309 research outputs found
Comparison of two competitive ELISAs for the detection of specific peste-des-petits-ruminant antibodies in sheep and cattle populations
Slaughterhouse zoonoses: Are workers reservoirs of zoonotic disease?
Slaughterhouse workers are considered a high risk group for zoonotic disease due to increased contact
with animals, animal products and excreta. Globally, slaughterhouse workers have been shown to have an
increased seroprevalence to zoonotic pathogens, though no such studies exist in Kenya. Slaughterhouse
workers may also act as reservoirs of these zoonotic organisms and asymptomatic carriage of pathogenic
bacteria has been demonstrated. This study aimed to determine the carriage of enteropathogens in
slaughterhouse workers in rural western Kenya and to determine if there was asymptomatic carriage of
Staphylococcus aureus specifically Methicillin Resistant S. aureus (MRSA). This study was conducted
in the Lake Victoria Crescent region of western Kenya. Five hundred slaughterhouse workers from this
region were asked a comprehensive questionnaire regarding risk factors for zoonotic disease, faecal samples
were collected for examination and culture and a nasal swab was cultured for S. aureus. This study reports
on the seroprevalence of Salmonella spp, Shigella spp, pathogenic Escherichia coli and Campylobacter
spp. in these individuals as well as reporting the nasal carriage of S. aureus and MRSA. This is the first
community based study regarding S. aureus and MRSA in Kenya. The asymptomatic carriage of these
organisms in slaughterhouse workers highlights a reservoir that may be important in the dissemination of
these pathogens. The study further comments on the risk factors for infection with these pathogens and
suggestions are made for simple hygiene interventions that can reduce disease in slaughterhouse workers
and dissemination to the wider communit
Redefining the "carrier" state for foot-and-mouth disease from the dynamics of virus persistence in endemically affected cattle populations
The foot-and-mouth disease virus (FMDV) âcarrierâ state was defined by van Bekkum in 1959. It was based on the recovery of infectious virus 28 days or more post infection and has been a useful construct for experimental studies. Using historic data from 1,107 cattle, collected as part of a population based study of endemic FMD in 2000, we developed a mixed effects logistic regression model to predict the probability of recovering viable FMDV by probang and culture, conditional on the animalâs age and time since last reported outbreak. We constructed a second set of models to predict the probability of an animal being probang positive given its antibody response in three common non-structural protein (NSP) ELISAs and its age. We argue that, in natural ecological settings, the current definition of a âcarrierâ fails to capture the dynamics of either persistence of the virus (as measured by recovery using probangs) or the uncertainty in transmission from such animals that the term implies. In these respects it is not particularly useful. We therefore propose the first predictive statistical models for identifying persistently infected cattle in an endemic setting that captures some of the dynamics of the probability of persistence. Furthermore, we provide a set of predictive tools to use alongside NSP ELISAs to help target persistently infected cattle
Repeated high doses of avermectins cause prolonged sterilisation, but do not kill, Onchocerca ochengi adult worms in African cattle
BACKGROUND: Ivermectin (Mectizanâą, Merck and CO. Inc.) is being widely used in the control of human onchocerciasis (Onchoverca volvulus) because of its potent effect on microfilariae. Human studies have suggested that, at the standard dose of 150 ÎŒg/kg an annual treatment schedule of ivermectin reversibly interferes with female worm fertility but is not macrofilaricidal. Because of the importance of determining whether ivermectin could be macrofilaricidal, the efficacy of high and prolonged doses of ivermectin and a related avermectin, doramectin, were investigated in cattle infected with O. ochengi. METHODS: Drugs with potential macrofilaricidal activity, were screened for the treatment of human onchocerciasis, using natural infections of O. ochengi in African cattle. Three groups of 3 cows were either treated at monthly intervals (7 treatments) with ivermectin (Ivomec(Âź), Merck and Co. Inc.) at 500 ÎŒg/kg or doramectin (Dectamax(Âź), Pfizer) at 500 ÎŒg/kg or not treated as controls. Intradermal nodules were removed at 6 monthly intervals and adult worms were examined for signs of drug activity. RESULTS: There was no significant decline in nodule diameter, the motility of male and female worms, nor in male and female viability as determined by the ability to reduce tetrazolium, compared with controls, at any time up to 24 months from the start of treatments (mpt). Embryogenesis, however, was abrogated by treatment, which was seen as an accumulation of dead and dying intra-uterine microfilariae (mf) persisting for up to 18 mpt. Skin mf densities in treated animals had fallen to zero by <3 mpt, but by 18 mpt small numbers of mf were found in the skin of some treated animals and a few female worms were starting to produce multi-cellular embryonic stages. Follow-up of the doramectin treated group at 36 mpt showed that mf densities had still only regained a small proportion of their pre-treatment levels. CONCLUSION: These results have important implications for onchocerciasis control in the field. They suggest that ivermectin given at repeated high does may sterilise O. volvulus female worms for prolonged periods but is unlikely to kill them. This supports the view that control programmes may need to continue treatments with ivermectin for a period of decades and highlights the need to urgently identify new marcofiliaricidal compounds
Serological Patterns of Brucellosis, Leptospirosis and Q Fever in Bos indicus Cattle in Cameroon
Brucellosis, leptospirosis and Q fever are important infections of livestock causing a range of clinical conditions including abortions and reduced fertility. In addition, they are all important zoonotic infections infecting those who work with livestock and those who consume livestock related products such as milk, producing non-specific symptoms including fever, that are often misdiagnosed and that can lead to severe chronic disease. This study used banked sera from the Adamawa Region of Cameroon to investigate the seroprevalences and distributions of seropositive animals and herds. A classical statistical and a multi-level prevalence modelling approach were compared. The unbiased estimates were 20% of herds were seropositive for Brucella spp. compared to 95% for Leptospira spp. and 68% for Q fever. The within-herd seroprevalences were 16%, 35% and 39% respectively. There was statistical evidence of clustering of seropositive brucellosis and Q fever herds. The modelling approach has the major advantage that estimates of seroprevalence can be adjusted for the sensitivity and specificity of the diagnostic test used and the multi-level structure of the sampling. The study found a low seroprevalence of brucellosis in the Adamawa Region compared to a high proportion of leptospirosis and Q fever seropositive herds. This represents a high risk to the human population as well as potentially having a major impact on animal health and productivity in the region
A scoping review of foot-and-mouth disease risk, based on spatial and spatio-temporal analysis of outbreaks in endemic settings
International audienc
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