Evaluación de la respuesta productiva e inmune en juveniles de camarón Litopenaeus vannamei alimentado con mezclas probióticas

Shrimp diets with mixtures of probiotics (bacilli or yeasts) were evaluated on the production response and immunological effect on juvenile shrimp (Litopenaeus vannamei). The results showed a significant effect in growth, food consumption and feed conversion rate when probiotic diet was used. In addition, experimental diets with yeast mixture [C. insectorum (DH5), D. hansenii (DH6, and L1)] had a significantly higher amount of circulating haemocytes than those with bacilli [B. tequilensis (YC5-2), B. endophyticus (YC3-b) and B. endophyticus (C2-2)]. Shrimp fed both experimental diets (3x107 CFU mL-1) had a significantly higher growth and immune parameters when compared with the commercial diet. Both elements can contribute to the sustainable development and health of shrimp farming

Growth, survival, and superoxide dismutase activity in juvenile Crassostrea corteziensis (Hertlein, 1951) treated with probiotics

Juvenile seed of the Cortés oyster Crassostrea corteziensis were exposed to Lactobacillus sp. isolated from Nodipecten subnodosus, a mix of Pseudomonas sp. and Burkholderia cepacia, a marine yeast strain, a commercial probiotic (Epicin®), and oxytetracycline to determine their effect on growth, survival, SOD activity, and protein content. Probiotics at the test dose of 50,000 cells ml-1, Epicin and oxytetracycline at 7 mg l -1 were evaluated during 30 days of culture. Results showed that growth of C. corteziensis was significantly improved by Lactobacillus sp. and the bacilli mix significantly enhanced survival and SOD activity at the test dose. Protein content did not significantly increase by the treatments used. This study demonstrated the potential use of marine microbiota to improve cultivation of C. corteziensis

Dietary enrichment with crude protein content and feed additives (Bacillus spp. and yeast strains) improves growth performance, survival and circulating hemocytes in juvenile White shrimp, Litopenaeus vannamei: Enriquecimiento de la dieta con proteína y aditivos alimentarios (cepas de Bacillus spp. y levaduras) mejora el crecimiento, supervivencia y hemocitos circulantes de juveniles de camarón blanco, Litopenaeus vannamei

In this study the enrichment dietary effect with different  crude protein levels (CP) and feed additives on growth, survival, feed conversion ratio (FCR), feed consumption (FC) and total hemocyte count (THC) in juvenile white shrimp Litopenaeus vannamei were evaluated. The study covered two bioassays: in the first one, juveniles were daily fed for 45 days with four experimental diets containing: (1) Control, commercial feed (35% CP); (2) 29% CP; (3) 32% CP; (4) 35% CP. After the 29% CP diet was selected, juveniles in bioassay II were daily fed for 45 days with a single CP diet complemented with probiotics: (1) Control, commercial feed (35% CP); (2) 29% CP; (3) 29% CP + Bacillus mix at 1×106 CFUg–1 feed; and (4) 29% CP + yeast mix at 1×106 CFUg–1 feed. Juvenile shrimp fed with experimental diets gained significantly more weight and increased survival, FCR, FC and THC compared with control diet. However, differences among experimental diets were not significant. In bioassay II, juvenile shrimp fed with experimental diet + feed additives significantly increased survival, FCR, FC and THC compared with control diet. Growth of juveniles significantly increased with 29% CP and 29% CP + yeast mix diets, compared with control group. Complementing the diet with yeast mix showed higher survival and THC of juveniles compared with the other experimental treatments. Different CP levels in shrimp diet improved growth, survival and circulating hemocytes, and addition of mixed yeast as feed additive induced better survival and immune response in juvenile shrimp

Growth, survival, and superoxide dismutase activity in juvenile Crassostrea corteziensis (Hertlein, 1951) treated with probiotics

Juvenile seed of the Cortés oyster Crassostrea corteziensis were exposed to Lactobacillus sp. isolated from Nodipecten subnodosus, a mix of Pseudomonas sp. and Burkholderia cepacia, a marine yeast strain, a comercial probiotic (Epicin®), and oxytetracycline to determine their effect on growth, survival, SOD activity, and protein content. Probiotics at the test dose of 50,000 cells • ml-1, Epicin and oxytetracycline at 7 mg • l-1 were evaluated during 30 days of culture. Results showed that growth of C. corteziensis was significantly improved by Lactobacillus sp. and the bacilli mix significantly enhanced survival and SOD activity at the test dose. Protein content did not significantly increase by the treatments used. This study demonstrated the potential use of marine microbiota to improve cultivation of C. corteziensis

Superoxide dismutase activity in juvenile Litopenaeus vannamei and Nodipectensubnodosus exposed to the toxic dinoflagellate Prorocentrum lima

The toxic effect of the dinoflagellate Prorocentrum lima on juvenile American whiteleg shrimp Litopenaeus vannamei and giant lion-paw scallop Nodipecten subnodosus was evaluated. Organisms were exposed to three densities (500, 2000, or 5000 cells mL-1), superoxide dismutase activity and soluble protein in the hepatopancreas and muscle were determined at 1, 6, 24 and 48 h after challenge. Shrimp exposed at 5000 cells mL-1 significantly increased SOD activity in the hepatopancreas at 1 h post-challenge, whereas enzymatic activity in muscle significantly increased at 24 h at all densities. Scallops exposed to 500 and 2000 cells mL-1 showed significant SOD activity increase in hepatopancreas at 24 and 12 h, respectively. Mortality at 48 h was 100% in scallops exposed to 5000 cells mL-1. Shrimp showed higher levels of SOD activity than scallops. Soluble protein content in the shrimp hepatopancreas was significantly higher at densities of 500 and 2000 cells mL-1 at 6 and 1 h, respectively. Soluble protein content in the scallop hepatopancreas was higher than control values at 1 h after challenge. In this study, 500 cells mL-1 was enough to trigger SOD activity in two benthic species exposed to the toxic dinoflagellate P. lima