Commentary on: Schuman MJ, Hutchins KD. Severe retinal hemorrhages with retinoschisis in infants are not pathognomonic for abusive head trauma. J Forensic Sci 2017;62(3):807–11.

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Marek's Disease Virus VP22: Subcellular Localization and Characterization of Carboxyl Terminal Deletion Mutations

AbstractMarek's disease virus (MDV) is an alphaherpesvirus that causes T cell lymphoma and severe immunosuppression in chickens. The MDV UL49 gene, which encodes the tegument viral protein 22 (VP22), has been expressed as a green fluorescent protein (GFP) fusion protein in chicken embryonic fibroblasts to examine its subcellular localization. As with both human herpesvirus 1 and bovine herpesvirus 1VP22-GFP fusion proteins, the MDV VP22-GFP product binds to microtubules and heterochromatin. In addition, the MDV protein also binds to the centrosomes. During mitosis, VP22-GFP binds to sister chromatids, but dissociates from the centrosomes and the microtubules of the mitotic spindle. A series of VP22 carboxy terminal truncation mutants were constructed to define regions responsible for these binding properties. These mutants identified separable domains or motifs responsible for binding microtubules and heterochromatin

Gallus GBrowse: a unified genomic database for the chicken

Gallus GBrowse (http://birdbase.net/cgi-bin/gbrowse/gallus/) provides online access to genomic and other information about the chicken, Gallus gallus. The information provided by this resource includes predicted genes and Gene Ontology (GO) terms, links to Gallus In Situ Hybridization Analysis (GEISHA), Unigene and Reactome, the genomic positions of chicken genetic markers, SNPs and microarray probes, and mappings from turkey, condor and zebra finch DNA and EST sequences to the chicken genome. We also provide a BLAT server (http://birdbase.net/cgi-bin/webBlat) for matching user-provided sequences to the chicken genome. These tools make the Gallus GBrowse server a valuable resource for researchers seeking genomic information regarding the chicken and other avian species

The Transcription Factor NURR1 Exerts Concentration-Dependent Effects on Target Genes Mediating Distinct Biological Processes

The transcription factor NURR1 plays a pivotal role in the development and maintenance of neurotransmitter phenotype in midbrain dopamine neurons. Conversely, decreased NURR1 expression is associated with a number of dopamine-related CNS disorders, including Parkinson’s disease and drug addiction. In order to better understand the nature of NURR1-responsive genes and their potential roles in dopamine neuron differentiation and survival, we used a human neural cellular background (SK-N-AS cells) in which to generate a number of stable clonal lines with graded NURR1 gene expression that approximated that seen in DA cell-rich human substantia nigra. Gene expression profiling data from these NURR1-expressing clonal lines were validated by quantitative RT-PCR and subjected to bioinformatic analyses. The present study identified a large number of NURR1-responsive genes and demonstrated the potential importance of concentration-dependent NURR1 effects in the differential regulation of distinct NURR1 target genes and biological pathways. These data support the promise of NURR1-based CNS therapeutics for the neuroprotection and/or functional restoration of DA neurons

Measuring Channel Planform Change From Image Time Series: A Generalizable, Spatially Distributed, Probabilistic Method for Quantifying Uncertainty

Abstract Channels change in response to natural or anthropogenic fluctuations in streamflow and/or sediment supply and measurements of channel change are critical to many river management applications. Whereas repeated field surveys are costly and time‐consuming, remote sensing can be used to detect channel change at multiple temporal and spatial scales. Repeat images have been widely used to measure long‐term channel change, but these measurements are only significant if the magnitude of change exceeds the uncertainty. Existing methods for characterizing uncertainty have two important limitations. First, while the use of a spatially variable image co‐registration error avoids the assumption that errors are spatially uniform, this type of error, as originally formulated, can only be applied to linear channel adjustments, which provide less information on channel change than polygons of erosion and deposition. Second, previous methods use a level‐of‐detection (LoD) threshold to remove non‐significant measurements, which is problematic because real changes that occurred but were smaller than the LoD threshold would be removed. In this study, we present a new method of quantifying uncertainty associated with channel change based on probabilistic, spatially varying estimates of co‐registration error and digitization uncertainty that obviates a LoD threshold. The spatially distributed probabilistic (SDP) method can be applied to both linear channel adjustments and polygons of erosion and deposition, making this the first uncertainty method generalizable to all metrics of channel change. Using a case study from the Yampa River, Colorado, we show that the SDP method reduced the magnitude of uncertainty and enabled us to detect smaller channel changes as significant. Additionally, the distributional information provided by the SDP method allowed us to report the magnitude of channel change with an appropriate level of confidence in cases where a simple LoD approach yielded an indeterminate result

Open Heavy Flavor Production in QCD -- Conceptual Framework and Implementation Issues

Heavy flavor production is an important QCD process both in its own right and as a key component of precision global QCD analysis. Apparent disagreements between fixed-flavor scheme calculations of b-production rate with experimental measurements in hadro-, lepto-, and photo-production provide new impetus to a thorough examination of the theory and phenomenology of this process. We review existing methods of calculation, and place them in the context of the general PQCD framework of Collins. A distinction is drawn between scheme dependence and implementation issues related to quark mass effects near threshold. We point out a so far overlooked kinematic constraint on the threshold behavior, which greatly simplifies the variable flavor number scheme. It obviates the need for the elaborate existing prescriptions, and leads to robust predictions. It can facilitate the study of current issues on heavy flavor production as well as precision global QCD analysis.Comment: 13 pages, 10 figures, Proceedings of Ringberg Workshop: New Trends in HERA Physics 2001, Munich, German

Authors' Response

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