PS-HEMA latex fractionation by sedimentation and colloidal crystallization

A poly(styrene-co-hydroxyethylmethacrylate) latex underwent sedimentation under gravity followed by an spontaneous and extensive colloidal crystallization. It was then fractionated in three visually distinguishable layers. Latex aliquots layers were sampled at different heigths and the particles were characterized by PCS, microelectrophoresis, infrared spectra and analytical electron microscopy. The major fraction was opalescent and contained the colloidal crystals settled in the bottom of the liquid. Two other latex fractions were obtained, which differed in their chemical compositions, particle sizes and topochemical features from the self-arraying particles. Macrocrystallization of the fractionated latex yielded high quality crystals with a low frequency of defects, which confirms that particle chemical homogeneity is an important factor for particle self-arraying.Látex de poli(estireno-co-hidroxiacrilato de metila) separa-se em três camadas visualmente distinguíveis, das quais a inferior é opalescente e contém cristais coloidais. Alíquotas do látex foram coletadas em diferentes alturas, e as partículas foram caracterizadas, por espalhamento de luz dinâmico, microeletroforese, IV e microscopia eletrônica analítica. A fração inferior contém a maior parte do polímero, sendo formada por partículas de dimensões e composição química uniformes. As partículas coletadas das duas outras frações são diferentes das que formam os cristais coloidais, em praticamente todos os aspectos. A secagem da fração opalescente produz macrocristais de alta qualidade, com baixa frequência de defeitos, mostrando que a homogeneidade química das partículas é um fator importante, na sua auto-organização.497504Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Por um sistema de inovação organizacional

Dizer que o grande segredo do sucesso das empresas, especialmente em tempos conturbados, é a sua adaptabilidade às condições do mercado, parece um lugar comum mas não deixa de ser verdade. No entanto, afirmar que a adaptabilidade é função da capacidade das chefias em levar os colaboradores a pensar em modos inovadores, já não parece tão evidente, por isso iremos dedicar este artigo à demonstração desta perspectiva

Effect of Process Parameters on Bioelectricity Production, Energy and Environmental Performance

This study evaluates the energy and environmental performance of a cogeneration system operating simultaneously with bagasse and straw in a rankine cycle. Different process conditions, defined in terms of boiler operating pressure, moisture content and straw addition rate, were analyzed. The combination of these parameters led to the elaboration of one hundred and twenty-five analysis scenarios. The energy profile was based on the energy performance indicator, which is the ratio between the exported electricity and the intrinsic energy of biomass consumed for its generation, and the environmental assessment was performed in terms of climate change. Scenario modeling was developed according to the conceptual framework proposed by the life cycle assessment technique with a ‘from cradle-to-gate’ coverage. Results indicate that the best energy and environmental performances (energy performance indicator = 0.193 and 688 kg CO2eq/MWh) were obtained with the highest pressure (100 bar) and addition rate (50%), and the lowest moisture content (10%). Moreover, straw moisture has more influence on the system environmental performance than its addition rate

Monoclonal antibody to Olive latent virus 1

Olive is a crop of high economic importance in Portugal. Its susceptibility to virus infections justifies research leading to accurate virus detection. Two necroviruses mechanically transmissible to herbaceous indicator plants have been isolated from symptomatic olive trees: Olive latent virus 1 (OLV-1) and Tobacco necrosis virus D (TNV-D). OLV-1 was also isolated from citrus trees in Turkey and from tulips in Japan. In this study, we attempted to prepare a monoclonal antibody (MAb) specific to OLV-1, which could be used in ELISA for accurate diagnostics of this virus

Isolation of an acetyl-CoA synthetase gene (ZbACS2) from Zygosaccharomyces bailii

A gene homologous to Saccharomyces cerevisiae ACS genes, coding for acetyl-CoA synthetase, has been cloned from the yeast Zygosaccharomyces bailii ISA 1307, by using reverse genetic approaches. A probe obtained by PCR amplification from Z. bailii DNA, using primers derived from two conserved regions of yeast ACS proteins, RIGAIHSVVF (ScAcs1p; 210–219) and RVDDVVNVSG (ScAcs1p; 574–583), was used for screening a Z. bailii genomic library. Nine clones with partially overlapping inserts were isolated. The sequenced DNA fragment contains a complete ORF of 2027 bp (ZbACS2) and the deduced polypeptide shares significant homologies with the products of ACS2 genes from S. cerevisiae and Kluyveromyces lactis (81% and 82% identity and 84% and 89% similarity, respectively). Phylogenetic analysis shows that the sequence of Zbacs2 is more closely related to the sequences from Acs2 than to those from Acs1 proteins. Moreover, this analysis revealed that the gene duplication producing Acs1 and Acs2 proteins has occurred in the common ancestor of S. cerevisiae, K. lactis, Candida albicans, C. glabrata and Debaryomyces hansenii lineages. Additionally, the cloned gene allowed growth of S. cerevisiae Scacs2 null mutant, in medium containing glucose as the only carbon and energy source, indicating that it encodes a functional acetyl-CoA synthetase. Also, S. cerevisiae cells expressing ZbACS2 have a shorter lag time, in medium containing glucose (2%, w/v) plus acetic acid (0.1–0.35%, v/v). No differences in cell response to acetic acid stress were detected both by specific growth and death rates. The mode of regulation of ZbACS2 appears to be different from ScACS2 and KlACS2, being subject to repression by a glucose pulse in acetic acid-grown cells. The nucleotide sequence of a common 5269 bp fragment has been deposited in the EMBL Data Library under Accession No. AJ314837.Fundação para a Ciência e a Tecnologia (FCT) - PRAXIS XXI P/AGR/11135/9

Absorption and quasinormal modes of classical fields propagating on 3D and 4D de Sitter spacetime

We extensively study the exact solutions of the massless Dirac equation in 3D de Sitter spacetime that we published recently. Using the Newman-Penrose formalism, we find exact solutions of the equations of motion for the massless classical fields of spin s=1/2,1,2 and to the massive Dirac equation in 4D de Sitter metric. Employing these solutions, we analyze the absorption by the cosmological horizon and de Sitter quasinormal modes. We also comment on the results given by other authors.Comment: 31 page

Stability and quasi-normal modes of charged black holes in Born-Infeld gravity

In this paper we study the stability and quasi-normal modes of scalar perturbations of black holes. The static charged black hole considered here is a solution to Born-Infeld electrodynamics coupled to gravity. We conclude that the black hole is stable. We also compare the stability of it with its linear counter-part Reissner-Nordstrom black hole. The quasi-normal modes are computed using the WKB method. The behavior of these modes with the non-linear parameter, temperature, mass of the scalar field and the spherical index are analyzed in detail.Comment: Latex, 17 pages, 13 figures, some sections edited, references adde

The Saffman-Taylor problem on a sphere

The Saffman-Taylor problem addresses the morphological instability of an interface separating two immiscible, viscous fluids when they move in a narrow gap between two flat parallel plates (Hele-Shaw cell). In this work, we extend the classic Saffman-Taylor situation, by considering the flow between two curved, closely spaced, concentric spheres (spherical Hele-Shaw cell). We derive the mode-coupling differential equation for the interface perturbation amplitudes and study both linear and nonlinear flow regimes. The effect of the spherical cell (positive) spatial curvature on the shape of the interfacial patterns is investigated. We show that stability properties of the fluid-fluid interface are sensitive to the curvature of the surface. In particular, it is found that positive spatial curvature inhibits finger tip-splitting. Hele-Shaw flow on weakly negative, curved surfaces is briefly discussed.Comment: 26 pages, 4 figures, RevTex, accepted for publication in Phys. Rev.

The Highly Damped Quasinormal Modes of dd-dimensional Reissner-Nordstrom Black Holes in the Small Charge Limit

We analyze in detail the highly damped quasinormal modes of $d$-dimensional Reissner-Nordstr$\ddot{\rm{o}}$m black holes with small charge, paying particular attention to the large but finite damping limit in which the Schwarzschild results should be valid. In the infinite damping limit, we confirm using different methods the results obtained previously in the literature for higher dimensional Reissner-Nordstr$\ddot{\rm{o}}$m black holes. Using a combination of analytic and numerical techniques we also calculate the transition of the real part of the quasinormal mode frequency from the Reissner-Nordstr$\ddot{\rm{o}}$m value for very large damping to the Schwarzschild value of $\ln(3) T_{bh}$ for intermediate damping. The real frequency does not interpolate smoothly between the two values. Instead there is a critical value of the damping at which the topology of the Stokes/anti-Stokes lines change, and the real part of the quasinormal mode frequency dips to zero.Comment: 18 pages, 8 figure

Intermediate Asymptotics of the Kerr Quasinormal Spectrum

We study analytically the quasinormal mode spectrum of near-extremal (rotating) Kerr black holes. We find an analytic expression for these black-hole resonances in terms of the black-hole physical parameters: its Bekenstein-Hawking temperature T_{BH} and its horizon's angular velocity \Omega, which is valid in the intermediate asymptotic regime 1<<\omega<<1/T_{BH}.Comment: 4 page