biológico mediante el uso de organismos probióticos, los cuales son "microorganismos vivos los cuales, administrados en cantidades adecuadas, confieren un beneficio en la salud del hospedador"; entre los de uso más común en acuicultura se encuentran las lactobacterias y las levaduras. En el presente trabajo, se probó el efecto de bacterias probióticas aisladas del tracto digestivo de peces sanos, pertenecientes a las especies: Bacillus sp., Bacillus laterosporus, Bacillus subtilis, Lactobacillus sp, y Lactococcus lactis, a diferentes diluciones (10 9 ,10 8 , 10 7 ,10 6 , 10 5 y 10 4 ) en el crecimiento in vitro de las bacterias patógenas: Citrobacter freundii, Enterobacter sakasakii, Klebsiella oxytoca, Proteus vulgaris y Vibrio fluvialis, aisladas del riñón de peces enfermos, cultivadas y purificadas a través de resiembras sucesivas e identificadas mediante la amplificación del gen 16S del ARNr (PCR) utilizando los primers universales 8 for. 24 AGACTTTGATCATGGCTCAG-3') y 1492 rev. (5'-TACGGCTACCTTGTTACGACTT-3') y su comparación con la base de secuencias GENEBANK. Las cepas probióticos fueron aisladas previamente del tracto intestinal de diversos peces sanos en el laboratorio. Para llevar a cabo las pruebas de desafío in vitro, las cepas patógenas se sembraron por triplicado en cajas de agar BHI a una concentración de 1x10 7 UFC mL -1 y posteriormente, utilizando el método de difusión en pozos, se adicionaran 70 µL de una suspensión con cada una de las cepas probióticas Las placas se incubaron durante 24 h a 30ºC para observar la formación de halos de inhibición. Los valores obtenidos de los halos de inhibición fueron transformados a datos cualitativos con la siguiente premisa: diámetro halo < 2.0 mm efecto negativo; diámetro de halo > 2.0 mm efecto positivo. En este estudio, se determinó que la cepas probiótica B. subtilis fue la que dio mejores resultados al inhibir el crecimiento de las bacterias patógenas P. vulgaris, E. sakazakii, V. fluvialis, K. oxytoca y C. freundii en la mayoría de las diluciones utilizadas. Por lo que es una cepa con alto potencial en acuicultura

Factors that promote positive attitudes towards mathematics in higher education students

The purpose of this review article is to explain the importance of understanding attitudes towards teaching and learning mathematics in higher education students. For that, we start from the premise that attitudes are related to performance towards learning mathematics, bearing in mind that what directly influences their success are the feelings of possessing competencies to understand their contents and not the feelings of difficulty towards them. In essence, at first, we describe the different attitudes towards mathematics. Next, we examine how attitudes influence math learning and finally identify the factors that foster positive attitudes toward math. From this, we suggest teaching practices that can be carried out to foster positive attitudes towards mathematics and propose new lines of research in this field

Impact of a basic mathematics course on the performance of college students in algebra subject

A common problem for universities is the lack of knowledge and mathematical skills of new students because this deficiency creates difficulties in their studies throughout their career, which can cause their dropout. The aim of this paper is to describe the impact of a basic mathematics course on the performance of college student in algebra subject. This is a 32-hour course which is developed in 16 sessions in parallel to the algebra classes and is mainly offered to students: a) identified with low performance on mathematics component of national highschool test, and b) remitted by the subject teacher. The main findings are: a) the pass rate for students who attended to more than half of the sessions of the course if 95%, and b) the average grade of the subject tends to increase as with the attendance level to the course of the students. Consequently, these results lead to the conclusion that the course has a positive effect on the students' performance

Innovation and growth in family-owned Mexican SMEs

This paper studies the relationship between innovation and the level of growth in family-owned small and medium-sized enterprises (SMEs), this is done within the context of Mexico. The conceptual framework of analysis is based on the hypothesis that innovation in products, processes and management systems contribute to the achievement of growth in family-owned SMEs. To test this hypothesis, a model of structural equation of second order was developed with data collected from 206 family-owned SMEs located in the state of Aguascalientes, Mexico. The empirical evidence provided by the analysis supports the hypothesis, showing that family-owned SMEs that increase their innovation activities will also significantly increase their opportunities for growth. This finding can be used to inform the business growth strategies formulated by the owners, or managers, of family-owned SEMs. These also indicate policy-makers that by designing effective policies and providing incentives to support innovation in family-owned SMEs, the government would not only contribute to the growth of these organisations but also to the growth of their regions and countries. Despite the importance of innovation and family-owned SMEs, evidence suggests that studies focused on investigating innovation in this type of organisations are very limited. This paper fills this gap by providing a refined understanding of the relationship between innovation and growth in family-owned SMEs

Experimental evidence of icosahedral and decahedral packing in one-dimensional nanostructures

Abstract: The packing of spheres is a subject that has drawn the attention of mathematicians and philosophers for centuries and that currently attracts the interest of the scientific community in several fields. At the nanoscale, the packing of atoms affects the chemical and structural properties of the material and, hence, its potential applications. This report describes the experimental formation of 5-fold nanostructures by the packing of interpenetrated icosahedral and decahedral units. These nanowires, formed by the reaction of a mixture of metal salts (Au and Ag) in the presence of oleylamine, are obtained when the chemical composition is specifically Ag/Au = 3:1. The experimental images of the icosahedral nanowires have a high likelihood with simulated electron micrographs of structures formed by two or three Boerdijk Coxeter Bernal helices roped on a single structure, whereas for the decahedral wires, simulations using a model of adjacent decahedra match the experimental structures. To our knowledge, this is the first report of the synthesis of nanowires formed by the packing of structures with 5-fold symmetry. These icosahedral nanowire structures are similar to those of quasicrystals, which can only be formed if at least two atomic species are present and in which icosahedral and decahedral packing has been found for bulk crystals

VOLUMEN 15, NÚMERO 29 (1981-1982)

USO DEL COMPUTADOR EN EL ANÁLISIS SEDIMENTOLÓGICO Y DE MIGRACIÓN DE FLUIDOS Y SU APLICACIÓN EN EL NOROCCIDENTE COLOMBIANO. Castro, A. E.; Molina, G. J.UN MODELO ESTRUCTURAL PARA EL VALLE DEL CAUCA Y SUS IMPLICACIONES HIDROGEOLÓGICAS Y MINERAS. Padilla, L. E.TECTÓNICA DE SOBRECORRIMIENTO Y SUS IMPLICACIONES ESTRUCTURALES EN EL ÁREA PAMPLONA - CORDILLERA ORIENTAL DE COLOMBIA. Boinet, T.; Bourgois, J.; Mendoza F. HGEOLOGÍA DE LAS LATERITAS NIQUELÍFERAS DE CERRO MATOSO S. A. Mejía, V. M.; Durango, J. R.COMPLEJO OFIOLÍTICO EN LA CUENCA DEL RÍO GUAPI. Ortega Montero, C.DETERMINACIÓN DE RESERVAS DE DEPÓSITOS MINERALES. Hernández Garay, H.CARACTERIZACIÓN, RANGO Y CLASIFICACIÓN DE CARBONES. Ramírez Castro, P

VOLUMEN 15, NÚMERO 29 (1981-1982)

USO DEL COMPUTADOR EN EL ANÁLISIS SEDIMENTOLÓGICO Y DE MIGRACIÓN DE FLUIDOS Y SU APLICACIÓN EN EL NOROCCIDENTE COLOMBIANO. Castro, A. E.; Molina, G. J.UN MODELO ESTRUCTURAL PARA EL VALLE DEL CAUCA Y SUS IMPLICACIONES HIDROGEOLÓGICAS Y MINERAS. Padilla, L. E.TECTÓNICA DE SOBRECORRIMIENTO Y SUS IMPLICACIONES ESTRUCTURALES EN EL ÁREA PAMPLONA - CORDILLERA ORIENTAL DE COLOMBIA. Boinet, T.; Bourgois, J.; Mendoza F. HGEOLOGÍA DE LAS LATERITAS NIQUELÍFERAS DE CERRO MATOSO S. A. Mejía, V. M.; Durango, J. R.COMPLEJO OFIOLÍTICO EN LA CUENCA DEL RÍO GUAPI. Ortega Montero, C.DETERMINACIÓN DE RESERVAS DE DEPÓSITOS MINERALES. Hernández Garay, H.CARACTERIZACIÓN, RANGO Y CLASIFICACIÓN DE CARBONES. Ramírez Castro, P

Analysis of the Relationship between Lifestyle and Coffee Consumption Habits, from the Myth Approach, in the Municipalities of Orizaba, Tehuipango, and Zongolica Veracruz.

Coffee consumption habits have evolved along with society for more than 300 years around the world. Social changes imply different lifestyles in each culture. Lifestyles influence the myths used by people in their consumption decisions. The objective of this research is to determine the relationship between lifestyle and coffee consumption habits, from the perspective of myth, in the inhabitants of the Mexican municipalities of Orizaba, Tehuipango, and Zongolica. A survey was conducted with a sample of inhabitants of the three municipalities studied. The statistical analyzes applied were the central limit theorem, Pearson, and Chi-square. The results show that the variables Consumption habits-Lifestyle-Myth, are highly dependent on the level of perception of people. It is concluded that the coffee myth corresponds to the "family union" in the study municipalities. This myth is not characterized in the evolutionary stages contemplated in the "waves of coffee"

The burden of 14 hr-HPV genotypes in women attending routine cervical cancer screening in 20 states of Mexico: a cross-sectional study

In Mexico, HPV vaccines available immunize against genotypes 16/18 and 16/18/6/11; however, there is limited surveillance about carcinogenic subtypes in different states of the country that allow evaluating the effectiveness of vaccination and cervical cancer screening programs. Here, we report the regional and age-specific prevalence of 14 hr-HPV genotypes as well as their prevalence in abnormal cytology (from ASCUS to cervical cancer) among Mexican women which were undergoing from cervical cancer screening in the Salud Digna clinics in 20 states of the country. This study includes women with social security from the majority of public health institutions (IMSS, ISSSTE, SEMAR, and PEMEX), and women without social security. For cervical cancer screening, we used the SurePath liquid-based cytology and the BD Onclarity HPV Assay. From December 1, 2016, to August 2, 2018, the hr-HPV prevalence among 60,135 women was 24.78%, the most prevalent types were HPV 16 (4.13%), HPV 31 (4.12%) and HPV 51 (3.39%), while HPV 18 (1.70%) was less prevalent among infected women. Interestingly, the genotypes not covered by current vaccines in Mexico were commonly found in precancerous lesions, evidencing their carcinogenic potential, so it is necessary to increase their surveillance and inclusion in cervical cancer screening triage.We gratefully acknowledge to Iromy Meza, Jessica Avitia, and Oswaldo Carrillo for their technical support in obtaining databases during this project. Also, we want to thanks the staff of the Salud Digna clinics and the National Reference Center of Salud Digna for their support during this work. This work was funding by Salud Digna