Modelli di integrazione di reti bayesiane ed ontologie: analisi e confronto

Le ontologie sono lo standard de facto per la rappresentazione di conoscenza su concetti e le relazioni che li legano in specifici domini. Tuttavia, le ontologie non possono rappresentare la conoscenza parziale o incompleta che esiste nei domini di applicazione. C'è un interesse crescente nell'integrazione di tecniche di ragionamento probabilistico nei sistemi di inferenza delle ontologie. Questa tesi propone un'analisi ed il confronto dello stato dell'arte nell'integrazione fra reti bayesiane e ontologie

An analytical description of the energy balance in turbulent, round, free jets

This brief contribution provides a quantification of the terms of the turbulent kinetic energy transport equation for a round steady turbulent free jet. The analysis is based on the assumption of flow self-similarity, and it is performed by means of a simple analytical asymptotic analysis. The results are in good agreement with the experimental findings of Panchapakesan and Lumley [J. Fluid Mech. 246, 197–223 (1993)] and with the large eddy simulations of Bogey and Bailly [J. Fluid Mech. 627, 129–160 (2009)], hence providing a theoretical interpretation of such findings

Noncovalent complexation of amphotericin-B with Poly(α-glutamic acid).

A noncovalent complex of amphotericin B (AmB) and poly(α-glutamic acid) (PGA) was prepared to develop a safe and stable formulation for the treatment of leishmaniasis. The loading of AmB in the complex was in the range of ∼20-50%. AmB was in a highly aggregated state with an aggregation ratio often above 2.0. This complex (AmB-PGA) was shown to be stable and to have reduced toxicity to human red blood cells and KB cells compared to the parent compound; cell viability was not affected at an AmB concentration as high as 50 and 200 μg/mL respectively. This AmB-PGA complex retained AmB activity against intracellular Leishmania major amastigotes in the differentiated THP-1 cells with an EC50 of 0.07 ± 0.03-0.08 ± 0.01 μg/mL, which is similar to Fungizone (EC50 of 0.06 ± 0.01 μg/mL). The in vitro antileishmanial activity of the complex against Leishmania donovani was retained after storage at 37 °C for 7 days in the form of a solution (EC50 of 0.27 ± 0.03 to 0.35 ± 0.04 μg/mL) and for 30 days as a solid (EC50 of 0.41 ± 0.07 to 0.63 ± 0.25 μg/mL). These encouraging results indicate that the AmB-PGA complex has the potential for further development

Fc-fusion mimetics

The Fc-fusion mimetic RpR 2[combining low line] was prepared by disulfide bridging conjugation using PEG in the place of the Fc. RpR 2[combining low line] displayed higher affinity for VEGF than aflibercept. This is caused primarily by a slower dissociation rate, which can prolong a drug at its site of action. RpRs have considerable potential for development as stable, organ specific therapeutics

A simple route to functionalising electrospun polymer scaffolds with surface biomolecules

Surface functionalisation of polymeric electrospun scaffolds with therapeutic biomolecules is often explored in regenerative medicine and tissue engineering. However, the bioconjugation method must be carefully selected to prevent partial or full loss of activity of the biomolecule following chemical manipulation. Perfluorophenyl azide bearing a N‐hydroxysuccinimide (PFPA-NHS) active ester group is a versatile tool for UV-initiated covalent coupling of amine-containing molecules to hydrocarbon- based polymers, such as polydioxanone or polycaprolactone (PCL). This study therefore explored the feasibility of PFPA-NHS functionalisation of electrospun PCL scaffolds with model biomolecules. Protein conjugation was extensively explored using fluorescence staining and attachment studies, confirming the retention of amine coupling capability following photografting of PFPA-NHS to the PCL surface. The effect of the washing method used to remove unreacted PFPA was explored in Caco-2 cell viability studies, and it was determined that sonication washing is required to avoid cell death. A model enzyme, catalase, was then successfully attached to the surface of PCL scaffolds for potential applications in oncological photodynamic therapy. Catalase retained its enzymatic activity following attachment to the fibres and the majority of the enzyme (∼60%) remained bound to the fibre after incubation in an aqueous environment for six days. The anticipated prolonged presentation and sustained release of proteins as a result of PFPA-NHS conjugation could be advantageous in progressing protein-based therapies

Measuring antibody coatings on gold nanoparticles by optical spectroscopy

The adsorption of antibodies onto gold nanoparticles to make gold–antibody conjugates is finding application in multiple areas. Gold–antibody conjugates for use in malaria diagnostics were prepared and a method of characterisation that can be applied to any gold–protein conjugate was developed. When protein adsorbs onto a gold nanoparticle, it changes the local refractive index and so changes the surface plasmon resonance of the gold particle. Changes to the surface plasmon resonance manifest in the absorbance spectrum of the conjugates. This was measured by optical spectroscopy and relatively simple equations to convert spectral shifts to predictions of the protein layer thickness and mass coverage are presented. As with most protein adsorption reported in the literature, the results showed the protein adsorption to depend on antibody concentration, reaching a plateau at around 1 μg ml−1. The coverage was estimated to be approximately 2–3 mg m−2 and the coating thickness estimates were approximately 10 nm, which is consistent with active antibody. The results suggested more antibody was used in conjugate preparation than was necessary for complete coverage of the gold. This excess antibody could bind to the target antigen to reduce malaria test sensitivity. A key advantage of this characterisation method is that it is sufficiently simple to be used for quality control of conjugate production and the equations presented can be applied to other coatings on gold nanoparticles

Gli effetti dell''uninominale secca' sul sistema partitico e sulla stabilità dei governi. Un'analisi comparata a largo raggio

Maurice Duverger formulò negli anni Cinquanta le note tesi sulla relazione tra i sistemi elettorali e i sistemi di partito. Se è vero che il sistema elettorale non è certamente l'unica variabile che condiziona il sistema partitico, la sua influenza è ineludibile in un regime democratico. L'obbiettivo di questo lavoro è verificare la fondatezza della tesi di Duverger che sosteneva l'esistenza di una relazione, considerata alla stessa stregua di una legge sociologica, tra sistemi elettorali maggioritari ad un turno – cioè con formula plurality applicata in collegi uninominali, quella che nel linguaggio corrente viene in Italia chiamata «uninominale secca» – e formato bipartitico