Evolutionary and pulsation properties of Type II Cepheids

We discuss the observed pulsation properties of Type II Cepheids (TIICs) in the Galaxy and Magellanic Clouds. The period (P) distributions, luminosity amplitudes and population ratios of the three different sub-groups (BL Herculis[BLH, P20 days]) are quite similar in different stellar systems, suggesting a common evolutionary channel and a mild dependence on both metallicity and environment. We present a homogeneous theoretical framework based on Horizontal Branch (HB) evolutionary models, envisaging that TIICs are mainly old (t<10 Gyr), low-mass stars. The BLHs are predicted to be post early asymptotic giant branch (PEAGB) stars (double shell burning) on the verge of reaching their AGB track (first crossing of the instability strip), while WVs are a mix of PEAGB and post-AGB stars (hydrogen shell burning) moving from cool to hot (second crossing). Thus suggesting that they are a single group of variable stars. RVTs are predicted to be a mix of post-AGB stars along their second crossing (short-period tail) and thermally pulsing AGB stars (long-period tail) evolving towards their white dwarf cooling sequence. We also present several sets of synthetic HB models by assuming a bimodal mass distribution along the HB. Theory suggests, in agreement with observations, that TIIC pulsation properties marginally depend on metallicity. Predicted period distributions and population ratios for BLHs agree quite well with observations, while those for WVs and RVTs are almost a factor of two smaller and larger than observed, respectively. Moreover, the predicted period distributions for WVs peak at periods shorter than observed, while those for RVTs display a long period tail not supported by observations. We investigate several avenues to explain these differences, but more detailed calculations are required to address them

On the separation between RR Lyrae and Type II Cepheids and their importance for distance determination: the case of ωω Cen

The separation between RR Lyrae (RRLs) and Type II Cepheid (T2Cs) variables based on their period is debated. Both types of variable stars are distance indicators and we aim to promote the use of T2Cs as distance indicators in synergy with RRLs. We adopted new and existing optical and Near-Infrared (NIR) photometry of \wcen~to investigate several diagnostics (colour-magnitude diagram, Bailey diagram, Fourier decomposition of the light curve, amplitude ratios) for their empirical separation. We found that the classical period threshold at 1 day is not universal and does not dictate the evolutionary stage: V92 has a period of 1.3 days but is likely to be still in its core Helium-burning phase, typical of RRLs. We also derived NIR Period-Luminosity relations and found a distance modulus of 13.65$\pm$0.07 (err.)$\pm$0.01 ($\sigma$) mag, in agreement with the recent literature. We also found that RRLs and T2Cs obey the same PL relations in the NIR. This equivalence gives the opportunity to adopt RRLs+T2Cs as an alternative to classical Cepheids to calibrate the extragalactic distance scale

Decitabine impact on the endocytosis regulator RhoA, the folate carriers RFC1 and FOLR1, and the glucose transporter GLUT4 in human tumors.

BackgroundIn 31 solid tumor patients treated with the demethylating agent decitabine, we performed tumor biopsies before and after the first cycle of decitabine and used immunohistochemistry (IHC) to assess whether decitabine increased expression of various membrane transporters. Resistance to chemotherapy may arise due to promoter methylation/downregulation of expression of transporters required for drug uptake, and decitabine can reverse resistance in vitro. The endocytosis regulator RhoA, the folate carriers FOLR1 and RFC1, and the glucose transporter GLUT4 were assessed.ResultsPre-decitabine RhoA was higher in patients who had received their last therapy &gt;3&nbsp;months previously than in patients with more recent prior therapy (P = 0.02), and varied inversely with global DNA methylation as assessed by LINE1 methylation (r = -0.58, P = 0.006). Tumor RhoA scores increased with decitabine (P = 0.03), and RFC1 also increased in patients with pre-decitabine scores ≤150 (P = 0.004). Change in LINE1 methylation with decitabine did not correlate significantly with change in IHC scores for any transporter assessed. We also assessed methylation of the RFC1 gene (alias SLC19A1). SLC19A1 methylation correlated with tumor LINE1 methylation (r = 0.45, P = 0.02). There was a small (statistically insignificant) decrease in SLC19A1 methylation with decitabine, and there was a trend towards change in SLC19A1 methylation with decitabine correlating with change in LINE1 methylation (r = 0.47, P &lt;0.15). While SLC19A1 methylation did not correlate with RFC1 scores, there was a trend towards an inverse correlation between change in SLC19A1 methylation and change in RFC1 expression (r = -0.45, P = 0.19).ConclusionsIn conclusion, after decitabine administration, there was increased expression of some (but not other) transporters that may play a role in chemotherapy uptake. Larger patient numbers will be needed to define the extent to which this increased expression is associated with changes in DNA methylation

On the Dwarf Irregular Galaxy NGC 6822. I. Young, Intermediate, and Old Stellar Populations

We present accurate and deep multiband (g, r, i) photometry of the Local Group dwarf irregular galaxy NGC 6822. The images were collected with wide-field cameras at 2 m/4 m (INT, CTIO, CFHT) and 8 m class telescopes (Subaru) covering a 2 deg2 field of view across the center of the galaxy. We performed point-spread function photometry of ≈7000 CCD images, and the final catalog includes more than 1 million objects. We developed a new approach to identify candidate field and galaxy stars and performed a new estimate of the galaxy center by using old stellar tracers, finding that it differs by 1.′15 (R.A.) and 1.′53 (decl.) from previous estimates. We also found that young (main sequence, red supergiants), intermediate (red clump, asymptotic giant branch (AGB)), and old (red giant branch) stars display different radial distributions. The old stellar population is spherically distributed and extends to radial distances larger than previously estimated (∼1°). The young population shows a well-defined bar and a disk-like distribution, as suggested by radio measurements, that is off-center compared with the old population. We discuss pros and cons of the different diagnostics adopted to identify AGB stars and develop new ones based on optical-near-IR-mid-IR color-color diagrams to characterize oxygen- and carbon-rich stars. We found a mean population ratio between carbon and M-type (C/M) stars of 0.67 ± 0.08 (optical/near-IR/mid-IR), and we used the observed C/M ratio with empirical C/M-metallicity relations to estimate a mean iron abundance of [Fe/H] ∼-1.25 (σ = 0.04 dex), which agrees quite well with literature estimates

Alternative Complement Pathway Deregulation Is Correlated with Dengue Severity

BACKGROUND:The complement system, a key component that links the innate and adaptive immune responses, has three pathways: the classical, lectin, and alternative pathways. In the present study, we have analyzed the levels of various complement components in blood samples from dengue fever (DF) and dengue hemorrhagic fever (DHF) patients and found that the level of complement activation is associated with disease severity. METHODS AND RESULTS:Patients with DHF had lower levels of complement factor 3 (C3; p = 0.002) and increased levels of C3a, C4a and C5a (p<0.0001) when compared to those with the less severe form, DF. There were no significant differences between DF and DHF patients in the levels of C1q, immunocomplexes (CIC-CIq) and CRP. However, small but statistically significant differences were detected in the levels of MBL. In contrast, the levels of two regulatory proteins of the alternative pathway varied widely between DF and DHF patients: DHF patients had higher levels of factor D (p = 0.01), which cleaves factor B to yield the active (C3bBb) C3 convertase, and lower levels of factor H (p = 0.03), which inactivates the (C3bBb) C3 convertase, than did DF patients. When we considered the levels of factors D and H together as an indicator of (C3bBb) C3 convertase regulation, we found that the plasma levels of these regulatory proteins in DHF patients favored the formation of the (C3bBb) C3 convertase, whereas its formation was inhibited in DF patients (p<0.0001). CONCLUSION:The data suggest that an imbalance in the levels of regulatory factors D and H is associated with an abnormal regulation of complement activity in DHF patients