Migratory myiasis in a European traveller due to Hypoderma larvae

Immunogenetics and cellular immunology of bacterial infectious disease

A highly polymorphic effector protein promotes fungal virulence through suppression of plant-associated Actinobacteria

Plant pathogens secrete effector proteins to support host colonization through a wide range of molecular mechanisms, while plant immune systems evolved receptors to recognize effectors or their activities to mount immune responses to halt pathogens. Importantly, plants do not act as single organisms, but rather as holobionts that actively shape their microbiota as a determinant of health. The soil-borne fungal pathogen Verticillium dahliae was recently demonstrated to exploit the VdAve1 effector to manipulate the host microbiota to promote vascular wilt disease in the absence of the corresponding immune receptor Ve1. We identify a multiallelic V. dahliae gene displaying c. 65% sequence similarity to VdAve1, named VdAve1-like (VdAve1L), which shows extreme sequence variation, including alleles that encode dysfunctional proteins, indicative of selection pressure to overcome host recognition. We show that the orphan cell surface receptor Ve2, encoded at the Ve locus, does not recognize VdAve1L. Additionally, we demonstrate that the full-length variant VdAve1L2 possesses antimicrobial activity, like VdAve1, yet with a divergent activity spectrum, that is exploited by V. dahliae to mediate tomato colonization through the direct suppression of antagonistic Actinobacteria in the host microbiota. Our findings open up strategies for more targeted biocontrol against microbial plant pathogens

Timing of extinction relative to acquisition: A parametric analysis of fear extinction in humans

Fear extinction is a reduction in conditioned fear following repeated exposure to the feared cue in the absence of any aversive event. Extinguished fear often reappears after extinction through spontaneous recovery. Animal studies suggest that spontaneous recovery can be abolished if extinction occurs within minutes of acquisition. However, a limited number of human extinction studies have shown that short interval extinction does not prevent the return of fear. For this reason, we performed an in-depth parametric analysis of human fear extinction using fear-potentiated startle. Using separate single-cue and differential conditioning paradigms, participants were fear conditioned and then underwent extinction either 10 min (Immediate) or 72 hr (Delayed) later. Testing for spontaneous recovery occurred 96 hr after acquisition. In the single cue paradigm, the Immediate and Delayed groups exhibited differences in context, but not fear, conditioning. With differential conditioning, there were no differences in context conditioning and the Immediate group displayed less spontaneous recovery. Thus, the results remain inconclusive regarding spontaneous recovery and the timing of extinction and are discussed in terms of performing translational studies of fear in humans

Late Cenozoic fluvial dynamics of the River Tana, Kenya, an uplift dominated record

The Late Cenozoic development of the River Tana in Kenya has been reconstructed for its central reach near its confluence with the River Mutonga, which drains the Mount Kenya region. Age control for this system has been provided by K-Ar and Ar-Ar dating. Between 3.21 and 2.65 Ma a major updoming occurred, in relation to the formation of the Kenyan rift valley. The tilting related to this doming has been reconstructed from lava flows that preserve former river gradients. Linear projection of these trends to the current rift valley rim suggests a net updoming of the eastern Gregory Rift valley by at least similar to 1 km during 3.21-2.65 Ma. In contrast, since 2.65 Ma the Tana system has been mainly subject to relatively minor epeirogenic uplift. Changing climatic conditions combined with continuing uplift yielded a typical staircase of strath terraces with at least 10 distinct levels. A more detailed reconstruction of the incision rates since 215 ka has been made, by correlating mineralogically fingerprinted volcaniclastic Tana deposits with dated tephras in a lake record. These volcaniclastic sediments were deposited during glacial periods, contemporaneous with lahars. The reconstructed incision rates for the three youngest terraces are similar to 0.1-0.2 mm a(-1), thus considerably faster than the overall average rate of valley incision since the Mid-Pliocene, of 0.06 mm a(-1). A plausible uplift history has been reconstructed using the estimated ages of the Tana terraces and marine terraces on the Indian Ocean coastline. The result suggests an increase in the rate of incision by the River Tana at similar to 0.9 Ma, an observation typical in most European river terrace staircases. The reconstructed Late Quaternary development of Tana valley indicates that a similar Quaternary uplift mechanism has operated in both Europe and East Kenya, suggesting a globally applicable process. (c) 2007 Elsevier Ltd. All rights reserved

An innovative Method to study Volatile Fission Products speciation in nuclear fuels under Severe Accident Conditions

International audienceThe development of realistic models for fission products behavior during a severe accident requires experimental data on fission products speciation into the fuel (as irradiated and during the transient) together with thermodynamic predictions. Due to the limitations in terms of experiments and characterization techniques available to study fission products speciation in nuclear irradiated fuels, simulant materials (SIMFUEL manufactured thanks to conventional process) are often used. However, these materials do not allow the study of volatile fission products because they are totally released during the sintering stage. In the case of SIMFUEL with implanted fission products surrogates, the chemical state and the corresponding evolution of the phases during the thermal sequence are not entirely representative of the real case. An innovative method to study volatile fission products speciation in SIMFUEL samples has thus been developed. Spark plasma sintering was used to synthesize dense SIMFUEL samples containing cesium in collaboration with the JRC-Karlsruhe within the frame of the GENTLE program. Thermodynamic calculations made using the TAF-ID v.6 associated with the FACTSage software enabled to determine the conditions in which to perform thermal treatments. In-temperature XAS experiments in conditions representative of a sevre accident are developed in collaboration with FAME-UHD beamline at the ESRF (Grenoble, France). Additional thermal treatments will also be performed to enable further characterizations (ceramography, SEM-EDX...)

Assessment of solid/liquid equilibria in the (U, Zr)O2+y system

Solid/liquid equilibria in the system UO2eZrO2 are revisited in this work by laser heating coupled with fast optical thermometry. Phase transition points newly measured under inert gas are in fair agreement with the early measurements performed by Wisnyi et al., in 1957, the only study available in the literature on the whole pseudo-binary system. In addition, a minimum melting point is identified here for compositions near (U0.6Zr0.4)O2þy, around 2800 K. The solidus line is rather flat on a broad range of compositions around the minimum. It increases for compositions closer to the pure end members, up to the melting point of pure UO2 (3130 K) on one side and pure ZrO2 (2970 K) on the other. Solid state phase transitions (cubic-tetragonal-monoclinic) have also been observed in the ZrO2-rich compositions X-ray diffraction. Investigations under 0.3 MPa air (0.063 MPa O2) revealed a significant decrease in the melting points down to 2500 Ke2600 K for increasing uranium content (x(UO2)> 0.2). This was found to be related to further oxidation of uranium dioxide, confirmed by X-ray absorption spectroscopy. For example, a typical oxidised corium composition U0.6Zr0.4O2.13 was observed to solidify at a temperature as low as 2493 K. The current results are important for assessing the thermal stability of the system fuel e cladding in an oxide based nuclear reactor, and for simulating the system behaviour during a hypothetical severe accident

A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell

Plasmodium falciparum exports ~10% of its proteome into its host erythrocyte to modify the host cell&rsquo;s physiology. The Plasmodium export element (PEXEL) motif contained within the N-terminus of most exported proteins directs the trafficking of those proteins into the erythrocyte. To reach the host cell, the PEXEL motif of exported proteins is processed by the endoplasmic reticulum (ER) resident aspartyl protease plasmepsin V. Then, following secretion into the parasite-encasing parasitophorous vacuole, the mature exported protein must be unfolded and translocated across the parasitophorous vacuole membrane by the Plasmodium translocon of exported proteins (PTEX). PTEX is a protein-conducting channel consisting of the pore-forming protein EXP2, the protein unfoldase HSP101, and structural component PTEX150. The mechanism of how exported proteins are specifically trafficked from the parasite&rsquo;s ER following PEXEL cleavage to PTEX complexes on the parasitophorous vacuole membrane is currently not understood. Here, we present evidence that EXP2 and PTEX150 form a stable subcomplex that facilitates HSP101 docking. We also demonstrate that HSP101 localises both within the parasitophorous vacuole and within the parasite&rsquo;s ER throughout the ring and trophozoite stage of the parasite, coinciding with the timeframe of protein export. Interestingly, we found that HSP101 can form specific interactions with model PEXEL proteins in the parasite&rsquo;s ER, irrespective of their PEXEL processing status. Collectively, our data suggest that HSP101 recognises and chaperones PEXEL proteins from the ER to the parasitophorous vacuole and given HSP101&rsquo;s specificity for the EXP2-PTEX150 subcomplex, this provides a mechanism for how exported proteins are specifically targeted to PTEX for translocation into the erythrocyte.</jats:p