Locally convex structures on higher local fields

We establish how a higher local field can be described as a locally convex vector space once an embedding of a local field into it has been fixed. This extends previous results that had been obtained in the two-dimensional case. In particular, we study bounded and compactoid submodules of these fields and establish a self-duality result once a suitable topology on the dual space has been introduced.Comment: 18 page

Interaction of topology and algebra in arithmetic geometry

This thesis studies topological and algebraic aspects of higher dimensional local fields and relations to other neighbouring research areas such as nonarchimedean functional analysis and higher dimensional arithmetic geometry. We establish how a higher local field can be described as a locally convex space once an embedding of a local field into it has been fixed. We study the resulting spaces from a functional analytic point of view: in particular we introduce and study bounded, c-compact and compactoid submodules of characteristic zero higher local fields. We show how these spaces are isomorphic to their appropriately topologized duals and study the implications of this fact in terms of polarity. We develop a sequential-topological study of rational points of schemes of finite type over local rings typical in higher dimensional number theory and algebraic geometry. These rings are certain types of multidimensional complete fields and their rings of integers and include higher local fields. Our results extend the constructions of Weil over (one-dimensional) local fields. We establish the existence of an appropriate topology on the set of rational points of schemes of finite type over the rings considered, study the functoriality of this construction and deduce several properties

Attitudes of dental implantologists in Spain to prescribing antibiotics, analgesics and anti-inflammatories in healthy patients

The implantologists frequently prescribe antibiotics, analgesics and anti-inflammatories in dental implant surgery. The aims of this study were to evaluate the attitudes of implantologists in Murcia (Spain) to prescribing antibiotics, analgesics and anti-inflammatories in healthy patients during different implant dentistry procedures, and to see how these are influenced by individual dentist?s academic level, professional experience, and ongoing training (attending courses or reading scientific literature on medication use) This cross-sectional study included a total of 200 implantologists from the Murcia area (Spain), who each completed a two-page questionnaire consisting of 26 questions. The implant procedure in which most dentists (n=97) prescribed antibiotics was multiple implant surgery with flap raising, in which 55.6% of these 97 respondents used a prophylactic antibiotic regime for 7 days after implant placement. All subjects (n=200) prescribed analgesics for eight out of the eleven procedures included in the survey and anti-inflammatories in six. Dentists with higher academic levels or longer professional experience prescribed more antibiotics, but those who underwent continuous training (attending courses or reading scientific literature) reduced antibiotic prescription. Dentists often prescribed antibiotics, analgesics and anti-inflammatories in almost all implant procedures in healthy patients, but ongoing training reduced the frequency of antibiotic prescription in some procedures

Genetic characterization of maize landraces from Misiones, Argentina

En el norte de la Argentina habitan comunidades aborígenes Guaraníes que cultivan razas nativas de maíz. La caracterización genética del germoplasma autóctono, en particular de las razas de la Provincia de Misiones, es sumamente escasa. Con el fin de aportar al conocimiento de la diversidad genética y la estructura poblacional de estas razas nativas, contribuir a su conservación y evaluar su potencial como reservorio de nuevos alelos, se evaluaron 15 loci microsatélites nucleares en 467 individuos. Los resultados del presente estudio permiten concluir que: 1) Las razas de Misiones se encuentran constituidas por acervos génicos diferentes, que concuerdan con el tipo de grano que poseen, es decir, harinosos/córneo-harinosos y reventadores, grupos que deberían ser considerados como unidades de conservación diferentes; y 2) las razas nativas de Misiones constituyen una reserva de diversidad significativa, con potencial para ampliar la base genética de los programas de fitomejoramiento.Northern aboriginal communities from Argentina cultivate maize landraces for self-sufficiency. Genetic characterization of indigenous germplasm from Misiones Province is extremely limited. To contribute to the knowledge and conservation of the genetic diversity of these native maize landraces and to assess their potential as a reservoir of novel alleles, 467 individuals were evaluated using 15 nuclear microsatellite loci. The results of this study allow to conclude that: 1) landraces from Misiones can be divided into two different gene pools, which are consistent with the type of corn (flints and flours vs. popcorns), these should be regarded as different conservation units; and 2) Misiones landraces represent a significant reservoir of diversity, useful to broaden the genetic base of breeding programs.Fil: Bracco, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Lia, Veronica Viviana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poggio, Lidia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Camara Hernandez, Julian Alberto. Universidad de Buenos Aires. Facultad de Agronomia; ArgentinaFil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites

Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.Fil: Camara, Maria de Los Milagros. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Bouvier, Leon Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Reigada, Chantal. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentin

Molecular and functional characterization of a Trypanosoma cruzi nuclear adenylate kinase isoform

Trypanosoma cruzi, the etiological agent of Chagas’ disease, is an early divergent eukaryote in which control of gene expression relies mainly in post-transcriptional mechanisms. Transcription levels are globally up and down regulated during the transition between proliferating and non-proliferating life-cycle stages. In this work we characterized a nuclear adenylate kinase isoform (TcADKn) that is involved in ribosome biogenesis. Nuclear adenylate kinases have been recently described in a few organisms, being all related to RNA metabolism. Depending on active transcription and translation, TcADKn localizes in the nucleolus or the cytoplasm. A non-canonical nuclear localization signal was mapped towards the Nterminal of the protein, being the phosphate-binding loop essential for its localization. In addition, TcADKn nuclear exportation depends on the nuclear exportation adapter CRM1. TcADKn nuclear shuttling is governed by nutrient availability, oxidative stress and by the equivalent in T. cruzi of the mammalian TOR (Target of Rapamycin) pathway. One of the biological functions of TcADKn is ribosomal 18S RNA processing by direct interaction with ribosomal protein TcRps14. Finally, TcADKn expression is regulated by its 39 UTR mRNA. Depending on extracellular conditions, cells modulate protein translation rates regulating ribosome biogenesis and nuclear adenylate kinases are probably key components in these processes.Fil: Camara, Maria de Los Milagros. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;Fil: Bouvier, Leon Alberto. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;Fil: Canepa, Gaspar Exequiel. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;Fil: Miranda, Mariana Reneé. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina;Fil: Pereira, Claudio Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas "Dr. Alfredo Lanari"; Argentina

Efeito de diferentes formulações de glifosate no manejo de plantas daninhas na cultura do cafeeiro

An study was carried out in a commercial one-year-old coffee crop following undercutting, aiming to evaluate the efficiency of different glyphosate salts, that is, potassic, isopropylamine and diamonium in managing weeds in coffee tree crop. The treatments consisted of 10 different managements corresponding to three sequential applications of herbicides, that could be performed with or without the same products. The control efficiency of the weed species were verified at 15, 30, 41, 58, 75, 84, 116 and 140 days after beginning the management (DAM). The management with sequential application of 720 g.ha-1 ac.eq. of potassic salt glyphosate and diamonium salt glyphosate, as well as the management with diuron and paraquat (150 + 300 g.ha-1 a.i., respectively, with added Agral 0.1% v/v) at the third application or at the second and third applications, with the second application performed at 41 days after the first application, and the third one at 54 days after the second application, were all efficient in controlling Brachiaria plantaginea and Bidens pilosa, but inefficient in controlling Euphorbia heterophylla and Commelina diffusa. The management with 720 g.ha-1 ac.eq. of isopropylamine salt glyphosate at the first application, and latter at the second and third ones with the tank mixture of 720 g.ha-1 ac.eq. of isopropylamine salt glyphosate + 30 g.ha-1 a.i. of carfentrazone-ethyl, showed to be effective in controlling Brachiaria plantaginea and Bidens pilosa from 7 to 140 DAM and the Commelina diffusa from 58 to 140 DAM. However, Euphorbia heterophylla were moderately controlled over this period.Com o objetivo de avaliar a eficiência de diferentes sais de glifosate - potássico, isopropilamina e diamônio - no manejo de plantas daninhas na cultura do cafeeiro, foi realizado um experimento em lavoura comercial de café com um ano de idade, após a decepa. Os tratamentos foram constituídos por 10 diferentes manejos, correspondendo a três aplicações seqüenciais de herbicidas, que podiam ou não ser com os mesmos produtos. A eficiência de controle das espécies de plantas daninhas foi verificada aos 15, 30, 41, 58, 75, 84, 116 e 140 dias após o início dos manejos (DAM). Os manejos com aplicação seqüencial de 720 g.ha-1 de eq.ac. de glifosate sal potássico e diamônio, e os manejos com diuron e paraquat (150 + 300 g.ha-1 de i.a., respectivamente, e Agral a 0,1% v/v) na terceira aplicação, ou na segunda e na terceira aplicação, com a segunda realizada aos 41 dias após a primeira e a terceira aos 54 dias após a segunda aplicação, foram eficientes no controle de Brachiaria plantaginea e Bidens pilosa e ineficientes no manejo de Euphorbia heterophylla e Commelina diffusa. O manejo com 720 g.ha-1 de eq.ac. de glifosate sal isopropilamina na primeira aplicação e posteriormente na segunda e terceira aplicações, com a mistura no tanque de 720 g.ha-1 de eq.ac. de glifosate + 30 g. ha-1 de i.a. de carfentrazone-ethyl, foi eficaz no controle de Brachiaria plantaginea e Bidens pilosa dos 7 aos 140 DAM e Commelina diffusa dos 58 aos 140 DAM. Todavia, Euphorbia heterophylla demonstrou controle moderado neste período