Natural ligands of hamster aphrodisin

Abstract 75National audienc

Natural ligands of hamster aphrodisin

Abstract 75National audienc

Study of the flavour and of flavour precursors in cured cooked ham. Relation between flavour and composition

International audienc

Study of the flavour and of flavour precursors in cured cooked ham. Relation between flavour and composition

International audienc

Bacterial protein signals are associated with Crohn's disease

Objective No Crohn’s disease (CD) molecular maker has advanced to clinical use, and independent lines of evidence support a central role of the gut microbial community in CD. Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. Design We first developed and validated a workflow—including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS—to discover protein signals from CD-associated gut microbial communities. Then we used selected reaction monitoring (SRM) to confirm a set of candidates. In parallel, we used 16S rRNA gene sequencing for an integrated analysis of gut ecosystem structure and functions. Results Our 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. Most overabundant proteins could be confirmed using SRM. They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis

Identification of human olfactory cleft mucus proteins using proteomic analysis

International audienc

Evidence of an odorant-binding protein in the human olfactory mucus : location, structural characterization , and odorant-binding properties

International audienc

Evidence of a human odorant-binding protein in the olfactory mucus :location, characterization and potential role as an odorant carrier

International audienc

Specific expression of olfactory binding protein in the aerial olfactory cavity of adult and developing Xenopus

International audienc

Structural recognition between olfactory receptors, olfactory-binding proteins and odorants

National audienc