Additional file 4 of Vascular adhesion protein-1 expression is reduced in the intestines of infants with necrotizing enterocolitis: an observational research study

Additional file 4: Supplementary Fig. 4. Difference in VAP-1 expression between alive and deceased NEC infants. No significant difference in VAP-1 Area % was found (Alive = 0.065 ± 0.034; Deceased = 0.065 ± 0.038, t(1,40) = − 0.0061, p = 0.99). NS signifies p > 0.05

Additional file 3 of Vascular adhesion protein-1 expression is reduced in the intestines of infants with necrotizing enterocolitis: an observational research study

Additional file 3: Supplementary Fig. 3. Linear regression indicated a strong correlation between mean VAP-1 expression and VAP-1 area % for a) all included infants (R2 = 0.61, F(1, 66) = 101.4, p < 0.001, n = 68), b) NEC infants (R2 = 0.52, F(1, 40) = 43.69, p < 0.001, n = 42) and for c) control infants (R2 = 0.49, F(1, 24) = 22.88, p < 0.001, n = 26)

Additional file 1 of Vascular adhesion protein-1 expression is reduced in the intestines of infants with necrotizing enterocolitis: an observational research study

Additional file 1: Supplementary Fig. 1. Microscopic image of a representative tissue sample with original magnification

Additional file 2 of Vascular adhesion protein-1 expression is reduced in the intestines of infants with necrotizing enterocolitis: an observational research study

Additional file 2: Supplementary Fig. 2. Significant difference in VAP-1 expression. There were significant differences in a) VAP-1 area % (NEC = 0,065 ± 0,035; controls = 0,11 ± 0,042, t(1,66) = 4.89, p < 0.001), b) VAP-1 mean (NEC = 80,74 ± 3,81; controls = 86,29 ± 6,06, t(1,66) = 4.65, p < 0.001), c) VAP-1 median (NEC = 76,44 ± 3,39; controls = 81,92 ± 6,09, t(1,66) = 4.77, p < 0.001), d) VAP-1 mode (NEC = 64,79 ± 1,07; controls = 62,33 ± 4,09, t(1,66) = 3.72, p < 0.001) and e) VAP-1 max (NEC = 150,99 ± 15,3; controls = 162,45 ± 12,3, t(1,66) = 3.23, p < 0.01). * signifies p < 0.05, ** signifies p < 0.01 and *** signifies p < 0.001

Gene cloud displaying the 500 most significantly differentially expressed transcripts.

<p>Font size is proportional to the highest change in expression and inversely proportional to the likelihood of the result.</p

Comparison of differentially expressed transcribed sequences.

<p>Comparison of the differences in expression between vVP1Q164K, CVB2Owt and mock, at 48 hours post infection. Difference in expression that (only) occurs in: (pink) vVP1Q164K compared to CVB2Owt; (orange) vVP1Q164K compared to both CVB2Owt and mock; (yellow) vVP1Q164K compared to mock; (purple) CVB2Owt compared to both vVP1Q164K and mock; (white) between all conditions; (green) mock compared to both vVP1Q164K and CVB2Owt; (blue) CVB2Owt compared to mock.</p

Relative amounts of intracellular RNA.

<p>(A) All sequences were mapped against the CVB2O and human genome to obtain the proportions of viral to human RNA in each sample. Sequences that did not match either genome are denoted <i>other</i>. (B) The intracellular amounts of viral RNA have been normalized against the amount in the first sample of the triplicate of the cells infected with vVP1Q164K and incubated for 24 hours. Error bars indicate 95% confidence interval.</p

The 20 transcripts most differentially expressed between vVP1Q164K (48 h) and CVB2Owt.

<p>The conditions are normalized to mock-cell expression levels.</p

Differentially expressed transcripts at alpha 0.01.

<p>The number of transcripts that are differentially expressed compared to the other conditions.</p

Additional file 7: of Comparative genomic analyses of freshly isolated Giardia intestinalis assemblage A isolates

Strcutural modellling of two Giardia BPI-like proteins. (DOCX 486 kb