101 research outputs found
Study of Iron in Magnetotactic Bacteria
Bacteria have recently been discovered directing their movements by sensing the earth\u27s magnetic field. They contain arrays of crystals of lodestone or magnetite which they synthesize from iron in the ocean. The discovery of the same type of crystals in the abdomens of honey bees and the brains of homing pigeons suggests that nature is not oblivious to the earth\u27s magnetic field. The biological mechanism for sensing magnetic fields has never been determined. These bacteria are capable of biologically synthesizing highly purified single domain magnets that would be very expensive to produce chemically.
The tiny perfect magnetite crystals made by the bacteria possibly could be utilized in microcircuitry. In addition, because these bacteria are attracted to metal surfaces surrounded by distortions in the earth\u27s magnetic field, they may play a role in ship fouling. The unique physical properties of the single domain ferromagnetic crystals of magnetite produced by these bacteria are being analyzed of heavy metal storage could be important in recovering metals from ore and nuclear wastes; this is a continuing research project supported by the Office of Naval Research
A Birefringence Relaxation Determination of Rotational Diffusion of Magnetotactic Bacteria
The orientational relaxation of the magnetotactic bacterium Aquaspirillum magnetotacticum is observed by the decay of the optical birefringence upon switching off an aligning magnetic field. The data yield a rotational diffusion constant Dr ≅ 0.13 s1 and information about cell sizes that is consistent with optical microscopy data
Fe\u3csub\u3e3\u3c/sub\u3eO\u3csub\u3e4\u3c/sub\u3e Precipitation in Magnetotactic Bacteria
Using Mössbauer resonance spectroscopy of 57Fe, we have determined the nature and distribution of major iron compounds in the magnetotactic bacterium Aquaspirillum magnetotacticum. In addition to magnetite (Fe3O4), cells contained a low-density hydrous ferric oxide, a high-density hydrous ferric oxide (ferrihydrite), and ferrous iron. Analysis at different temperatures of whole cells harvested early and late in growth, of mutant cells unable to synthesize magnetite, and of cell fractions enriched in 57Fe indicated that Fe3O4 precipitation resulted from partial reduction of the high-density hydrous ferric oxide precursor
Magnetic Properties of Magnetotactic Bacteria
This paper reports on the magnetic properties of magnetosomes in the freshwater magnetotactic bacterium Aquaspirillum magnetotacticum. The magnetosomes are well crystallized particles of magnetite with dimensions of 40 to 50 nm, which are arranged within the cells in a single linear chain and are within the single-magnetic-domain (SD) size range for magnetite. A variety of magnetic properties have been measured for two samples of dispersions of freeze-dried cells consisting of (1) whole cells (M-1) and (2) magnetosomes chains separated from cells (M-2). An important result is that the acquisition and demagnetization of various type of remanent magnetizations are markedly different for the two samples and suggest that remanence is substantially affected by magnetostatic interactions. Interactions are likely to be much more important in M-2 because the extracted magnetosome chains are no longer separated from one another by the cell membrane and cytoplasm. Other experimental data for whole cells agree with predictions based on the chain of spheres model for magnetization reversal. This model is consistent with the unique linear arrangement of equidimensional particles in A. magnetotacticum. The magnetic properties of bacterial and synthetic magnetites are compared and the paleomagnetic implications are discussed
Microaerobic Conditions Are Required for Magnetite Formation Within \u3ci\u3eAquaspirillum magnetotacticum\u3c/i\u3e
The amount of magnetite (Fe3O4) within magnetosomes of the microaerophilic bacterium Aquaspirillum magnetotacticum varies with oxygen and nitrogen supply. The development of optical methods for directly measuring cell magnetism in culture samples has enabled us to quantitate bacterial Fe3O4 yields. We measured final cell yields, average cell magnetic moments, and magnetosome yields of growing cells. Cultures were grown with NO3-, NH4+, or both, in sealed, unshaken vials with initial headspace Po2 values ranging from 0 (trace) to 21 kPa. More than 50% of cells had detectable magnetosomes only when grown in the range of 0.5-5.0 kPa O2. Optimum cell magnetism (and Fe3O4 formation) occurred under microaerobic conditions (initial headspace Po2 of 0.5-1 kPa) regardless of the N source. At optimal conditions for Fe3O4 formation, denitrifying cultures produced more of this mineral than those growing with O2 as the sole terminal electron acceptor. This suggests that competition for O2 exists between processes involving respiratory electron disposal and Fe3O4 formation. Oxygen may also be required for Fe3O4 formation by other species of magnetotactic bacteria. Bacterial Fe3O4 appears to persist in sediments after death and lysis of cells. The presence of bacterial Fe3O4 in the fossil and paleomagnetic records may be of use as a retrospective indicator of sedimentation that has occurred in microaerobic waters
Bats Use Magnetite to Detect the Earth's Magnetic Field
While the role of magnetic cues for compass orientation has been confirmed in numerous animals, the mechanism of detection is still debated. Two hypotheses have been proposed, one based on a light dependent mechanism, apparently used by birds and another based on a “compass organelle” containing the iron oxide particles magnetite (Fe3O4). Bats have recently been shown to use magnetic cues for compass orientation but the method by which they detect the Earth's magnetic field remains unknown. Here we use the classic “Kalmijn-Blakemore” pulse re-magnetization experiment, whereby the polarity of cellular magnetite is reversed. The results demonstrate that the big brown bat Eptesicus fuscus uses single domain magnetite to detect the Earths magnetic field and the response indicates a polarity based receptor. Polarity detection is a prerequisite for the use of magnetite as a compass and suggests that big brown bats use magnetite to detect the magnetic field as a compass. Our results indicate the possibility that sensory cells in bats contain freely rotating magnetite particles, which appears not to be the case in birds. It is crucial that the ultrastructure of the magnetite containing magnetoreceptors is described for our understanding of magnetoreception in animals
Dose study of the multikinase inhibitor, LY2457546, in patients with relapsed acute myeloid leukemia to assess safety, pharmacokinetics, and pharmacodynamics
Relevance theory, pragmatic inference and cognitive architecture
Relevance Theory (RT: Sperber & Wilson, 1986) argues that human language comprehension processes tend to maximize “relevance”, and postulates that there is a relevance-based procedure that a hearer follows when trying to understand an utterance. Despite being highly influential, RT has been criticized for its failure to explain how speaker-related information, either the speaker’s abilities or her/his preferences, is incorporated into the hearer’s inferential, pragmatic process. An alternative proposal is that speaker-related information gains prominence due to representation of the speaker within higher-level goal-directed schemata. Yet the goal-based account is still unable to explain clearly how cross-domain information, for example linguistic meaning and speaker-related knowledge, is integrated within a modular system. On the basis of RT’s cognitive requirements, together with contemporary cognitive theory, we argue that this integration is realized by utilizing working memory and that there exist conversational constraints with which the constructed utterance interpretation should be consistent. We illustrate our arguments with a computational implementation of the proposed processes within a general cognitive architecture
Are C-Reactive Protein Associated Genetic Variants Associated with Serum Levels and Retinal Markers of Microvascular Pathology in Asian Populations from Singapore?
Introduction:C-reactive protein (CRP) levels are associated with cardiovascular disease and systemic inflammation. We assessed whether CRP-associated loci were associated with serum CRP and retinal markers of microvascular disease, in Asian populations.Methods:Genome-wide association analysis (GWAS) for serum CRP was performed in East-Asian Chinese (N = 2,434) and Malays (N = 2,542) and South-Asian Indians (N = 2,538) from Singapore. Leveraging on GWAS data, we assessed, in silico, association levels among the Singaporean datasets for 22 recently identified CRP-associated loci. At loci where directional inconsistencies were observed, quantification of inter-ethnic linkage disequilibrium (LD) difference was determined. Next, we assessed association for a variant at CRP and retinal vessel traits [central retinal artery equivalent (CRAE) and central retinal vein equivalent (CRVE)] in a total of 24,132 subjects of East-Asian, South-Asian and European ancestry.Results:Serum CRP was associated with SNPs in/near APOE, CRP, HNF1A and LEPR (p-values ≤4.7×10-8) after meta-analysis of Singaporean populations. Using a candidate-SNP approach, we further replicated SNPs at 4 additional loci that had been recently identified to be associated with serum CRP (IL6R, GCKR, IL6 and IL1F10) (p-values ≤0.009), in the Singaporean datasets. SNPs from these 8 loci explained 4.05% of variance in serum CRP. Two SNPs (rs2847281 and rs6901250) were detected to be significant (p-value ≤0.036) but with opposite effect directions in the Singaporean populations as compared to original European studies. At these loci we did not detect significant inter-population LD differences. We further did not observe a significant association between CRP variant and CRVE or CRAE levels after meta-analysis of all Singaporean and European datasets (p-value >0.058).Conclusions:Common variants associated with serum CRP, first detected in primarily European studies, are also associated with CRP levels in East-Asian and South-Asian populations. We did not find a causal link between CRP and retinal measures of microvascular disease
A Structured Model of Video Reproduces Primary Visual Cortical Organisation
The visual system must learn to infer the presence of objects and features in the world from the images it encounters, and as such it must, either implicitly or explicitly, model the way these elements interact to create the image. Do the response properties of cells in the mammalian visual system reflect this constraint? To address this question, we constructed a probabilistic model in which the identity and attributes of simple visual elements were represented explicitly and learnt the parameters of this model from unparsed, natural video sequences. After learning, the behaviour and grouping of variables in the probabilistic model corresponded closely to functional and anatomical properties of simple and complex cells in the primary visual cortex (V1). In particular, feature identity variables were activated in a way that resembled the activity of complex cells, while feature attribute variables responded much like simple cells. Furthermore, the grouping of the attributes within the model closely parallelled the reported anatomical grouping of simple cells in cat V1. Thus, this generative model makes explicit an interpretation of complex and simple cells as elements in the segmentation of a visual scene into basic independent features, along with a parametrisation of their moment-by-moment appearances. We speculate that such a segmentation may form the initial stage of a hierarchical system that progressively separates the identity and appearance of more articulated visual elements, culminating in view-invariant object recognition
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