Differential expression of 12 histone deacetylase (HDAC) genes in astrocytomas and normal brain tissue: class II and IV are hypoexpressed in glioblastomas

<p>Abstract</p> <p>Background</p> <p>Glioblastoma is the most lethal primary malignant brain tumor. Although considerable progress has been made in the treatment of this aggressive tumor, the clinical outcome for patients remains poor. Histone deacetylases (HDACs) are recognized as promising targets for cancer treatment. In the past several years, HDAC inhibitors (HDACis) have been used as radiosensitizers in glioblastoma treatment. However, no study has demonstrated the status of global <it>HDAC </it>expression in gliomas and its possible correlation to the use of HDACis. The purpose of this study was to evaluate and compare mRNA and protein levels of class I, II and IV of HDACs in low grade and high grade astrocytomas and normal brain tissue and to correlate the findings with the malignancy in astrocytomas.</p> <p>Methods</p> <p>Forty-three microdissected patient tumor samples were evaluated. The histopathologic diagnoses were 20 low-grade gliomas (13 grade I and 7 grade II) and 23 high-grade gliomas (5 grade III and 18 glioblastomas). Eleven normal cerebral tissue samples were also analyzed (54 total samples analyzed). mRNA expression of class I, II, and IV <it>HDACs </it>was studied by quantitative real-time polymerase chain reaction and normalized to the housekeeping gene <it>β-glucuronidase</it>. Protein levels were evaluated by western blotting.</p> <p>Results</p> <p>We found that mRNA levels of class II and IV <it>HDACs </it>were downregulated in glioblastomas compared to low-grade astrocytomas and normal brain tissue (7 in 8 genes, <it>p </it>< 0.05). The protein levels of class II HDAC9 were also lower in high-grade astrocytomas than in low-grade astrocytomas and normal brain tissue. Additionally, we found that histone H3 (but not histone H4) was more acetylated in glioblastomas than normal brain tissue.</p> <p>Conclusion</p> <p>Our study establishes a negative correlation between <it>HDAC </it>gene expression and the glioma grade suggesting that class II and IV <it>HDACs </it>might play an important role in glioma malignancy. Evaluation of histone acetylation levels showed that histone H3 is more acetylated in glioblastomas than normal brain tissue confirming the downregulation of <it>HDAC </it>mRNA in glioblastomas.</p

ARDD 2020: from aging mechanisms to interventions

Aging is emerging as a druggable target with growing interest from academia, industry and investors. New technologies such as artificial intelligence and advanced screening techniques, as well as a strong influence from the industry sector may lead to novel discoveries to treat age-related diseases. The present review summarizes presentations from the 7th Annual Aging Research and Drug Discovery (ARDD) meeting, held online on the 1st to 4th of September 2020. The meeting covered topics related to new methodologies to study aging, knowledge about basic mechanisms of longevity, latest interventional strategies to target the aging process as well as discussions about the impact of aging research on society and economy. More than 2000 participants and 65 speakers joined the meeting and we already look forward to an even larger meeting next year. Please mark your calendars for the 8th ARDD meeting that is scheduled for the 31st of August to 3rd of September, 2021, at Columbia University, USA

An RFID-Based Digital Bracelet for the Timely Assistance of Indigents

High rates of indigence characterize several countries in Latin America, such as Mexico. In the north of our country, the phenomenon of migration contributes to indigence. To get a preliminary understanding of the needs of indigents in our community, we conducted interviews with homeless and migrants. This helped us identify preliminary system requirements that led to the design of a digital bracelet to enable local agencies to monitor and provide support to the indigents who roam the city. We refined our conceptual design through an iterative process of contextual inquiry, persona development, prototyping, and user assessment. An evaluation of our design was conducted with the coordinator of the main social assistance agency of the municipality, which allowed us to confirm the system potential to be used and adopted

Combined Borehole Seismic and Electromagnetic Inversion For High-Resolution Petrophysical Assessment Of Hydocarbon Reservoirs

This report summarizes the work performed between January 2005 and December 2007, under DOE research contract DE-FC26-04NT15507. The project is was performed by the Center for Petroleum and Geosystems Engineering of The University of Texas at Austin and Lawrence Berkeley National Laboratory under the auspices of the National Energy Technology Office (NETL) and the Strategic Center for Natural Gas and Oil (SCNGO). During the three-year project, we developed new methods to combine borehole sonic and electromagnetic (EM) measurements for the improved assessment of elastic and petrophysical properties of rock formations penetrated by a well. Sonic measurements consisted of full waveform acoustic amplitudes acquired with monopole and dipole sources, whereas EM measurements consisted of frequency-domain voltages acquired with multi-coil induction systems. The combination of sonic and EM measurements permitted the joint estimation of elastic and petrophysical properties in the presence of mud-filtrate invasion. It was conclusively shown that the combined interpretation of sonic and EM measurements reduced non-uniqueness in the estimation of elastic and petrophysical properties and improved the spatial resolution of the estimations compared to estimations yielded separately from the two types of measurements. Moreover, this approach enabled the assessment of dynamic petrophysical properties such as permeability, as it incorporated the physics of mud-filtrate invasion in the interpretation of the measurements. The first part of the project considered the development of fast and reliable numerical algorithms to simulate borehole sonic waveforms in 2D, 3D, and radial 1D media. Such algorithms were subsequently used in the quantitative estimation of elastic properties jointly from borehole sonic and EM measurements. In the second part of the project we developed a new algorithm to estimate water saturation, porosity, and dry-rock elastic moduli jointly from borehole sonic and EM measurements. This algorithm assumed radial 1D variations of fluid saturation due to mud-filtrate invasion. Subsequently, we adapted the estimation method to interpret borehole field measurements acquired in both a shaly-sand sedimentary sequence and a tight-gas sandstone formation. In the two cases, we simulated the process of mud-filtrate invasion and concomitantly honored sonic and EM measurements. We produced reliable estimates of permeability and dry-rock moduli that were successfully validated with rock-core measurements. Finally, we introduced a new stochastic inversion procedure to estimate elastic, electrical, and petrophysical properties of layered media jointly from waveform sonic and frequency-domain EM measurements. The procedure was based on Bayesian statistical inversion and delivered estimates of uncertainty under various forms of a-priori information about the unknown properties. Tests on realistic synthetic models confirmed the reliability of this procedure to estimate elastic and petrophysical properties jointly from sonic and EM measurements. Several extended abstracts and conference presentations stemmed from this project, including 2 SEG extended abstracts, 1 SPE extended abstract, and 2 SPWLA extended abstracts. Some of these extended abstracts have been submitted for publication in peer-reviewed journals

CONCURRENCY AND COMPUTATION: PRACTICE AND EXPERIENCE

Full waveform seismic inversion using a distributed system of computer

ARDD 2020: from aging mechanisms to interventions

Aging is emerging as a druggable target with growing interest from academia, industry and investors. New technologies such as artificial intelligence and advanced screening techniques, as well as a strong influence from the industry sector may lead to novel discoveries to treat age-related diseases. The present review summarizes presentations from the 7th Annual Aging Research and Drug Discovery (ARDD) meeting, held online on the 1st to 4th of September 2020. The meeting covered topics related to new methodologies to study aging, knowledge about basic mechanisms of longevity, latest interventional strategies to target the aging process as well as discussions about the impact of aging research on society and economy. More than 2000 participants and 65 speakers joined the meeting and we already look forward to an even larger meeting next year. Please mark your calendars for the 8th ARDD meeting that is scheduled for the 31st of August to 3rd of September, 2021, at Columbia University, USA.ISSN:1945-458

ARDD 2020: from aging mechanisms to interventions

Aging is emerging as a druggable target with growing interest from academia, industry and investors. New technologies such as artificial intelligence and advanced screening techniques, as well as a strong influence from the industry sector may lead to novel discoveries to treat age-related diseases. The present review summarizes presentations from the 7th Annual Aging Research and Drug Discovery (ARDD) meeting, held online on the 1st to 4th of September 2020. The meeting covered topics related to new methodologies to study aging, knowledge about basic mechanisms of longevity, latest interventional strategies to target the aging process as well as discussions about the impact of aging research on society and economy. More than 2000 participants and 65 speakers joined the meeting and we already look forward to an even larger meeting next year. Please mark your calendars for the 8th ARDD meeting that is scheduled for the 31st of August to 3rd of September, 2021, at Columbia University, USA

ARDD 2020: from aging mechanisms to interventions.

Aging is emerging as a druggable target with growing interest from academia, industry and investors. New technologies such as artificial intelligence and advanced screening techniques, as well as a strong influence from the industry sector may lead to novel discoveries to treat age-related diseases. The present review summarizes presentations from the 7th Annual Aging Research and Drug Discovery (ARDD) meeting, held online on the 1st to 4th of September 2020. The meeting covered topics related to new methodologies to study aging, knowledge about basic mechanisms of longevity, latest interventional strategies to target the aging process as well as discussions about the impact of aging research on society and economy. More than 2000 participants and 65 speakers joined the meeting and we already look forward to an even larger meeting next year. Please mark your calendars for the 8th ARDD meeting that is scheduled for the 31st of August to 3rd of September, 2021, at Columbia University, USA