Componentes lipofílicos y evaluación de las actividades citotóxicas y antioxidantes de Impatiens glandulifera Royle e Impantiet noli-tangere L. (Balsaminaceae)

The chemical composition of the lipophilic fractions of Impatiens glandulifera Royle and I. noli-tangere L. were analyzed by gas chromatography-mass spectrometry (GC-MS)., The study focused on the fatty acids, triterpenoids and sterols in the leaves, roots and seeds. Most of the identified compounds are new for these species. a-linolenic, oleic and palmitic acids were the most abundant in the fatty acid fractions, β-amyrin and 5a-lup-20(29)-en-3β-ol in the triterpenoid fractions, and β-sitosterol, spinasterol and chondrillasterol in the sterol fractions. The fatty acid and triterpenoid fractions showed strong antioxidant activity, similar to positive controls. Moreover, the triterpenoid fraction from I. noli-tangere seeds significantly inhibited HL-60 human leukemia cells. Other fractions showed moderate cytotoxicity. The present study suggests that I. glandulifera and I. noli-tangere are good source of omega-3 fatty acids, and they might be considered as antioxidant and chemopreventive agents.La composición química de las fracciones lipofílicas, centrada en los ácidos grasos, triterpenoides y esteroles de las partes aéreas, raíces y semillas de Impatiens glandulifera Royle e Impatient. noli-tangere L. se analizaron por cromatografía de gases-espectrometría de masas (GC-MS). La mayoría de los compuestos identificados son nuevos para estas especies. Los ácidos a-linolénico, oleico y palmítico fueron los más abundantes en las fracciones de ácidos grasos, β-amirina y 5a-lup-20 (29)-en-3β-ol en las fracciones triterpenoides, y β-sitosterol, espinasterol y condriplasterol en las fracciones de esteroles. Las fracciones de ácidos grasos y triterpenos mostraron una fuerte actividad antioxidante, similar a los controles positivos. Además, la fracción triterpenoidea de las semillas de I. noli-tangere inhibió significativamente las células de leucemia humana HL-60. Otras fracciones mostraron citotoxicidad moderada. El presente estudio sugiere que I. glandulifera e I. noli-tangere son la buena fuente de ácidos grasos omega-3, y podrían considerarse antioxidantes y agentes quimiopreventivos

The correlation of mutations and expressions of genes within the PI3K/Akt/mTOR pathway in breast cancer : a preliminary study

There is an urgent need to seek new molecular biomarkers helpful in diagnosing and treating breast cancer. In this elaboration, we performed a molecular analysis of mutations and expression of genes within the PI3K/Akt/mTOR pathway in patients with ductal breast cancer of various malignancy levels. We recognized significant correlations between the expression levels of the studied genes. We also performed a bioinformatics analysis of the data available on the international database TCGA and compared them with our own research. Studies on mutations and expression of genes were conducted using High-Resolution Melt PCR (HRM-PCR), Allele-Specific-quantitative PCR (ASP-qPCR), Real-Time PCR molecular methods in a group of women with ductal breast cancer. Bioinformatics analysis was carried out using web source Ualcan and bc-GenExMiner. In the studied group of women, it was observed that the prevalence of mutations in the studied PIK3CA and AKT1 genes was 29.63%. It was stated that the average expression level of the PIK3CA, PIK3R1, PTEN genes in the group of breast cancer patients is lower in comparison to the control group, while the average expression level of the AKT1 and mTOR genes in the studied group was higher in comparison to the control group. It was also indicated that in the group of patients with mutations in the area of the PIK3CA and AKT1 genes, the PIK3CA gene expression level is statistically significantly lower than in the group without mutations. According to our knowledge, we demonstrate, for the first time, that there is a very strong positive correlation between the levels of AKT1 and mTOR gene expression in the case of patients with mutations and without mutations

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show respectively positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA. Supplementary material 3 shows log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. RQ values were used in statistical analysis. Supplementary material 4 shows a profile of gene expression changes for CCRF/CEM cell line after 24-hour exposure to IC50

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show respectively positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA. Supplementary material 3 shows log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. RQ values were used in statistical analysis. Supplementary material 4 shows a profile of gene expression changes for CCRF/CEM cell line after 24-hour exposure to IC50

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show respectively positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA.Supplementary material 3 shows log RQ values for all studied gens. Presented logRQ values were used in statistical analysis. Supplementary material 4 shows a profile of gene expression changes for CCRF/CEM cell line after 24-hour exposure to IC50

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show respectively positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA. Supplementary material 3 shows log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. RQ values were used in statistical analysis. Supplementary material 4 shows a profile of gene expression changes for CCRF/CEM cell line after 24-hour exposure to IC50

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary materials: 1 and 2 show all positive correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA. Supplementary material 3 shows Log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. That values were used to conduct all statistical analysis of gene expression changes. Supplementary material 4 shows an example profile of gene expression changes in CCRF/CEM cell line

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show all positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA.Supplementary material 3 show Log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. RQ values were used to conduct statistical analysis. Supplementary material 4 shows an example of gene expression changes profile for CCRF/CEM cell line

Data for: Effect of sodium dichloroacetate on apoptotic gene expression in human leukemia cell lines

Supplementary material 1 and 2 show respectively positive and negative correlations between changes of gene expression generated using Spearman’s analysis for all 4 cell lines after 24, 48 and 72 hours of exposure of cells to DCA. Supplementary material 3 shows log RQ values for all examined genes in four cell lines after 24, 48 and 72 hours. RQ values were used in statistical analysis. Supplementary material 4 shows a profile of gene expression changes for CCRF/CEM cell line after 24-hour exposure to IC50.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV