The recovery of asteroids after two observations

It is shown that a generalization of the use of 'Vaisala orbits', briefly mentioned at the Asteroids 2 Conference, can be very conveniently accomplished by means of an inversion of the 'GEM' form of the Gauss method. The procedure can also be applied to Apollo objects and to indeterminate cases of normal three-observation orbit computation, and there is also a simple extension to situations involving four or more observations

Ion exchange in porous crystals

Imperial Users onl

Astrometric observations of comets and asteroids and subsequent orbital investigations

The 155-cm reflector was used for observations of comets and minor planets on 28 nights during April-October. Twenty-two of the observations refer to comets, 62 to numbered minor planets (numbered, that is, by the end of the semester: only nine of them refer to minor planets already numbered at the time of the last report), and the remainer to unnumbered minor planets. Observations were made of four new comets discovered during the semester and a fifth discovered in January. Observations of Wilson (19861), P/Tempel 2 and two other returning short-period comets are also continuing. Among the odd numbered minor planets observed were the earth-approaching objects (1685) Toro and (1980) Tezcatlipoca. The Earth approacher 1980 PA was numbered (3908)

Astrometric observations of comets and asteroids and subsequent orbital investigations

The 155-cm reflector of the Smithsonian Astrophysical Observatory was used for observations of comets and minor planets on 35 nights during October l986- April l987. The distrubution was fairly uniform November-February, but poor weather permitted only 21 observations during March. Table I lists the 423 measurements published (in the MPCs) since the last report, two of them actually made at the very beginning of May l987. Forty-six of the observations refer to comets, 193 to numbered minor planets (numbered, that is, by the end of the semester; only 11 of them refer to minor planets already numbered at the time of the last report), and the remainder to unnumbered minor planets

N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools

Soluble protein expression is a key requirement for biochemical and structural biology approaches to study biological systems in vitro. Production of sufficient quantities may not always be achievable if proteins are poorly soluble which is frequently determined by physico-chemical parameters such as intrinsic disorder. It is well known that discrete protein domains often have a greater likelihood of high-level soluble expression and crystallizability. Determination of such protein domain boundaries can be challenging for novel proteins. Here, we outline the application of bioinformatics tools to facilitate the prediction of potential protein domain boundaries, which can then be used in designing expression construct boundaries for parallelized screening in a range of heterologous expression systems

Synthesis of Glycosidase Substrates Containing Self-Immolative Linkers

Glycosidases are enzymes responsible for the hydrolysis of glycosidic bonds in carbohydrates. The vital role they play in various biological processes makes them an important area of research. This project focuses on the synthesis of specific substrates that may be used to analyze the activity of glycosidases. Previous research in this area has used substrates composed of sugar molecules bonded to a fluorophore indicator to analyze enzyme activity. However, these substrates sometimes lack the selectivity required to discern between different enzymes that act on similar carbohydrates. Our method will preferentially measure the activity of specific glycosidases by using self-immolative linker molecules to achieve selectivity. The self-immolative linkers will act as a bridge between the sugar and fluorophore, but will spontaneously disintegrate following the hydrolysis of the bond to the sugar. sThis will release the fluorophore and allow for the enzyme’s activity to be measured. Introducing the linkers allows for greater variation in the interactions between the enzyme and substrate. These interactions include, but are not limited to, steric effects, hydrogen bonding, and van der Waals contacts. It is hoped that the linkers will interact differently with the various glycosidases and provide the desired selectivity. A library of compounds with various linkers has been synthesized using two different sugars, glucose and galactose. The progress on the creation of the sugar-linker library will be reported. *Indicates faculty mento

Astrometric Observations of Comets and Asteroids and Subsequent Orbital Investigations

An earlier series of photographic observations was made with the 1.5-m reflector from 1972 to 1989. The start of the series to which this report refers occurred shortly before the conversion from photographic to CCD operation in August 1989, at which point there was a dramatic increase in the productivity of the program. This is evident gives a month-by-month summary of the observations; the earlier data refer to the measurement or remeasurement of photographic plates previously taken with the same telescope. The total number of observations made was 24,423, of which 1338 were of comets. Of the 23,085 observations of asteroids, 21,529 referred to asteroids that were unnumbered when the observations were made. Since an important emphasis of the program was to improve knowledge of the orbits to the point where asteroids can be numbered, the fact that only 4262 of the observations refer to asteroids that are still unnumbered is a measure of the program's success, with 30-35 percent of all the new numberings being habitually made solely because of the recent data from the Oak Ridge program, which even at the time of McCrosky's retirement was still the fourth largest comet-asteroid astrometric program in the world

Characterization of designed, synthetically accessible bryostatin analog HIV latency reversing agents.

HIV latency in resting CD4+ T cell represents a key barrier preventing cure of the infection with antiretroviral drugs alone. Latency reversing agents (LRAs) can activate HIV expression in latently infected cells, potentially leading to their elimination through virus-mediated cytopathic effects, host immune responses, and/or therapeutic strategies targeting cells actively expressing virus. We have recently described several structurally simplified analogs of the PKC modulator LRA bryostatin (termed bryologs) designed to improve synthetic accessibility, tolerability in vivo, and efficacy in inducing HIV latency reversal. Here we report the comparative performance of lead bryologs, including their effects in reducing cell surface expression of HIV entry receptors, inducing proinflammatory cytokines, inhibiting short-term HIV replication, and synergizing with histone deacetylase inhibitors to reverse HIV latency. These data provide unique insights into structure-function relationships between A- and B-ring bryolog modifications and activities in primary cells, and suggest that bryologs represent promising leads for preclinical advancement